Mass spectrometry and proteomics

Initial sequencing and analysis of the human genome

2001 • 23,520 citations

Probability-based protein identification by searching sequence databases using mass spectrometry data

1999 • 8,138 citations

An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

1994 • 6,412 citations

Large-scale analysis of the yeast proteome by multidimensional protein identification technology

2001 • 4,766 citations

Incidence of Adverse Drug Reactions in Hospitalized Patients

1998 • 4,749 citations

Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

1999 • 4,699 citations

Proteomics to study genes and genomes

2000 • 2,353 citations

Direct analysis of protein complexes using mass spectrometry

1999 • 2,242 citations

Integrated Genomic and Proteomic Analyses of a Systematically Perturbed Metabolic Network

2001 • 2,058 citations

Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags

1994 • 1,475 citations

Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels

1996 • 1,430 citations

The Yeast Nuclear Pore Complex

2000 • 1,383 citations

Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology

2000 • 1,323 citations

Mass Spectrometry in Proteomics

2001 • 1,236 citations

Proteomic analysis of NMDA receptor–adhesion protein signaling complexes

2000 • 1,201 citations

Role of Accurate Mass Measurement (±10 ppm) in Protein Identification Strategies Employing MS or MS/MS and Database Searching

1999 • 1,093 citations

Accurate quantitation of protein expression and site-specific phosphorylation

1999 • 1,014 citations

Immobilized Gallium(III) Affinity Chromatography of Phosphopeptides

1999 • 834 citations

Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome

2001 • 771 citations

A systematic approach to the analysis of protein phosphorylation

2001 • 720 citations

Glycoprotein Structure Determination by Mass Spectrometry

2001 • 570 citations

The Characteristics of Peptide Collision-Induced Dissociation Using a High-Performance MALDI-TOF/TOF Tandem Mass Spectrometer

1999 • 532 citations

Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex

1998 • 486 citations

Protein microarrays: prospects and problems

2001 • 467 citations

High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

2001 • 465 citations

Analysis of receptor signaling pathways by mass spectrometry: Identification of Vav-2 as a substrate of the epidermal and platelet-derived growth factor receptors

2000 • 409 citations

A Sm-like protein complex that participates in mRNA degradation

2000 • 390 citations

Mass spectrometric approaches for the identification of gel‐separated proteins

1995 • 378 citations

Mass spectrometry

2000 • 355 citations

Pharmacogenomics: Unlocking the Human Genome for Better Drug Therapy

2001 • 332 citations

Idiosyncratic drug reactions: the reactive metabolite syndromes

2000 • 329 citations

Detection of Tyrosine Phosphorylated Peptides by Precursor Ion Scanning Quadrupole TOF Mass Spectrometry in Positive Ion Mode

2001 • 299 citations

Electrochemical processes in electrospray ionization mass spectrometry

2000 • 296 citations

The Profile of Soluble Amyloid β Protein in Cultured Cell Media

1996 • 293 citations

MALDI Quadrupole Time-of-Flight Mass Spectrometry: A Powerful Tool for Proteomic Research

2000 • 291 citations

Quantitation and Facilitated de Novo Sequencing of Proteins by Isotopic N-Terminal Labeling of Peptides with a Fragmentation-Directing Moiety

2000 • 282 citations

Perspectives for mass spectrometry and functional proteomics

2001 • 267 citations

Analysis of Noncovalent Complexes of DNA and RNA by Mass Spectrometry

2001 • 263 citations

Parent Ion Scans of Unseparated Peptide Mixtures

1996 • 250 citations

Mass spectrometry: A tool for the identification of proteins separated by gels

2000 • 248 citations

Mass spectrometry for biotechnology

1997 • 225 citations

High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation

2000 • 219 citations

Isoelectric Focusing Nonporous RP HPLC: A Two-Dimensional Liquid-Phase Separation Method for Mapping of Cellular Proteins with Identification Using MALDI-TOF Mass Spectrometry

