High–Level Secretion and Very Efficient Isotopic Labeling of Tick Anticoagulant Peptide (TAP) Expressed in the Methylotrophic Yeast, Pichia pastoris

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Foreign gene expression in yeast: a review

1992 • 1,039 citations

Recent Advances in the Expression of Foreign Genes in Pichia pastoris

1993 • 919 citations

Pichia pastoris as a Host System for Transformations

1985 • 495 citations

Pichia pastoris as a host system for transformations.

1985 • 423 citations

Tick Anticoagulant Peptide (TAP) Is a Novel Inhibitor of Blood Coagulation Factor Xa

1990 • 414 citations

High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene

1991 • 398 citations

Expression of thelacZgene from two methanol-regulated promoters inPichia pastoris

1987 • 364 citations

High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris

1987 • 332 citations

Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies

1991 • 330 citations

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers

1989 • 321 citations

Rapid Selection Using G418 of High Copy Number Transformants of Pichia pastoris for High–level Foreign Gene Expression

1994 • 308 citations

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

1985 • 271 citations

High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast, Pichia Pastoris

1987 • 247 citations

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris

1989 • 155 citations

Continuous Production of a Novel Lysozyme via Secretion from the Yeast, Pichia pastoris

1989 • 112 citations

Structural and Functional Characterization of Tick Anticoagulant Peptide (TAP): A Potent and Selective Inhibitor of Blood Coagulation Factor Xa

1993 • 111 citations

Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties

1991 • 105 citations

Characterization of recombinant tick anticoagulant peptide. A highly selective inhibitor of blood coagulation factor Xa.

1990 • 104 citations

High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris

1988 • 94 citations

High-level secretion of biologically active aprotinin from the yeastPichia pastoris

1991 • 81 citations

High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor

1992 • 59 citations

Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences

1991 • 53 citations

Methylotrophic yeast Pichia pastoris produced in high‐cell‐density fermentations with high cell yields as vehicle for recombinant protein production

1989 • 50 citations

The dacA gene of Bacillus stearothermophilus coding for d-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris

1993 • 43 citations

Purification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli

1993 • 41 citations

Large-scale purification and characterization of recombinant tick anticoagulant peptide

1992 • 14 citations