Tubulin polymerization with ATP is mediated through the exchangeable GTP site

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Measurement of protein-binding phenomena by gel filtration

1962 • 1,275 citations

Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules

1974 • 814 citations

Separation of active tubulin and microtubule-associated proteins by ultracentrifugation and isolation of a component causing the formation of microtubule bundles

1984 • 224 citations

Kinetic analysis of cooperativity in tubulin polymerization in the presence of guanosine di- or triphosphate nucleotides

1978 • 177 citations

Nucleotide binding and phosphorylation in microtubule assembly in vitro

1977 • 163 citations

Regulation of the microtubule steady state in vitro by ATP

1979 • 99 citations

Use of a GTP photoaffinity probe to resolve aspects of the mechanism of tubulin polymerization.

1979 • 80 citations

Effects of inhibitors of tubulin polymerization on GTP hydrolysis.

1981 • 75 citations

Equilibrium and kinetic analysis of microtubule assembly in the presence of guanosine diphosphate

1980 • 67 citations

Purification of Tubulin from Bovine Brain and Its Interaction with Guanine Nucleotides

1975 • 63 citations

Direct photoaffinity labeling of tubulin with guanosine 5'-triphosphate

1985 • 60 citations

Guanosine 5'-O-(3-thiotriphosphate), a potent nucleotide inhibitor of microtubule assembly.

1984 • 59 citations

Binding of ATP to tubulin

1982 • 48 citations

Nucleotide specificity in microtubule assembly in vitro

1978 • 48 citations

Deoxyguanosine nucleotide analogs: potent stimulators of microtubule nucleation with reduced affinity for the exchangeable nucleotide site of tubulin

1984 • 38 citations

ATP-induced gelation--contraction of microtubules assembled in vitro.

1984 • 31 citations

Effect of ATP on the kinetics of microtubule assembly.

1982 • 31 citations

Affinity labeling of tubulin's exchangeable guanosine 5'-triphosphate binding site

1983 • 29 citations

Modulation of tubulin-nucleotide interactions by microtubule-associated proteins: polymerization with ribose-modified analogs of guanosine 5'-triphosphate

1983 • 28 citations

Structure-function studies with derivatives of 6-benzyl-1,3-benzodioxole, a new class of synthetic compounds which inhibit tubulin polymerization and mitosis.

1985 • 26 citations

ATP-induced aggregates of tubulin rings.

1980 • 23 citations

3′,5′-cyclic AMP binds to and promotes polymerisation on platelet tubulin

1978 • 23 citations

Monoclonal antibodies localize the exchangeable GTP‐binding site in β‐ and not α‐tubulins

1985 • 20 citations

Interactions of a new antimitotic agent, NSC-181928, with purified tubulin

1982 • 17 citations

Activities of guanosine triphosphate analogues in reactions catalyzed by elongation factor Tu and initiation factor 2 of Escherichia coli

1975 • 15 citations

Localization of the ATP binding site on α-tubulin

1983 • 10 citations

Assembly of microtubules with ATP: evidence that only a fraction of the protein is assembly‐competent

1984 • 10 citations

Microtubule Assembly in the Presence of Adenosine Triphosphate

1979 • 8 citations

Affinity labeling of the exchangeable nucleotide binding site in platelet tubulin

1984 • 4 citations