Phosphorylation of Both Phosphoacceptor Sites in the HIV-1 Vpu Cytoplasmic Domain Is Essential for Vpu-Mediated ER Degradation of CD4

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus

1984 • 3,530 citations

Protein modules and signalling networks

1995 • 2,447 citations

T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV

1984 • 2,441 citations

Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells

1994 • 1,999 citations

Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4

1992 • 553 citations

The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release

1990 • 461 citations

A Novel Gene of HIV-1, vpu , and Its 16-Kilodalton Product

1988 • 445 citations

Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

1987 • 409 citations

Identification of a protein encoded by the vpu gene of HIV-1

1988 • 295 citations

Functional role of human immunodeficiency virus type 1 vpu.

1989 • 280 citations

The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains

1996 • 276 citations

Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein

1989 • 267 citations

Human immunodeficiency virus type 1 Vpu protein regulates the formation of intracellular gp160-CD4 complexes

1992 • 251 citations

Env and Vpu proteins of human immunodeficiency virus type 1 are produced from multiple bicistronic mRNAs

1990 • 250 citations

CD4 is retained in the endoplasmic reticulum by the human immunodeficiency virus type 1 glycoprotein precursor

1990 • 228 citations

CD4 down-modulation during infection of human T cells with human immunodeficiency virus type 1 involves independent activities of vpu, env, and nef

1996 • 227 citations

Human immunodeficiency virus type 1 Vpu protein is an oligomeric type I integral membrane protein

1993 • 218 citations

Differential activities of the human immunodeficiency virus type 1-encoded Vpu protein are regulated by phosphorylation and occur in different cellular compartments

1994 • 201 citations

Vpu protein of human immunodeficiency virus type 1 enhances the release of capsids produced by gag gene constructs of widely divergent retroviruses.

1993 • 189 citations

The human immunodeficiency virus type 1 Vpu protein specifically binds to the cytoplasmic domain of CD4: implications for the mechanism of degradation

1995 • 188 citations

The Human Immunodeficiency Virus Type 1 Encoded Vpu Protein is Phosphorylated by Casein Kinase-2 (CK-2) at Positions Ser52 and Ser56 within a Predicted α-Helix-Turn-α-Helix-Motif

1994 • 148 citations

The human immunodeficiency virus type 1 (HIV-1) CD4 receptor and its central role in promotion of HIV-1 infection

1995 • 142 citations

Human immunodeficiency virus type 1 Vpu protein induces degradation of CD4 in vitro: the cytoplasmic domain of CD4 contributes to Vpu sensitivity

1993 • 134 citations

Intracellular interaction of human immunodeficiency virus type 1 (ARV-2) envelope glycoprotein gp160 with CD4 blocks the movement and maturation of CD4 to the plasma membrane

1990 • 131 citations

Vpu-induced degradation of CD4: requirement for specific amino acid residues in the cytoplasmic domain of CD4

1993 • 91 citations

Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain in Vpu-induced degradation in the endoplasmic reticulum

1993 • 81 citations

Envelope glycoprotein and CD4 independence of vpu-facilitated human immunodeficiency virus type 1 capsid export

1992 • 78 citations

Functional Analysis of the Phosphorylation Sites on the Human Immunodeficiency Virus Type 1 Vpu Protein

1995 • 60 citations

Depletion of the surface CD4 molecule by the envelope protein of human immunodeficiency virus expressed in a human CD4+ monocytoid cell line

1989 • 60 citations

Cell type‐dependence for Vpu function

1994 • 50 citations

Sequences present in the cytoplasmic domain of CD4 are necessary and sufficient to confer sensitivity to the human immunodeficiency virus type 1 Vpu protein

1994 • 50 citations

Degradation of CD4 Induced by Human Immunodeficiency Virus Type 1 Vpu Protein: A Predicted Alpha-Helix Structure in the Proximal Cytoplasmic Region of CD4 Contributes to Vpu Sensitivity

1995 • 48 citations

Human immunodeficiency virus type 1 Vpu has a CD4- and an envelope glycoprotein-independent function

1993 • 39 citations

The Human Immunodeficiency Virus Type 1 Vpu Protein: A Potential Regulator of Proteolysis and Protein Transport in the Mammalian Secretory Pathway

1995 • 34 citations

Vpu-Mediated Proteolysis of gp160/CD4 Chimeric Envelope Glycoproteins in the Endoplasmic Reticulum: Requirement of Both the Anchor and Cytoplasmic Domains of CD4

1994 • 27 citations

Stimulation of Heterologous Protein Degradation by the Vpu Protein of HIV-1 Requires the Transmembrane and Cytoplasmic Domains of CD4

1994 • 26 citations

The Human Immunodeficiency Virus Type 1 Vpu Protein Tethered to the CD4 Extracellular Domain Is Localized to the Plasma Membrane and Is Biologically Active in the Secretory Pathway of Mammalian Cells: Implications for the Mechanisms of Vpu Function

1996 • 18 citations

The Human Immunodeficiency Virus Type 1 Vpu Protein: Roles in Virus Release and CD4 Downregulation

1995 • 15 citations

Analysis of endoproteolytic cleavage and intracellular transport of human immunodeficiency virus type 1 envelope glycoproteins using mutant CD4 molecules bearing the transmembrane endoplasmic reticulum retention signal

1993 • 14 citations