Positive-selection vectors utilizing lethality of the EcoRI endonuclease

Experiments in molecular genetics

1972 • 28,260 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322

1979 • 1,807 citations

Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

1977 • 1,252 citations

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

1977 • 711 citations

Nucleotide sequence of the kanamycin resistance transposon Tn903

1981 • 577 citations

Construction of plasmids carrying the cI gene of bacteriophage lambda.

1976 • 410 citations

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

1978 • 282 citations

Revised Interpretation of the Origin of the pSC101 Plasmid

1977 • 188 citations

DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

1981 • 167 citations

An improved positive selection plasmid vector constructed by ollgonucleotide mediated mutagenesis

1983 • 156 citations

A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.

1976 • 137 citations

A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids

1981 • 109 citations

A plasmid cloning vehicle allowing a positive selection for inserted fragments

1980 • 105 citations

A novel cloning vector for the direct selection of recombinant DNA in E. coli

1982 • 97 citations

Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli

1977 • 74 citations

Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors

1982 • 73 citations

Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants

1982 • 70 citations

Lethality of palindromic DNA and its use in selection of recombinant plasmids

1982 • 62 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

Transposable elements of Halobacterium halobium

1983 • 39 citations

A novel ColEl::Tn3 plasmid vector that allows direct selection of hybrid clones in E. coli

1980 • 34 citations

Plasmid vectors for positive selection of DNA inserts controlled by the λ pl promoter, repressor and antitermination function

1981 • 26 citations

The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434

1984 • 26 citations

Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli

1984 • 26 citations

Positive selection vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments

1984 • 23 citations

Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids

1979 • 21 citations

Plasmid vectors derived from phage Mu allow direct selection of transformants containing cloned HindIII and PstI fragments

1980 • 11 citations