A Mutation in an ATP-binding Loop of Saccharomyces cerevisiae Actin (S14A) Causes a Temperature-sensitive Phenotype in Vivo and in Vitro

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[1] Getting started with yeast

1991 • 2,456 citations

Effects of cytochalasin and phalloidin on actin.

1987 • 1,989 citations

Atomic structure of the actin: DNase I complex

1990 • 1,897 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin

1975 • 1,047 citations

Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.

1984 • 838 citations

Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments.

1986 • 828 citations

Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.

1984 • 827 citations

An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins.

1992 • 805 citations

Head to tail polymerization of actin

1976 • 692 citations

Phenotypic analysis of temperature-sensitive yeast actin mutants

1985 • 512 citations

Actin Polymerization and ATP Hydrolysis

1987 • 439 citations

Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway

1991 • 411 citations

Actin: protein structure and filament dynamics.

1991 • 309 citations

[52] Staining of bud scars and other cell wall chitin with Calcofluor

1991 • 272 citations

Interaction of actin with phalloidin:

1975 • 272 citations

Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.

1993 • 252 citations

Development of cell polarity in budding yeast

1991 • 252 citations

The kinetics of actin nucleation and polymerization.

1983 • 245 citations

Osmotic stress and the yeast cytoskeleton: phenotype-specific suppression of an actin mutation.

1992 • 245 citations

Kinetic evidence for a monomer activation step in actin polymerization

1983 • 206 citations

A Conformational Change in the Actin Subunit Can Change the Flexibility of the Actin Filament

1993 • 203 citations

THE MOLECULAR EVOLUTION OF ACTIN

1986 • 172 citations

A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility

1992 • 168 citations

Biochemical and Structural Studies of Actomyosin-like Proteins from Non-Muscle Cells

1974 • 168 citations

Centractin is an actin homologue associated with the centrosome

1992 • 164 citations

Structural basis for the destabilization of F-actin by phosphate release following ATP hydrolysis

1992 • 142 citations

Tightly-bound divalent cation of actin

1992 • 140 citations

Localization of the tightly bound divalent‐cation‐dependent and nucleotide‐dependent conformation changes in G‐actin using limited proteolytic digestion

1993 • 139 citations

Phalloidin enhances actin assembly by preventing monomer dissociation.

1984 • 130 citations

[51] Staining of actin with fluorochrome-conjugated phalloidin

1991 • 129 citations

New yeast actin-like gene required late in the cell cycle

1992 • 124 citations

A fluorescent probe for conformational changes in skeletal muscle G-actin.

1980 • 120 citations

Removal of the amino-terminal acidic residues of yeast actin. Studies in vitro and in vivo.

1992 • 112 citations

ATP binding to a protease-resistant core of actin.

1976 • 110 citations

Yeast actin with a mutation in the "hydrophobic plug" between subdomains 3 and 4 (L266D) displays a cold-sensitive polymerization defect.

1993 • 107 citations

The Mg2+-induced conformational change in rabbit skeletal muscle G-actin.

1982 • 103 citations

The effects of Mg2+ at the high-affinity and low-affinity sites on the polymerization of actin and associated ATP hydrolysis.

1986 • 101 citations

Fluorescence measurements of the binding of cations to high-affinity and low-affinity sites on ATP-G-actin.

1986 • 97 citations

Enhanced stimulation of myosin subfragment 1 ATPase activity by addition of negatively charged residues to the yeast actin NH2 terminus.

1993 • 97 citations

Subtilisin-cleaved actin: polymerization and interaction with myosin subfragment 1

1989 • 90 citations

Proteolysis and structure of skeletal muscle actin.

1984 • 88 citations

Role of magnesium in the relaxation of myofibrils

1969 • 86 citations

Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe that encodes a protein related to actin.

1992 • 85 citations

The cytoskeleton: A practical approach

1993 • 82 citations

The mechanisms of ATP hydrolysis accompanying the polymerization of Mg-actin and Ca-actin.

1987 • 81 citations

Localization of the phalloidin and nucleotide‐binding sites on actin

1987 • 72 citations

Effect of Acanthamoeba profilin on the pre-steady state kinetics of actin polymerization and on the concentration of F-actin at steady state.

1983 • 66 citations

Bound-cation exchange affects the lag phase in actin polymerization

1984 • 61 citations

Cooperative effects on filament stability in actin modified at the C‐terminus by substitution or truncation

1993 • 57 citations

Calcium and magnesium binding to thin and thick filaments in skinned muscle fibres: electron probe analysis

1982 • 55 citations

Behavior of divalent cations and nucleotides bound to F-actin

1969 • 55 citations

The tightly bound divalent cation regulates actin polymerization

1983 • 53 citations

Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo.

1991 • 49 citations

The cytoskeleton: A practical approach

1992 • 45 citations

Photoaffinity labeling of the nucleotide binding site of actin

1986 • 35 citations

The enhanced ATPase activity of glutathione-substituted actin provides a quantitative approach to filament stabilization.

1990 • 35 citations

Role of ATP-bound divalent metal ion in the conformation and function of actin. Comparison of Mg-ATP, Ca-ATP, and metal ion-free ATP-actin.

1991 • 32 citations

Functional, chymotryptically split actin and its interaction with myosin subfragment 1

1987 • 32 citations

Nucleotide exchange and rheometric studies with F-actin prepared from ATP- or ADP-monomeric actin

1993 • 29 citations

Conformational changes in subdomain‐2 of G‐actin upon polymerization into F‐actin and upon binding myosin subfragment‐1

1993 • 25 citations

Effect of bound-nucleotide substitution on the properties of F-actin

1969 • 20 citations