Crystal structure of calpain reveals the structural basis for Ca2+-dependent protease activity and a novel mode of enzyme activation

[20] Processing of X-ray diffraction data collected in oscillation mode

1997 • 33,348 citations

PROCHECK: a program to check the stereochemical quality of protein structures

1993 • 23,689 citations

The CCP4 suite: programs for protein crystallography

1994 • 17,203 citations

Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination

1998 • 15,791 citations

NMRPipe: A multidimensional spectral processing system based on UNIX pipes

1995 • 15,623 citations

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons

1991 • 5,267 citations

[26] Raster3D: Photorealistic molecular graphics

1997 • 3,784 citations

[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods

1997 • 1,905 citations

Methods used in the structure determination of bovine mitochondrial F1 ATPase

1996 • 1,101 citations

Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A

1995 • 976 citations

C2-domains, Structure and Function of a Universal Ca2+-binding Domain

1998 • 835 citations

Structure and physiological function of calpains

1997 • 710 citations

Structure of the first C2 domain of synaptotagmin I: A novel Ca2+/phospholipid-binding fold

1995 • 680 citations

A visual protein crystallographic software system for X11/Xview

1992 • 552 citations

Structure of papain refined at 1.65 Å resolution

1984 • 508 citations

Molecular mechanisms for the conversion of zymogens to active proteolytic enzymes

1998 • 459 citations

Alignment/Phylogeny of the Papain Superfamily of Cysteine Proteases

1995 • 387 citations

Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment.

1996 • 379 citations

THE LYSOSOMAL CYSTEINE PROTEASES

1999 • 353 citations

Calpain: A Protease in Search of a Function?

1998 • 352 citations

Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?

1984 • 337 citations

Calcium‐dependent proteases: an enzyme system active at cellular membranes?

1987 • 308 citations

Proteolytic Cleavage of Human p53 by Calpain: a Potential Regulator of Protein Stability

1997 • 305 citations

Is calpain activity regulated by membranes and autolysis or by calcium and calpastatin?

1992 • 236 citations

Structure of a calpain Ca2+-binding domain reveals a novel EF-hand and Ca2+-induced conformational changes

1997 • 205 citations

Crystal structure of calcium bound domain VI of calpain at 1.9 Å resolution and its role in enzyme assembly, regulation, and inhibitor binding

1997 • 190 citations

A novel aspect of calpain activation

1998 • 176 citations

The role of pro regions in protein folding

1993 • 174 citations

Structure of rat procathepsin B: model for inhibition of cysteine protease activity by the proregion

1996 • 170 citations

Calpain: A Cytosolic Proteinase Active at the Membranes

1997 • 154 citations

Structure and Physiology of Calpain, an Enigmatic Protease

1998 • 123 citations

Autolysis, Ca2+ Requirement, and Heterodimer Stability in m-Calpain

1997 • 114 citations

Molecular and Functional Properties of a Calpain Activator Protein Specific for μ-Isoforms

1998 • 114 citations

Optimizing Shake-and-Bake for proteins

1999 • 113 citations

The tra-3 sex determination gene of Caenorhabditis elegans encodes a member of the calpain regulatory protease family.

1996 • 112 citations

The prosequence of procaricain forms an α-helical domain that prevents access to the substrate-binding cleft

1996 • 107 citations

Ca(2+)-activated neutral protease is active in the erythrocyte membrane in its nonautolyzed 80-kDa form.

1994 • 98 citations

Calpain Dissociates into Subunits in the Presence Ions

1995 • 95 citations

Development and Therapeutic Potential of Calpain Inhibitors

1996 • 91 citations

A catalytic subunit of calpain possesses full proteolytic activity

1995 • 86 citations

Structural basis for specificity of papain-like cysteine protease proregions toward their cognate enzymes

1998 • 74 citations

Recombinant calpain II: improved expression systems and production of a C105A active-site mutant for crystallography

1995 • 74 citations

Crystal structure of the novel aspartic proteinase zymogen proplasmepsin II from plasmodium falciparum.

1999 • 71 citations

Potential m-Calpain Substrates during Myoblast Fusion

1999 • 67 citations

Crystal structure of wild‐type human procathepsin K

1999 • 63 citations

Expression of Genes (CAPN3, SGCA, SGCB, and TTN) Involved in Progressive Muscular Dystrophies during Early Human Development

1998 • 61 citations

Structural basis of profactor D activation: from a highly flexible zymogen to a novel self-inhibited serine protease, complement factor D

1999 • 58 citations

Active site residues in m‐calpain: identification by site‐directed mutagenesis

1995 • 58 citations

The effects of truncations of the small subunit on m-calpain activity and heterodimer formation

1997 • 53 citations

Genomic Organization of MouseCapn5andCapn6Genes Confirms That They Are a Distinct Calpain Subfamily

1998 • 42 citations

Translocation and Down‐Regulation of Protein Kinase C‐α, ‐β, and ‐γ Isoforms During Ischemia‐Reperfusion in Rat Brain

1999 • 39 citations

Calpain Subunits Remain Associated during Catalysis

1996 • 38 citations

Capn7: A highly divergent vertebrate calpain with a novel C-terminal domain

1999 • 37 citations

m‐Calpain subunits remain associated in the presence of calcium

1998 • 26 citations

Primary sequences of rat μ-calpain large and small subunits are, respectively, moderately and highly similar to those of human

1996 • 23 citations

Crystallization and X-ray crystallographic analysis of m-calpain, a Ca²⁺-dependent protease

1999 • 19 citations

Interaction of aspartic acid-104 and proline-287 with the active site of m-calpain

1996 • 9 citations