Design and Validation of an Enzyme-Linked Immunospot Assay for Use in Clinical Trials of Candidate HIV Vaccines

HIV-specific cytotoxic T lymphocytes in seropositive individuals

1987 • 797 citations

Rapid Effector Function in CD8+ Memory T Cells

1997 • 652 citations

A Re-Evaluation of the Frequency of CD8+ T Cells Specific for EBV in Healthy Virus Carriers

1999 • 520 citations

HIV-1-Specific Mucosal CD8+ Lymphocyte Responses in the Cervix of HIV-1-Resistant Prostitutes in Nairobi

2000 • 379 citations

Quantification of antigen specific CD8+ T cells using an ELISPOT assay

1995 • 367 citations

Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime

1998 • 227 citations

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

1991 • 226 citations

Phase I trial of a MART-1 peptide vaccine with incomplete Freund's adjuvant for resected high-risk melanoma.

1999 • 223 citations

Identification of functional subsets by flow cytometry: Intracellular detection of cytokine expression

1998 • 219 citations

Design and construction of an experimental HIV-1 vaccine for a year-2000 clinical trial in Kenya.

2000 • 199 citations

The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected individuals

2001 • 195 citations

Development and Validation of a Gamma Interferon ELISPOT Assay for Quantitation of Cellular Immune Responses to Varicella-Zoster Virus

2001 • 177 citations

A sensitive ELISPOT assay to detect low-frequency human T lymphocytes

1997 • 170 citations

Accelerating the development and future availability of HIV-1 vaccines: why, when, where, and how?

2000 • 168 citations

DNA multi-CTL epitope vaccines for HIV and Plasmodium falciparum: immunogenicity in mice

1998 • 140 citations

Quantitation of antigen-reactive T cells in peripheral blood by IFNγ-ELISPOT assay and chromium-release assay: a four-centre comparative trial

2000 • 135 citations

Functionally Inert HIV-Specific Cytotoxic T Lymphocytes Do Not Play a Major Role in Chronically Infected Adults and Children

2000 • 127 citations

Preservation of Lymphocyte Immunophenotype and Proliferative Responses in Cryopreserved Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus Type 1-Infected Donors: Implications for Multicenter Clinical Trials

2000 • 126 citations

Optimization of methods to assess human mucosal T-cell responses to HIV infection

2003 • 107 citations

Reverse enzyme-linked immunospot assay (RELISPOT) for the detection of cells secreting immunoreactive substances

1984 • 90 citations

Immunogenicities of intravenous and intramuscular administrations of modified vaccinia virus Ankara-based multi-CTL epitope vaccine for human immunodeficiency virus type 1 in mice.

1998 • 84 citations

Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse transcriptase.

1992 • 81 citations

A DNA/MVA-based candidate human immunodeficiency virus vaccine for Kenya induces multi-specific T cell responses in rhesus macaques

2002 • 79 citations

Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15—the Amplispot assay

2002 • 69 citations

Detection of antigen-specific T cell interferon γ expression by ELISPOT and cytokine flow cytometry assays in rhesus macaques

2003 • 60 citations

Quantitation of human influenza virus-specific cytotoxic T lymphocytes: correlation of cytotoxicity and increased numbers of IFN-γ producing CD8⁺ T cells

1994 • 50 citations

CD8⁺Cytotoxic T Lymphocyte Responses to Lytic Proteins of Human Herpes Virus 8 in Human Immunodeficiency Virus Type 1–Infected and –Uninfected Individuals

2000 • 49 citations

Identification of multiple HIV-1 CTL epitopes presented by HLA-B∗5101 molecules

1999 • 48 citations

Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli

2000 • 45 citations

ELISPOT assay for detection of peptide specific Interferon-γ secreting cells in rhesus macaques

2001 • 45 citations

A review of vaccines for HIV prevention

2002 • 33 citations

Effect of Cryopreservation on Measurement of Cytomegalovirus-Specific Cellular Immune Responses in HIV-Infected Patients

2000 • 30 citations

Effects of Cryopreservation on Immune Responses

1995 • 21 citations

Comparative studies on in vitro reactivity of fresh and cryopreserved pig lymphocytes

1991 • 20 citations

Human class I MHC-restricted cytotoxic T-lymphocytes specific for human immunodeficiency virus envelope antigens

1988 • 15 citations

Recombinant modified vaccinia virus Ankara efficiently restimulates human cytotoxic T lymphocytes in vitro

2000 • 12 citations

Prospect of a prophylactic vaccine for HIV

2001 • 12 citations

Effects of Cryopreservation on Immune Responses

1996 • 10 citations