Direct incorporation of guanosine 5-diphosphate into microtubules without guanosine 5'-triphosphate hydrolysis

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Colchicine-binding protein of mammalian brain and its relation to microtubules

1968 • 1,140 citations

Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules

1974 • 814 citations

A theory of linear and helical aggregations of macromolecules

1962 • 746 citations

Nucleated Assembly of Microtubules in Porcine Brain Extracts

1972 • 309 citations

Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules

1976 • 281 citations

Implications of treadmilling for the stability and polarity of actin and tubulin polymers in vivo.

1980 • 232 citations

Separation of active tubulin and microtubule-associated proteins by ultracentrifugation and isolation of a component causing the formation of microtubule bundles

1984 • 224 citations

Microtubules from Mammalian Brain: Some Properties of Their Depolymerization Products and a Proposed Mechanism of Assembly and Disassembly

1974 • 200 citations

Glutamate-induced polymerization of tubulin: Characteristics of the reaction and application to the large-scale purification of tubulin

1981 • 181 citations

Kinetic analysis of cooperativity in tubulin polymerization in the presence of guanosine di- or triphosphate nucleotides

1978 • 177 citations

Effect of antimitotic drugs on tubulin GTPase activity and self-assembly.

1979 • 142 citations

Assembly of microtubules from preformed, ring‐shaped protofilaments and 6‐s tubulin

1974 • 136 citations

The microtubule-associated nucleoside diphosphate kinase.

1984 • 120 citations

Head-to-tail polymerization of microtubules in vitro

1981 • 115 citations

Phase changes at the end of a microtubule with a GTP cap.

1984 • 96 citations

X-ray kinetic studies of microtubule assembly using synchrotron radiation

1980 • 93 citations

Stoichiometry and role of GTP hydrolysis in bovine neurotubule assembly

1978 • 89 citations

Equilibrium and kinetic analysis of microtubule assembly in the presence of guanosine diphosphate

1980 • 67 citations

Role of GTP hydrolysis in microtubule treadmilling and assembly.

1981 • 64 citations

Effect of guanosine diphosphate on microtubule assembly and stability.

1980 • 64 citations

Assembly of microtubule protein: role of guanosine di- and triphosphate nucleotides

1982 • 58 citations

Role of GTP in the Assembly of Microtubules

1977 • 56 citations

Role of co-operative interactions, microtubule-associated proteins and guanosine triphosphate in microtubule assembly: a model.

1980 • 55 citations

Maytansine inhibits nucleotide binding at the exchangeable site of tubulin

1985 • 53 citations

Participation of guanine nucleotides in nucleation and elongation steps of microtubule assembly.

1979 • 51 citations

Stoichiometry for guanine nucleotide binding to tubulin under polymerizing and nonpolymerizing conditions

1980 • 41 citations

Assembly and disassembly properties of microtubules formed in the presence of GTP, 5‘-guanylyl imidodiphosphate, and 5‘-guanylyl methylenediphosphate.

1980 • 41 citations

Deoxyguanosine nucleotide analogs: potent stimulators of microtubule nucleation with reduced affinity for the exchangeable nucleotide site of tubulin

1984 • 38 citations

Nucleoside diphosphate kinase from brain

1984 • 37 citations

Nucleotides Bound to Brain Tubulin and Reconstituted Microtubules

1974 • 31 citations

Differential effects of magnesium on tubulin-nucleotide interactions

1985 • 30 citations

Directed elongation model for microtubule GTP hydrolysis.

1985 • 28 citations

Effects of pH on tubulin-nucleotide interactions

1986 • 27 citations

The assembly of microtubule protein in vitro. The kinetic role in microtubule elongation of oligomeric fragments containing microtubule-associated proteins

1985 • 25 citations

Mechanism of tubulin assembly: GTP hydrolysis decreases the rate of microtubule depolymerization

1982 • 25 citations

Tubulin polymerization with ATP is mediated through the exchangeable GTP site

1986 • 23 citations

Direct incorporation of microtubule oligomers at high GTP concentrations

1984 • 19 citations

An isoenergetic exchange mechanism which accounts for tubulin-GDP stabilization of microtubules.

1981 • 18 citations

Assembly of microtubules from nucleotide-depleted tubulin

1985 • 13 citations

Incorporation of GDP-tubulin during elongation of microtubules invitro

1985 • 11 citations

Evidence against tubulin oligomer dissociation to tubulin dimer at assembly temperatures

1982 • 5 citations