A High-Level Mammalian Expression System Based on the Madin–Darby Canine Kidney Cell Line

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Cloning and expression of the human erythropoietin gene.

1985 • 1,144 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

Recent Advances in the Expression of Foreign Genes in Pichia pastoris

1993 • 919 citations

Production of human beta interferon in insect cells infected with a baculovirus expression vector.

1983 • 907 citations

Molecular cloning and expression of collagenase-3, a novel human matrix metalloproteinase produced by breast carcinomas

1994 • 769 citations

Furin-dependent intracellular activation of the human stromelysin-3 zymogen

1995 • 620 citations

Transmembrane-deletion Mutants of the Membrane-type Matrix Metalloproteinase-1 Process Progelatinase A and Express Intrinsic Matrix-degrading Activity

1996 • 392 citations

Cloning and expression of the hyman erythropoietin gene

1985 • 353 citations

The Role of the C-terminal Domain of Human Collagenase-3 (MMP-13) in the Activation of Procollagenase-3, Substrate Specificity, and Tissue Inhibitor of Metalloproteinase Interaction

1997 • 345 citations

Mutation of the Active Site Glutamic Acid of Human Gelatinase A: Effects on Latency, Catalysis, and the Binding of Tissue Inhibitor of Metalloproteinases-1

1994 • 67 citations

Production of Heterologous Proteins Using the Baculovirus/lnsect Expression System

2003 • 19 citations

[5] Stable high-level gene expression in mammalian cells by T7 phage RNA polymerase

1993 • 18 citations