A Simple and Efficient Procedure for Generating Random Point Mutations and for Codon Replacements Using Mixed Oligodeoxynucleotides

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA

1985 • 849 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

DNA MISMATCH CORRECTION

1987 • 427 citations

Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.

1980 • 391 citations

An improved filamentous helper phage for generating single-stranded plasmid DNA

1986 • 348 citations

IN VITRO MUTAGENESIS

1985 • 338 citations

Repair tracts in mismatched DNA heteroduplexes.

1976 • 300 citations

The sequence of the DNAs coding for the mating-type loci of saccharomyces cerevisiae

1981 • 286 citations

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1985 • 270 citations

MISMATCH REPAIR IN ESCHERICHIA COLI

1986 • 253 citations

Conversion of the M13 Viral Single Strand to the Double-stranded Replicative Forms by Purified Proteins

1974 • 202 citations

Strategies and Applications of in Vitro Mutagenesis

1985 • 192 citations

Uracil incorporation: A source of pulse-labeled DNA fragments in the replication of the Escherichia coli chromosome

1978 • 151 citations

DIRECTED MUTAGENESIS

1981 • 142 citations

Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.

1984 • 128 citations

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length

1979 • 117 citations

Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities

1984 • 115 citations

A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.

1986 • 114 citations

Efficient correction of a mutation by use of chemically synthesized DNA.

1978 • 112 citations

Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5‘ of an ATG initiation codon

1983 • 112 citations

A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides

1986 • 112 citations

Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria.

1986 • 112 citations

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71.

1986 • 73 citations

Ff coliphages: structural and functional relationships.

1986 • 61 citations

Studies of the radical species in compound ES of cytochrome c peroxidase altered by site-directed mutagenesis.

1986 • 60 citations

Homoeo-domain homology in yeast MATα2 is essential for repressor activity

1986 • 57 citations

Simplified DNA manupulations based on in vitro mutagenesis

1985 • 53 citations

Analysis of the γδ res site

1984 • 48 citations

Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs.

1985 • 37 citations

Site-directed mutagenesis

1986 • 21 citations

Fusion of restriction termini using synthetic adaptor oligonucleotides

1982 • 11 citations