Cloning and analysis of cDNAs encoding plant storage protein precursors

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

1977 • 2,408 citations

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

1977 • 2,233 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Nature of Col E ₁ Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol

1972 • 1,202 citations

Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9

1977 • 1,055 citations

Legumin and vicilin, storage proteins of legume seeds

1976 • 894 citations

Micro‐sequence analysis of peptides and proteins using 4‐NN‐dimethylaminoazobenzene 4′‐isothiocyanate/phenylisothiocyanate double coupling method

1978 • 834 citations

PROCESSING MECHANISMS IN THE BIOSYNTHESIS OF PROTEINS*

1980 • 461 citations

A single mouse α-amylase gene specifies two different tissue-specific mRNAs

1981 • 402 citations

Cloning and sequence analysis of cDNAs encoding two distinct somatostatin precursors found in the endocrine pancreas of anglerfish

1980 • 337 citations

[58] DNA sequence analysis by primed synthesis

1980 • 286 citations

Sequence at the 3′ end of globin mRNA shows homology with immunoglobulin light chain mRNA

1974 • 256 citations

[53] Determination of fragment order through partial digests and multiple enzyme digests

1980 • 246 citations

A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis

1980 • 234 citations

Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase

1980 • 202 citations

Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization

1978 • 201 citations

Biochemistry of Legume Seed Proteins

1975 • 182 citations

[25] Colony hybridization

1979 • 181 citations

Insulin Today

1977 • 172 citations

The isolation of high molecular weight DNA from whole organisms or large tissue masses

1978 • 171 citations

Partial characterization of the acidic and basic polypeptides of glycinin.

1979 • 167 citations

Molecular evolution of human and rabbit beta-globin mRNAs.

1977 • 157 citations

The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.)

1980 • 155 citations

Nucleotide sequence of a cDNA clone encoding human preproinsulin

1979 • 141 citations

Characterisation and Subunit Structures of the Vicilin Storage Proteins of Pea (Pisum sativum L.)

1981 • 133 citations

Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds

1979 • 119 citations

Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.)

1980 • 116 citations

Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.)

1980 • 108 citations

Cotyledonary Storage Proteins in Pisum sativum. I. Molecular Heterogeneity

1978 • 88 citations

Isoelectric-focusing properties and carbohydrate content of pea (Pisum sativum) legumin

1980 • 84 citations

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA

1979 • 81 citations

A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants

1980 • 78 citations

Precursor Forms of Pea Vicilin Subunits

1981 • 71 citations

The Peptide Pools of Germinating Barley Grains: Relation to Hydrolysis and Transport of Storage Proteins

1981 • 68 citations

[75] Isolation of ribonucleic acids from mammalian cells and animal viruses

1967 • 64 citations

LEGUMIN OF PISUM SATIVUM AND VICIA FABA

1979 • 64 citations

Endoplasmic reticulum in developing seeds of Vicia faba

1979 • 63 citations

Cloning of chicken lysozyme structural gene sequences synthesized in vitro

1978 • 60 citations

Deoxyribonucleic Acid and Ribonucleic Acid Synthesis during the Cell Expansion Phase of Cotyledon Development in Vicia faba L

1973 • 55 citations

The 3′ terminal sequence of chicken ovalumin messenger RNA and its comparison with other messenger RNA molecules

1976 • 52 citations

Purification and characterization of covalently closed replicative intermediates of ColEl DNA from Escherichia coli

1977 • 50 citations

Cell free synthesis of some storage protein subunits by polyribosomes and RNA isolated from developing seeds of pea (Pisum sativum L.)

1979 • 48 citations

Heterogeneity in subunit composition of the legumin of Pisum sativum

1979 • 36 citations

N-terminal amino acid sequence of β-subunits of legumin from Pisum sativum

1981 • 27 citations

Immunoelectrophoretic analysis of seed proteins from Pisum sativum L.

1978 • 25 citations

Synthesis of complementary DNAs to partially purified mRNAs coding for the storage proteins of Pisum sativum (L)

1980 • 21 citations

STUDIES OF CLONED DNA ENCODING THE STRUCTURE FOR THE BOVINE CORTICOTROPIN‐ß‐LIPOTROPIN PRECURSOR PROTEIN*

1980 • 9 citations

CONTROL OF SEED STORAGE PROTEIN SYNTHESIS IN DEVELOPING PISU

1981 • 1 citations