Biophysical Properties of Human Antibody Variable Domains

MOLMOL: A program for display and analysis of macromolecular structures

1996 • 7,169 citations

Sequences of proteins of immunological interest

1984 • 3,427 citations

Protein structure: A practical approach

1990 • 2,071 citations

Humanization of an anti-p185HER2 antibody for human cancer therapy.

1992 • 1,867 citations

Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding

1995 • 1,706 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

Protein aggregation: folding aggregates, inclusion bodies and amyloid

1998 • 1,118 citations

Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library

1996 • 1,021 citations

Isolation of high affinity human antibodies directly from large synthetic repertoires.

1994 • 970 citations

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides 1 1Edited by I. A. Wilson

2000 • 722 citations

The repertoire of human germline vH sequences reveals about fifty groups of VH segments with different hypervariable loops

1992 • 616 citations

Stability engineering of antibody single-chain Fv fragments

2001 • 587 citations

Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme

1996 • 490 citations

Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

1997 • 485 citations

Measuring and increasing protein stability

1990 • 470 citations

A comparison of strategies to stabilize immunoglobulin Fv-fragments

1990 • 469 citations

Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies

1999 • 427 citations

Design and Use of a Phage Display Library

1998 • 423 citations

Renaturation, Purification and Characterization of Recombinant Fab-Fragments Produced in Escherichia coli

1991 • 385 citations

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display

2000 • 372 citations

Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries

2000 • 330 citations

Roles of electrostatic interaction in proteins

1996 • 300 citations

Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment

1997 • 241 citations

Engineered turns of a recombinant antibody improve its in vivo folding

1995 • 238 citations

Yet Another Numbering Scheme for Immunoglobulin Variable Domains: An Automatic Modeling and Analysis Tool

2001 • 224 citations

High thermal stability is essential for tumor targeting of antibody fragments: engineering of a humanized anti-epithelial glycoprotein-2 (epithelial cell adhesion molecule) single-chain Fv fragment.

1999 • 202 citations

Selection for a periplasmic factor improving phage display and functional periplasmic expression

1998 • 201 citations

Selection for improved protein stability by phage display 1 1Edited by J. A. Wells

1999 • 199 citations

The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli

1992 • 190 citations

X-ray Structures of the Antigen-binding Domains from Three Variants of Humanized anti-p185HER2 Antibody 4D5 and Comparison with Molecular Modeling

1993 • 185 citations

Biophysical Properties of Camelid V_HH Domains Compared to Those of Human V_H3 Domains

2002 • 173 citations

A single-domain antibody fragment in complex with RNase A: non-canonical loop structures and nanomolar affinity using two CDR loops

1999 • 160 citations

Physicochemical consequences of amino acid variations that contribute to fibril formation by immunoglobulin light chains

1999 • 159 citations

Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated R1pA

1996 • 147 citations

The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies

2002 • 135 citations

Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors

2000 • 134 citations

Different Equilibrium Stability Behavior of ScFv Fragments: Identification, Classification, and Improvement by Protein Engineering

1999 • 132 citations

Recombinant antibody fragments

1998 • 129 citations

Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity

1997 • 126 citations

By-passing selection: direct screening for antibody-antigen interactions using protein arrays

2000 • 122 citations

Specific Detection of His-Tagged Proteins with Recombinant Anti-His Tag scFv-Phosphatase or scFv-Phage Fusions

1997 • 107 citations

ANTIBODY ENGINEERING

1997 • 105 citations

Structure-Based Improvement of the Biophysical Properties of Immunoglobulin V_H Domains with a Generalizable Approach

2003 • 101 citations

Four structural risk factors identify most fibril-forming kappa light chains

2000 • 98 citations

Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

2002 • 91 citations

Identification of Framework Residues in a Secreted Recombinant Antibody Fragment That Control Production Level and Localization inEscherichia coli

1997 • 76 citations

Antibody engineering — A practical approach

1997 • 72 citations

Intrabody construction and expression. I. The critical role of VL domain stability

1999 • 69 citations

Intrabody construction and expression III: Engineering hyperstable V_H domains

1999 • 68 citations

Domain interactions in antibody Fv and scFv fragments: effects on unfolding kinetics and equilibria

1999 • 66 citations

High-level temperature-induced synthesis of an antibody VH-domain in Escherichia coli using the PelB secretion signal

1992 • 66 citations

The Importance of Framework Residues H6, H7 and H10 in Antibody Heavy Chains: Experimental Evidence for a New Structural Subclassification of Antibody VH Domains

2001 • 63 citations

Exploiting antibody-based technologies to manage environmental pollution

1999 • 62 citations

The Influence of the Buried Glutamine or Glutamate Residue in Position 6 on the Structure of Immunoglobulin Variable Domains

2001 • 52 citations

Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen 1 1Edited by P. E. Wright

1999 • 50 citations

Sequence analysis and bacterial production of the anti‐c‐myc antibody 9E10: the V_H domain has an extended CDR‐H3 and exhibits unusual solubility

1997 • 49 citations

Stabilization of antibody fragments in adverse environments

1998 • 47 citations

Structural Patterns at Residue Positions 9, 18, 67 and 82 in the VH Framework Regions of Human and Murine Immunoglobulins

1993 • 39 citations

The scFv fragment of the antibody hu4d5-8: evidence for early premature domain interaction in refolding

2001 • 31 citations

Synthesis, physicochemical characterization, and crystallization of a putative retro‐coiled coil

1998 • 14 citations

Deleted Work

1955 • 0 citations