Actin Polymerization and ATP Hydrolysis

Dynamic instability of microtubule growth

1984 • 3,187 citations

ACTIN AND ACTIN-BINDING PROTEINS. A CRITICAL EVALUATION OF MECHANISMS AND FUNCTIONS

1986 • 1,493 citations

Disruption of the Dictyostelium Myosin Heavy Chain Gene by Homologous Recombination

1987 • 984 citations

Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments.

1986 • 828 citations

Actin polymerization and its regulation by proteins from nonmuscle cells.

1982 • 739 citations

At least six different actins are expressed in a higher mammal: An analysis based on the amino acid sequence of the amino-terminal tryptic peptide

1978 • 738 citations

Head to tail polymerization of actin

1976 • 692 citations

Antisense RNA Inactivation of Myosin Heavy Chain Gene Expression in Dictyostelium discoideum

1987 • 677 citations

Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling.

1985 • 637 citations

Nonmuscle Actin-Binding Proteins

1985 • 589 citations

Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

1980 • 567 citations

Three-dimensional reconstruction of F-actin, thin filaments and decorated thin filaments

1970 • 552 citations

Visualization of the dynamic instability of individual microtubules by dark-field microscopy

1986 • 547 citations

Organization, Chemistry, and Assembly of the Cytoskeletal Apparatus of the Intestinal Brush Border

1985 • 525 citations

Biochemistry of actomyosin-dependent cell motility (a review).

1978 • 489 citations

Muscle Contraction and Free Energy Transduction in Biological Systems

1985 • 446 citations

Direct measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores.

1981 • 298 citations

Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules

1976 • 281 citations

Kinetic analysis of guanosine 5'-triphosphate hydrolysis associated with tubulin polymerization

1981 • 270 citations

Interconvertible Enzyme Cascades in Cellular Regulation

1980 • 257 citations

The kinetics of actin nucleation and polymerization.

1983 • 245 citations

Kinetics of the cooperative association of actin to actin filament

1975 • 244 citations

RELATIONSHIPS BETWEEN CHEMICAL AND MECHANICAL EVENTS DURING MUSCULAR CONTRACTION

1986 • 241 citations

Kinetic evidence for a monomer activation step in actin polymerization

1983 • 206 citations

Complete nucleotide sequence of a soybean actin gene

1982 • 197 citations

Guanosinetriphosphatase activity of tubulin associated with microtubule assembly.

1977 • 174 citations

Contribution of actin to the structure of the cytoplasmic matrix.

1984 • 172 citations

Elongation of actin filaments is a diffusion-limited reaction at the barbed end and is accelerated by inert macromolecules.

1986 • 167 citations

Direct evidence for ADP-inorganic phosphate-F-actin as the major intermediate in ATP-actin polymerization. Rate of dissociation of inorganic phosphate from actin filaments

1986 • 160 citations

Fragmentation of actin filaments

1982 • 150 citations

Evidence for an ATP cap at the ends of actin filaments and its regulation of the F-actin steady state.

1984 • 149 citations

Binding of phosphate to F-ADP-actin and role of F-ADP-Pi-actin in ATP-actin polymerization.

1988 • 142 citations

Direct measurement of critical concentrations and assembly rate constants at the two ends of an actin filament

1983 • 139 citations

The amino acid sequence of Physarum actin

1978 • 137 citations

Mechanism of K+-induced actin assembly.

1982 • 132 citations

Isolation and characterization of the actin gene from Tetrahymena thermophila

1986 • 128 citations

The rate constant for ATP hydrolysis by polymerized actin

1984 • 127 citations

Evidence that myosin does not contribute to force production in chromosome movement.

1982 • 125 citations

Polymerization of actin: mechanism of the Mg2+-induced process at pH 8 and 20 degrees C.

1983 • 123 citations

A fluorescent probe for conformational changes in skeletal muscle G-actin.

1980 • 120 citations

Actin and Tubulin Polymerization: The Use of Kinetic Methods to Determine Mechanism

1985 • 119 citations

The structure of F-actin

1985 • 118 citations

Tetrahymena actin

1987 • 109 citations

The Mg2+-induced conformational change in rabbit skeletal muscle G-actin.

1982 • 103 citations

Three-dimensional structure of the complex of skeletal muscle actin and bovine pancreatic DNAse I at 6-A resolution.

1981 • 100 citations

Fluorescence measurements of the binding of cations to high-affinity and low-affinity sites on ATP-G-actin.

1986 • 97 citations

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence for Pi binding to a force-generating state.