2000 • 212 citations

High Throughput Proteome-Wide Precision Measurements of Protein Expression Using Mass Spectrometry

1999 • 211 citations

Mass Spectrometric Resolution of Reversible Protein Phosphorylation in Photosynthetic Membranes ofArabidopsis thaliana

2001 • 211 citations

A Generic Strategy To Analyze the Spatial Organization of Multi-Protein Complexes by Cross-Linking and Mass Spectrometry

1999 • 205 citations

Mass Spectrometry in the Clinical Laboratory

2001 • 202 citations

Strategy for qualitative and quantitative analysis in proteomics based on signature peptides

2000 • 191 citations

Proteomics on a chip: Promising developments

2001 • 189 citations

Quantitative Proteomic Analysis Using a MALDI Quadrupole Time-of-Flight Mass Spectrometer

2001 • 179 citations

Proteomics meets cell biology: The establishment of subcellular proteomes

2000 • 175 citations

Biosensor chip mass spectrometry: A chip-based proteomics approach

2000 • 171 citations

Measuring gene expression by quantitative proteome analysis

2000 • 159 citations

Automated LC–LC–MS–MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses

2001 • 152 citations

Direct genetic analysis by matrix-assisted laser desorption/ionization mass spectrometry

1999 • 142 citations

Protein Identification with a Single Accurate Mass of a Cysteine-Containing Peptide and Constrained Database Searching

2000 • 142 citations

Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry

1998 • 139 citations

Identifying the proteome: software tools

2000 • 134 citations

Bactericidal Domain of Lactoferrin: Detection, Quantitation, and Characterization of Lactoferricin in Serum by SELDI Affinity Mass Spectrometry

1998 • 128 citations

Cancer proteomics: from biomarker discovery to signal pathway profiling.

2001 • 127 citations

Separation and Identification of Peptides from Gel-Isolated Membrane Proteins Using a Microfabricated Device for Combined Capillary Electrophoresis/Nanoelectrospray Mass Spectrometry

2000 • 126 citations

Use of mass spectrometry-derived data to annotate nucleotide and protein sequence databases

2001 • 125 citations

A Molecular Scanner To Automate Proteomic Research and To Display Proteome Images

1999 • 124 citations

Monitoring Enzyme Catalysis with Mass Spectrometry

2000 • 123 citations

Profiling of amyloid beta peptide variants using SELDI Protein Chip arrays.

1999 • 120 citations

Separations Combined with Mass Spectrometry

2001 • 120 citations

Back to the future: The human protein index (HPI) and the agenda for post-proteomic biology

2001 • 119 citations

Rapidly switchable matrix-assisted laser desorption/ionization and electrospray quadrupole-time-of-flight mass spectrometry for protein identification

2000 • 119 citations

Peer Reviewed: Detecting Proteins Separated by 2-D Gel Electrophoresis.

2000 • 118 citations

Toward a Clinical Molecular Scanner for Proteome Research: Parallel Protein Chemical Processing before and during Western Blot

1999 • 117 citations

Applications of isotope dilution‐mass spectrometry in clinical chemistry, pharmacokinetics, and toxicology

1992 • 110 citations

Better approaches to finding the needle in a haystack: Optimizing proteome analysis through automation

2000 • 109 citations

Identification of a Phosphorylation Site in the Hinge Region of the Human Progesterone Receptor and Additional Amino-terminal Phosphorylation Sites

2001 • 106 citations

Microfabricated Polymer Devices for Automated Sample Delivery of Peptides for Analysis by Electrospray Ionization Tandem Mass Spectrometry

1999 • 100 citations

Simplification of complex peptide mixtures for proteomic analysis: Reversible biotinylation of cysteinyl peptides

