Reconstruction by site-directed mutagenesis of the transition state for the activation of tyrosine by the tyrosyl-tRNA synthetase: a mobile loop envelopes the transition state in an induced-fit mechanism

The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

1984 • 563 citations

Improved oligonudeotide site-directed rautagenesis using M13 vectors

1985 • 545 citations

Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding

1982 • 348 citations

Site-directed mutagenesis as a probe of enzyme structure and catalysis: tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation

1983 • 268 citations

Transition-state stabilization in the mechanism of tyrosyl-tRNA synthetase revealed by protein engineering.

1985 • 179 citations

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering

1987 • 167 citations

Structure-activity relationships in engineered proteins: analysis of use of binding energy by linear free energy relationships

1987 • 154 citations

A large increase in enzyme–substrate affinity by protein engineering

1984 • 147 citations

Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine

1987 • 117 citations

Demonstration of two active sites on a monomeric aminoacyl-tRNA synthetase. Possible roles of negative cooperativity and half-of-the-sites reactivity in oligomeric enzymes

1975 • 117 citations

A model of synthetase/transfer RNA interaction as deduced by protein engineering

1986 • 117 citations

Use of binding energy in catalysis analyzed by mutagenesis of the tyrosyl-tRNA synthetase

1986 • 116 citations

The Amino Acid Sequence of the Tyrosy1-tRNA Synthetase from Bacillus stearothermophilus

1983 • 112 citations

Quantitative analysis of structure–activity relationships in engineered proteins by linear free-energy relationships

1986 • 100 citations

Probing histidine-substrate interactions in tyrosyl-tRNA synthetase using asparagine and glutamine replacements

1985 • 88 citations

Ligand binding and enzymic catalysis coupled through subunits in tyrosyl-tRNA synthetase

1975 • 88 citations

Binding energy and catalysis: a lesson from protein engineering of the tyrosyl-tRNA synthetase

1986 • 76 citations

Conserved cysteine and histidine residues in the structures of the tyrosyl and methionyl‐tRNA synthetases

1982 • 45 citations

Internal thermodynamics of position 51 mutants and natural variants of tyrosyl-tRNA synthetase

1986 • 39 citations

Investigation of transition-state stabilization by residues histidine-45 and threonine-40 in the tyrosyl-tRNA synthetase

1987 • 38 citations

Structure of a mutant of tyrosyl-tRNA synthetase with enhanced catalytic properties

1987 • 29 citations

Free energy of hydrolysis of tyrosyl adenylate and its binding to wild-type and engineered mutant tyrosyl-tRNA synthetases

1986 • 27 citations

A transcription terminator in the 5′ non‐coding region of the tyrosyl tRNA synthetase gene from Bacillus stearothermophilus

1986 • 14 citations