Isolation of the plasma membrane and organelles from Chinese hamster ovary cells

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 • 47,127 citations

Amino Acid Metabolism in Mammalian Cell Cultures

1959 • 4,025 citations

Form and function of phospholipids

1973 • 1,072 citations

Transport of cholesterol from the endoplasmic reticulum to the plasma membrane.

1985 • 263 citations

Lateral diffusion in phospholipid multibilayers measured by fluorescence recovery after photobleaching

1977 • 258 citations

Action of highly purified phospholipases on blood platelets. Evidence for an asymmetric distribution of phospholipids in the surface membrane

1977 • 248 citations

[40] Preparations of succinate—cytochrome c reductase and the cytochrome b-c1 particle, and reconstitution of succinate-cytochrome c reductase

1967 • 223 citations

Isolation and characterization of Chinese hamster ovary cell mutants defective in assembly of peroxisomes.

1990 • 213 citations

Intracellular transport of cholesterol to the plasma membrane.

1982 • 203 citations

[22] Preparation of plasma-membrane fragments from mouse ascites tumor cells

1966 • 198 citations

Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins.

1983 • 185 citations

Mutants of Chinese hamster ovary cells with altered membrane phospholipid composition. Replacement of phosphatidylinositol by phosphatidylglycerol in a myo-inositol auxotroph.

1980 • 174 citations

Characterization of serine palmitoyltransferase activity in Chinese hamster ovary cells

1983 • 151 citations

Intracellular transport of phosphatidylcholine to the plasma membrane.

1985 • 145 citations

Assignment of methylene proton resonances in NMR spectra of embryonic and transformed cells to plasma membrane triglyceride.

1986 • 145 citations

Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets A determination using purified phospholipases and a rapid method for membrane isolation

1979 • 144 citations

A critical evaluation of plasma membrane fractionation

1973 • 125 citations

Fluorescence recovery after photobleaching (FRAP) experiments under conditions of uniform disk illumination. Critical comparison of analytical solutions, and a new mathematical method for calculation of diffusion coefficient D

1988 • 101 citations

Isolation and characterization of Chinese hamster ovary cells defective in the intracellular metabolism of low density lipoprotein-derived cholesterol.

1992 • 97 citations

Analysis of phospholipase C (Bacillus cereus) action toward mixed micelles of phospholipid and surfactant

1976 • 75 citations

Volume regulation of chinese hamster ovary cells in anisoosmotic media

1984 • 65 citations

Differential activation by fMet‐Leu‐Phe and phorbol ester of a plasma membrane phosphatidylcholine‐specific phospholipase D in human neutrophil

1989 • 63 citations

Lysosomal origin of the ferric iron required for cell killing by hydrogen peroxide

1985 • 63 citations

Isolation of somatic cell mutants defective in the biosynthesis of phosphatidylethanolamine.

1981 • 59 citations

Chinese hamster ovary cells deficient in peroxisomes lack the nonspecific lipid transfer protein (sterol carrier protein 2).

1990 • 58 citations

Transverse and lateral distribution of phospholipids and glycolipids in the membrane of the bacterium Micrococcus luteus

1989 • 57 citations

Biochemical characterization of plasma membranes and intracellular membranes isolated from human platelets using Percoll gradients

1986 • 56 citations

Membrane lipid modification of chinese hamster ovary cells. Thermal properties of membrane phospholipids.

1978 • 55 citations

Isolation and characterization of plasma membranes from Krebs II ascite cells using percoll gradient

1982 • 55 citations

Studies on enzymes related to diacylglycerol production in activated platelets II. Subcellular distribution, enzymatic properties and positional specificity of diacylglycerol- and monoacylglycerol-lipases

1984 • 44 citations

A rapid isolation procedure of plasma membranes from human neutrophils using self-generating Percoll gradients. Importance of pH in avoiding contamination by intracellular membranes

1985 • 38 citations

Synthesis and physical properties of phosphatidylcholine labelled with 9-(2-anthryl)nonanoic acid, a new fluorescent probe

1983 • 36 citations

Comparative study of the lateral motion of extrinsic probes and anthracene‐labelled constitutive phospholipids in the plasma membrane of Chinese hamster ovary cells

1988 • 31 citations

Evidence for the incorporation of a fluorescent anthracene fatty acid into the membrane lipids of Micrococcus luteus

1982 • 27 citations

Steady‐state and time‐resolved fluorescence anisotropy study of phospholipid molecular motion in the gel phase using 1‐palmitoyl‐2[9‐(2‐anthryl)‐nonanoyl]‐sn‐glycero‐3‐phosphocholine as probe

1985 • 27 citations

Photo‐induced dimerization of anthracene phospholipids for the study of the lateral distribution of lipids in membranes

1984 • 24 citations

Characterization of plasma membranes from A431 cells, isolated by self-generating Percoll gradient: a rapid isolation procedure to obtain plasma membranes with functional epidermal growth factor receptors

1988 • 23 citations

Isolation of the dorsal, ventral and intracellular domains of HeLa cell plasma membranes following adhesion to a gelatin substrate

1985 • 22 citations

Metabolic incorporation of 9-(2-anthryl)-nonanoic acid, a new fluorescent and photoactivable probe, into the membrane lipids of Chinese hamster ovary cells

1986 • 22 citations

Cell substrate adhesion-induced redistribution of proteins among the apical, basal, and internal domains of the plasma membrane of HeLa cells spreading on gelatin.

1987 • 18 citations

Evidence for a homogeneous lateral distribution of lipids in a bacterial membrane

1984 • 17 citations

Purification and characterization of lysosomes from Chinese hamster ovary cells

1987 • 16 citations

Continuous fluorescence microphotolysis of anthracene-labeled phospholipids in membranes. Theoretical approach of the simultaneous determination of their photodimerization and lateral diffusion rates

1989 • 11 citations

Plasma membrane isolated with a defined orientation used to investigate protein topography

1986 • 10 citations

Characterization of a Chinese Hamster Ovary Cell Line Resistant to Uncouplers

1983 • 8 citations

Isolation of plasma membrane from human blood monocytes

1985 • 6 citations

Assessment of enzyme inhibition or stimulation in plasma membrane preparations

1988 • 4 citations