PEGylation of Val-1(α) Destabilizes the Tetrameric Structure of Hemoglobin

Mechanisms of cooperativity and allosteric regulation in proteins

1989 • 615 citations

THE STEREOCHEMICAL MECHANISM OF THE COOPERATIVE EFFECTS IN HEMOGLOBIN REVISITED

1998 • 544 citations

Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2·5 Å resolution: Refinement of the atomic model

1975 • 431 citations

Origin of the heme Cotton effects in myoglobin and hemoglobin

1971 • 339 citations

Mono-N-terminal poly(ethylene glycol)–protein conjugates

2002 • 299 citations

Isolation and characterization of a new hemoglobin derivative cross-linked between the alpha chains (lysine 99 alpha 1----lysine 99 alpha 2).

1986 • 296 citations

Influence of globin structure on the state of the heme. I. Human deoxyhemoglobin

1974 • 288 citations

MP4, a new nonvasoactive PEG‐Hb conjugate

2003 • 254 citations

New effectors of human hemoglobin: structure and function

1990 • 129 citations

X-ray diffraction and solution studies of specifically carbamylated human hemoglobin A. Evidence for the location of a proton- and oxygen-linked chloride binding site at valine 1 alpha.

1979 • 128 citations

Hemoglobin providence. Functional consequences of two alterations of the 2,3-diphosphoglycerate binding site at position beta 82.

1976 • 119 citations

Dissociation of hemoglobin into subunits

1971 • 119 citations

Mutual effects of protons, NaCl, and oxygen on the dimer-tetramer assembly of human hemoglobin. The dimer Bohr effect.

1981 • 113 citations

Structural and Biophysical Characterization of the 40 kDa PEG−Interferon-α_2aand Its Individual Positional Isomers

2005 • 112 citations

Determination of the pK values for the alpha-amino groups of human hemoglobin.

1975 • 106 citations

Solvent regulation of oxygen affinity in hemoglobin. Sensitivity of bovine hemoglobin to chloride ions.

1984 • 106 citations

Free energy of burying hydrophobic residues in the interface between protein subunits

1998 • 92 citations

Blood substitutes

2000 • 89 citations

Conjugation of Multiple Copies of Polyethylene Glycol to Hemoglobin Facilitated Through Thiolation: Influence on Hemoglobin Structure and Function

2005 • 74 citations

Subunit dissociations in natural and recombinant hemoglobins

1996 • 72 citations

Schiff base adducts of glyceraldehyde with hemoglobin. Differences in the Amadori rearrangement at the alpha-amino groups.

1983 • 67 citations

Influence of the chemistry of conjugation of poly(ethylene glycol) to Hb on the oxygen-binding and solution properties of the PEG-Hb conjugate

2005 • 63 citations

Determination of the reactive sulfhydryl groups in heme proteins with 4,4′-dipyridinedisulfide

1969 • 62 citations

Current status of artificial oxygen carriers

2000 • 61 citations

Exchange of Subunit Interfaces between Recombinant Adult and Fetal Hemoglobins

1997 • 54 citations

Influence of intramolecular cross-links on the molecular, structural and functional properties of PEGylated haemoglobin

2007 • 51 citations

Kinetic and Equilibrium Properties of Hemoglobin Kansas

1973 • 45 citations

Haemoglobin: The surface buried between the α1β1 and α2β2 dimers in the deoxy and oxy structures

1985 • 43 citations

Extension Arm Facilitated PEGylation of Hemoglobin: Correlation of the Properties with the Extent of PEGylation

2006 • 43 citations

Selective carboxymethylation of the alpha-amino groups of hemoglobin. Effect on functional properties.

1983 • 43 citations

Hemoglobin Rothschild (β37(C3)Trp → Arg): A high/low affinity hemoglobin mutant

1980 • 38 citations

Hemoglobin Thionville. An alpha-chain variant with a substitution of a glutamate for valine at NA-1 and having an acetylated methionine NH2 terminus.

1992 • 31 citations

Subunit dissociation of certain abnormal human hemoglobins

1969 • 31 citations

[13] Stationary and time-resolved circular dichroism of hemoglobins

1994 • 25 citations

The dimer-tetramer equilibrium of recombinant hemoglobins. Stabilization of the α1β2 interface by the mutation β(Cys112 → Gly) at the α1β1 interface

1994 • 23 citations

Allosteric Effectors Influence the Tetramer Stability of Both R- and T-states of Hemoglobin A

2006 • 22 citations

N‐terminal acetylation and protonation of individual hemoglobin subunits: Position‐dependent effects on tetramer strength and cooperativity

2005 • 18 citations

Evidence of hemoglobin dissociation

1994 • 16 citations

Hemoglobin of the Antarctic Fishes Trematomus bernacchii and Trematomus newnesi: Structural Basis for the Increased Stability of the Liganded Tetramer Relative to Human Hemoglobin

2001 • 11 citations

Double mixing stopped-flow method for the study of equilibria and kinetics of dimer-tetramer association of hemoglobins: Studies on Hb Carp, Hb A, and Hb Rothschild

1991 • 10 citations