Comparison of pH Dependencies of the Tetrahymena Ribozyme Reactions with RNA 2'-Substituted and Phosphorothioate Substrates Reveals a Rate-Limiting Conformational Step

Catalysis in chemistry and enzymology

1969 • 3,236 citations

Hydrogen Ion Buffers for Biological Research^*

1966 • 2,690 citations

Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena

1982 • 2,241 citations

A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphatase

1960 • 1,407 citations

A general two-metal-ion mechanism for catalytic RNA.

1993 • 1,125 citations

SELF-SPLICING OF GROUP I INTRONS

1990 • 852 citations

The Intervening Sequence RNA of Tetrahymena Is an Enzyme

1986 • 498 citations

Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli

1987 • 490 citations

Metal ion catalysis in the Tetrahymena ribozyme reaction

1993 • 386 citations

An induced-fit kinetic mechanism for DNA replication fidelity: direct measurement by single-turnover kinetics

1991 • 364 citations

The guanosine binding site of the Tetrahymena ribozyme

1989 • 356 citations

Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. Kinetic description of the reaction of an RNA substrate complementary to the active site

1990 • 280 citations

A Study of the Substrate Specificity and Other Properties of the Alkaline Phosphatase of Escherichia coli

1962 • 272 citations

Sequence-specific endoribonuclease activity of the Tetrahymena ribozyme: enhanced cleavage of certain oligonucleotide substrates that form mismatched ribozyme-substrate complexes

1988 • 251 citations

Ribozyme-catalyzed and nonenzymic reactions of phosphate diesters: rate effects upon substitution of sulfur for a nonbridging phosphoryl oxygen atom

1991 • 230 citations

RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme

1992 • 182 citations

Kinetic Evidence for the Formation of Acyl-Enzyme Intermediates in the α-Chymotrypsin-Catalyzed Hydrolyses of Specific Substrates

1964 • 174 citations

Dynamics of Ribozyme Binding of Substrate Revealed by Fluorescence-Detected Stopped-Flow Methods

1992 • 170 citations

Limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta

1991 • 169 citations

The Intrinsic Pk_A-Values of Functional Groups in Enzymes: Improper Deductions from the Ph-Dependence of Steady-State Parameter

1976 • 168 citations

Ribozyme recognition of RNA by tertiary interactions with specific ribose 2′-OH groups

1991 • 165 citations

Stereochemistry of RNA Cleavage by the Tetrahymena Ribozyme and Evidence that the Chemical Step Is Not Rate-Limiting

1989 • 155 citations

Phosphorus-31 nuclear magnetic resonance study of alkaline phosphatase: the role of inorganic phosphate in limiting the enzyme turnover rate at alkaline pH

1976 • 137 citations

pH Variation of isotope effects in enzyme-catalyzed reactions. 1. Isotope- and pH-dependent steps the same

1981 • 118 citations

Evidence for processivity and two-step binding of the RNA substrate from studies of J1/2 mutants of the Tetrahymena ribozyme

1992 • 111 citations

Comparison of binding of mixed ribose-deoxyribose analogs of CUCU to a ribozyme and to GGAGAA by equilibrium dialysis: evidence for ribozyme specific interactions with 2'-hydroxy groups

1991 • 108 citations

RNA catalysis by a group I ribozyme. Developing a model for transition state stabilization.

1992 • 108 citations

The importance of being ribose at the cleavage site in the Tetrahymena ribozyme reaction

1993 • 105 citations

Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozyme

1993 • 102 citations

Ribozyme inhibitors. Deoxyguanosine and dideoxyguanosine are competitive inhibitors of self-splicing of the Tetrahymena ribosomal ribonucleic acid precursor

1986 • 101 citations

Group I Intron Self-Splicing with Adenosine: Evidence for a Single Nucleoside-Binding Site

1991 • 100 citations

pH Variation of isotope effects in enzyme-catalyzed reactions. 2. Isotope-dependent step not pH dependent. Kinetic mechanism of alcohol dehydrogenase

1981 • 100 citations

Stereochemical Course of Catalysis by the Tetrahymena Ribozyme

1989 • 99 citations

Contributions of 2'-hydroxyl groups of the RNA substrate to binding and catalysis by the Tetrahymena ribozyme. An energetic picture of an active site composed of RNA

1993 • 97 citations

Catalysis of the hydrolysis of phosphorylated pyridines by Mg(OH)+: a possible model for enzymic phosphoryl transfer

1990 • 94 citations

A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.

1980 • 73 citations

Guanosine binding to the Tetrahymena ribozyme: thermodynamic coupling with oligonucleotide binding.

1993 • 71 citations

Mechanism of the .alpha.-chymotrypsin-catalyzed hydrolysis of amides. pH dependence of kc and km. kinetic detection of an intermediate

1971 • 68 citations

Guanosine binding required for cyclization of the self-splicing intervening sequence ribonucleic acid from Tetrahymena thermophila

1987 • 54 citations

Binding of guanosine and 3' splice site analogs to a Group I ribozyme: Interactions with functional groups of guanosine and with additional nucleotides

1993 • 42 citations

Cooperative and anticooperative binding to a ribozyme.

1993 • 31 citations

Reduction of the potent DNA polymerase III holoenzyme 3'.fwdarw.5' exonuclease activity by template-primer analogues

1990 • 21 citations

Substrate specificity and stereospecificity of calf spleen phosphodiesterase towards deoxyribonucleosidyl 3′‐(4‐nitrophenyl phosphates) and phosphorothioates

1985 • 10 citations