Comparative analysis of lymphocyte activation marker expression and cytokine secretion profile in stimulated human peripheral blood mononuclear cell cultures: an in vitro model to monitor cellular immune function

Isolation of mononuclear cells and granulocytes from human blood.

1968 • 9,672 citations

The Staphylococcal Enterotoxins and Their Relatives

1990 • 1,914 citations

Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester

1985 • 753 citations

Role of the CD28 receptor in T-cell activation

1990 • 684 citations

CD28 activation pathway regulates the production of multiple T-cell-derived lymphokines/cytokines.

1989 • 662 citations

Role of cytokines in determining T-lymphocyte function

1994 • 396 citations

Mechanisms of interleukin‐10‐mediated immune suppression

2001 • 368 citations

Leu 23 induction as an early marker of functional CD3/T cell antigen receptor triggering. Requirement for receptor cross-linking, prolonged elevation of intracellular [Ca++] and stimulation of protein kinase C.

1989 • 226 citations

Role of pro- and anti-inflammatory cytokines during inflammation: experimental and clinical findings.

1997 • 206 citations

Rapid flow cytometric method for measuring lymphocyte subset activation

1995 • 202 citations

Mechanisms of T cell activation by the calcium ionophore ionomycin.

1989 • 200 citations

Activated lymphocytes during acute Epstein-Barr virus infection.

1987 • 178 citations

Activation of human T cells by FcR nonbinding anti-CD3 mAb, hOKT3γ1(Ala-Ala)

2003 • 177 citations

Assays for monitoring cellular immune responses to active immunotherapy of cancer.

2001 • 167 citations

CD69, HLA-DR and the IL-2R Identify Persistently Activated T Cells in Psoriasis Vulgaris Lesional Skin: Blood and Skin Comparisons by Flow Cytometry

2000 • 160 citations

Contrasting Impacts of Immunosuppressive Agents (Rapamycin, FK506, Cyclosporin A, and Dexamethasone) on Bidirectional Dendritic Cell-T Cell Interaction During Antigen Presentation

2002 • 129 citations

Changes in Activated T Cells in the Blood Correlate With Disease Activity in Multiple Sclerosis

2000 • 109 citations

Transferrin synthesis by inducer T lymphocytes.

1986 • 107 citations

Alterations in levels of CD28-/CD8+ suppressor cell precursor and CD45RO+/CD4+ memory T lymphocytes in the peripheral blood of multiple sclerosis patients

1995 • 88 citations

Lectins and superantigens: Membrane interactions of these compounds with T lymphocytes affect immune responses

1993 • 83 citations

Restricted expression of p55 interleukin 2 receptor (CD25) on normal T cells

1990 • 79 citations

Validation and comparative analysis of a multiplexed assay for the simultaneous quantitative measurement of Th1/Th2 cytokines in human serum and human peripheral blood mononuclear cell culture supernatants

2004 • 78 citations

Phenotype and frequency of cells secreting IL-2, IL-4, IL-6, IL-10, IFN and TNF-α in human peripheral blood

1995 • 78 citations

Transferrin receptor induces tyrosine phosphorylation in T cells and is physically associated with the TCR zeta-chain.

1995 • 75 citations

Kinetics of Cytokine Release and Expression of Lymphocyte Cell‐Surface Activation Markers After In Vitro Stimulation of Human Peripheral Blood Mononuclear Cells with Streptococcus pneumoniae

1999 • 50 citations

Cytokine and cytokine receptor expression as a biological indicator of immune activation: important considerations in the development of in vitro model systems

2000 • 48 citations

Flow cytometric determination of estrogen receptors in intact cells.

1984 • 27 citations

Cytokine secretion induced by superantigens in peripheral blood mononuclear cells, lamina propria lymphocytes, and intraepithelial lymphocytes

1995 • 25 citations

DIFFERENTIAL REGULATION BY DEXAMETHASONE AND CYCLOSPORINE OF HUMAN T CELLS ACTIVATED BY VARIOUS STIMULI

1991 • 22 citations

Induction of responsiveness in superantigen-induced anergic T cells. Role of ligand density and costimulatory signals.

1995 • 20 citations

Stable polarization of peripheral blood T cells towards type 1 or type 2 phenotype after polyclonal activation

1998 • 15 citations

Subpopulations of T lymphocytes in psoriasis patients and their changes during immunotherapy

1987 • 12 citations

Mitogen-induced T-cell CD69 expression is a less sensitive measure of T-cell function than [3H]-thymidine uptake

1999 • 12 citations

Treatment of acute kidney allograft rejection with a non-mitogenic CD3 antibody

2003 • 12 citations

Distribution and Function of Lymphocyte Surface Antigens

1993 • 11 citations