A dominant positive and negative selectable gene for use in mammalian cells.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells

1987 • 2,368 citations

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes

1988 • 1,690 citations

Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells

1977 • 1,411 citations

Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor

1978 • 1,284 citations

Expression of a Bacterial Gene in Mammalian Cells

1980 • 788 citations

Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: Identification of an upstream control region

1981 • 646 citations

Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine

1963 • 611 citations

The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene

1980 • 556 citations

Plasmid-Determined Resistance to Antimicrobial Agents

1978 • 437 citations

Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells

1991 • 338 citations

Helper-independent transformation by unintegrated Harvey sarcoma virus DNA

1978 • 277 citations

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

1979 • 238 citations

Testing an "in-out" targeting procedure for making subtle genomic modifications in mouse embryonic stem cells.

1991 • 179 citations

Competition hybridization by “pre-saturation” of HeLa cell DNA

1969 • 167 citations

Dominant Positive and Negative Selection Using a Hygromycin Phosphotransferase-Thymidine Kinase Fusion Gene

1991 • 167 citations

Positive genetic selection for gene disruption in mammalian cells by homologous recombination.

1989 • 120 citations

Protein fusions with the kanamycin resistance gene from transposon Tn5.

1984 • 114 citations

Testing an “In-Out” Targeting Procedure for Making Subtle Genomic Modifications in Mouse Embryonic Stem Cells

1991 • 46 citations