Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Experiments in molecular genetics

1972 • 28,260 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

A new method for sequencing DNA.

1977 • 7,941 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

A membrane-filter technique for the detection of complementary DNA

1966 • 3,996 citations

Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose

1984 • 1,697 citations

Polyacrylamide Gel Electrophoresis of Viral Proteins

1971 • 1,477 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron.

1985 • 268 citations

Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium

1985 • 225 citations

Identification of Tissue-Specific Nuclear Antigens Transferred to Nitrocellulose from Polyacrylamide Gels

1981 • 178 citations

Isopenicillin N synthetase of Penicillium chrysogenum, an enzyme that converts delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to isopenicillin N

1985 • 134 citations

Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

1984 • 131 citations

Extrachromosomal genetics of Cephalosporium acremonium

1982 • 66 citations

Secondary products of metabolism

1979 • 50 citations

Extrachromosomal genetics of Cephalosporium acremonium

1982 • 40 citations

Cell-free biosynthesis of penicillins. Conversion of peptides into new .beta.-lactam antibiotics

1981 • 38 citations

N‐terminal amino acid sequence and some properties of isopenicillin‐N synthetase from Cephalosporium acremonium

1985 • 31 citations

Penicillin biosynthesis. Dual pathways from a modified substrate

1983 • 25 citations

Penicillin biosynthesis: active site mapping with aminoadipoylcysteinylvaline variants

1984 • 22 citations

Enzymatic Synthesis of a Sulfur-Analog of Penicillin Using the “cyclase” of Cephalosporium Acremonium

1984 • 20 citations

Enzymatic conversion of the unnatural tripeptide .DELTA.-(D-.ALPHA.-aminoadipyl)-L-cysteinyl-D-valine to .BETA.-lactam antibiotics.

1984 • 12 citations