1986 • 97 citations

Effect of villin on the kinetics of actin polymerization

1984 • 95 citations

F-actin is intermolecularly crosslinked by N,N'-p-phenylenedimaleimide through lysine-191 and cysteine-374.

1984 • 83 citations

The mechanisms of ATP hydrolysis accompanying the polymerization of Mg-actin and Ca-actin.

1987 • 81 citations

Interaction of cytochalasin D with actin filaments in the presence of ADP and ATP.

1986 • 80 citations

A model for actin polymerization and the kinetic effects of ATP hydrolysis.

1985 • 79 citations

Steady-state theory of the interference of GTP hydrolysis in the mechanism of microtubule assembly.

1983 • 78 citations

High affinity binding of divalent cation to actin monomer is much stronger than previously reported

1986 • 70 citations

The influence of poly(ethylene glycol) 6000 on the properties of skeletal-muscle actin

1983 • 70 citations

Rate of treadmilling of actin filaments in vitro

1986 • 68 citations

Assembly and dynamics of the actin filament system in nonmuscle cells

1986 • 68 citations

Polymerization of ADP-actin and ATP-actin under sonication and characteristics of the ATP-actin equilibrium polymer.

1985 • 65 citations

Bound-cation exchange affects the lag phase in actin polymerization

1984 • 61 citations

Cytoplasmic concentrations of inorganic phosphate affect the critical concentration for assembly of actin in the presence of cytochalasin D or ADP

1986 • 59 citations

Rate constants for actin polymerization in ATP determined using cross-linked actin trimers as nuclei.

1984 • 56 citations

X-ray diffraction evidence that actin is a 100 Å filament

1984 • 56 citations

Structure of the actin molecule determined from electron micrographs of crystalline actin sheets with a tentative alignment of the molecule in the actin filament

1983 • 54 citations

Measurement of Pi dissociation from actin filaments following ATP hydrolysis using a linked enzyme assay

1987 • 51 citations

Presence of oligomers at subcritical actin concentrations

1985 • 48 citations

A consistent picture of the actin filament related to the orientation of the actin molecule.

1983 • 45 citations

Inorganic phosphate promotes relaxation of chemically skinned smooth muscle of guinea-pigTaenia coli

1981 • 43 citations

Formation of actin dimers as studied by small angle neutron scattering.

1986 • 42 citations

Preparation and polymerization of skeletal muscle ADP-actin.

1984 • 42 citations

Synchrotron x-ray diffraction studies of actin structure during polymerization.

1987 • 39 citations

The interaction between ATP-actin and ADP-actin. A tentative model for actin polymerization.

1985 • 37 citations

Exposure of actin thiols by the removal of tightly held calcium ions.

1985 • 37 citations

Actin oligomers below the critical concentration detected by fluorescence photobleaching recovery

1985 • 32 citations

Evidence that F-actin can hydrolyze ATP independent of monomer-polymer end interactions.

1984 • 30 citations

ATP hydrolysis by the gelsolin-actin complex and at the pointed ends of gelsolin-capped filaments.

1986 • 30 citations

Nonlinear increase of elongation rate of actin filaments with actin monomer concentration

1986 • 29 citations

Under physiological conditions actin disassembles slowly from the nonpreferred end of an actin filament.

1983 • 27 citations

Theoretical study of a model for the ATP cap at the end of an actin filament

1986 • 26 citations

Interaction of plasma gelsolin with ADP-actin.

1986 • 21 citations

Kinetics of actin elongation and depolymerization at the pointed end

1987 • 21 citations

Altered secretion and accumulation of kidney glycosphingolipids by mouse pigmentation mutants with lysosomal dysfunctions.

1985 • 19 citations

Towards an alignment of the actin molecule within the actin filament

1984 • 17 citations

Concerning the anomalous kinetic behavior of microtubules.

1985 • 14 citations

Evaluation of the actin filament length from the time course of the depolymerization process

1986 • 8 citations

An Intermediate State of G-Actin between Native and Denatured: Polymerization Rate Decreases but Extent of Polymerization Remains Unchanged1

1986 • 7 citations

54Mn2+ as a tracer of the polymerization of actin. Intermediate oligomers condense to give F-actin

1984 • 6 citations

A mechanism for the selective preservation of homogeneous. F(ATP) actin.

1984 • 5 citations

The influence of adenosine triphosphate, adenosine diphosphate and cytochalasin B on nucleotide exchange of F-actin. Evidence that treadmilling is not involved

1985 • 4 citations