2000 • 99 citations

Mass Spectrometry in Biology & Medicine

2000 • 90 citations

A Strategy for Rapid, High-Confidence Protein Identification

1997 • 90 citations

BIA/MS of Epitope-Tagged Peptides Directly from E. Coli Lysate: Multiplex Detection and Protein Identification at Low-Femtomole to Subfemtomole Levels

1999 • 84 citations

Matrix-Assisted Laser Desorption/Ionization Coupled with Quadrupole/Orthogonal Acceleration Time-of-Flight Mass Spectrometry for Protein Discovery, Identification, and Structural Analysis

2001 • 80 citations

Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry

2001 • 79 citations

Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry

2003 • 77 citations

Characterization of the Glycosylation Sites in Cyclooxygenase-2 Using Mass Spectrometry

2001 • 76 citations

Sequencing the Entire Genomes of Free-Living Organisms: The Foundation of Pharmacology in the New Millennium

2000 • 75 citations

Advances in surface plasmon resonance biomolecular interaction analysis mass spectrometry (BIA/MS)

1999 • 75 citations

Detection and Quantification of β-2-Microglobulin Using Mass Spectrometric Immunoassay

2001 • 72 citations

Differential Screening and Mass Mapping of Proteins from Premalignant and Cancer Cell Lines Using Nonporous Reversed-Phase HPLC Coupled with Mass Spectrometric Analysis

2001 • 71 citations

Fluorescence detection of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution

2000 • 71 citations

Mass Spectrometry in Viral Proteomics

2000 • 69 citations

Mass spectrometry in the search for uremic toxins

1997 • 58 citations

High sensitivity mass spectrometric methods for obtaining intact molecular weights from gel-separated proteins

1999 • 57 citations

Pharmacogenomics: The Genomics of Drug Response

2000 • 53 citations

Characterization of Enzymatic Processes by Rapid Mix−Quench Mass Spectrometry: The Case of dTDP-glucose 4,6-Dehydratase

2000 • 50 citations

Analysis of Enzyme Kinetics Using Electrospray Ionization Mass Spectrometry and Multiple Reaction Monitoring: Fucosyltransferase V

2001 • 50 citations

Rapid genotyping by MALDI-monitored nuclease selection from probe libraries

2000 • 47 citations

Integrating molecular medicine with functional proteomics: Realities and expectations

2001 • 41 citations

Imaging and detection technologies for image analysis in electrophoresis

2001 • 41 citations

Electrospray ionization and matrix-assisted laser desorption ionization mass spectrometry

2000 • 38 citations

Proteomics — the protein expression technology to study connective tissue biology

2001 • 37 citations

Biological mass spectrometry: a primer

2000 • 35 citations

Peer Reviewed: Integrated Genomic/Proteomic Analysis

1999 • 32 citations

Site-Specific Phosphorylation of Human p53 Protein Determined by Mass Spectrometry

2001 • 30 citations

Affinity Mass Spectrometry-Based Approaches for the Analysis of Protein-Protein Interaction and Complex Mixtures of Peptide-Ligands

1999 • 25 citations

Direct Analysis of the Products of Sequential Cleavages of Peptides and Proteins Affinity-Bound to Immobilized Metal Ion Beads by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

1999 • 21 citations

A comprehensive characterization of the T-cell antigen receptor complex composition by microcapillary liquid chromatography-tandem mass spectrometry

2000 • 18 citations

A comprehensive characterization of the T‐cell antigen receptor complex composition by microcapillary liquid chromatography‐tandem mass spectrometry

2000 • 18 citations

<title>Mass spectrometric immunoassay</title>

1996 • 17 citations

Allele-Specific HLA-DR Typing by Mass Spectrometry: An Alternative to Hybridization-Based Typing Methods

2000 • 12 citations

Using mass spectrometry for quantitative proteomics

2000 • 11 citations

Integrating expression-based drug response and SNP-based pharmacogenetic strategies into a single comprehensive pharmacogenomics program

2000 • 9 citations