Low-Affinity Binding Determined by Titration Calorimetry Using a High-Affinity Coupling Ligand: A Thermodynamic Study of Ligand Binding to Protein Tyrosine Phosphatase 1B

Rapid measurement of binding constants and heats of binding using a new titration calorimeter

1989 • 2,603 citations

Fundamentals of analytical chemistry

1963 • 2,279 citations

Fundamentals of analytical chemistry

2021 • 1,753 citations

On the attribution and additivity of binding energies

1981 • 902 citations

Binding of a high affinity phosphotyrosyl peptide to the Src SH2 domain: Crystal structures of the complexed and peptide-free forms

1993 • 758 citations

Crystal Structure of Human Protein Tyrosine Phosphatase 1B

1994 • 747 citations

Structural Basis for Phosphotyrosine Peptide Recognition by Protein Tyrosine Phosphatase 1B

1995 • 575 citations

Recognition of a high-affinity phosphotyrosyl peptide by the Src homology-2 domain of p56lck

1993 • 497 citations

Evidence for protein-tyrosine-phosphatase catalysis proceeding via a cysteine-phosphate intermediate.

1991 • 468 citations

Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: A paradigm for inhibitor design

1997 • 392 citations

Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 Å and the complex with tungstate

1994 • 392 citations

Sensing the heat: the application of isothermal titration calorimetry to thermodynamic studies of biomolecular interactions

1996 • 341 citations

Protein-Tyrosine Phosphatases: Biological Function, Structural Characteristics, and Mechanism of Catalysis

1998 • 261 citations

Transition metal ion inhibition of enzyme-catalyzed phosphate ester displacement reactions

1974 • 200 citations

Protein Tyrosine Phosphatase Substrate Specificity: Size and Phosphotyrosine Positioning Requirements in Peptide Substrates

1994 • 171 citations

Structural energetics of peptide recognition: Angiotensin II/antibody binding

1993 • 167 citations

Phosphatidylinositol 3-kinase p85 SH2 domain specificity defined by direct phosphopeptide/SH2 domain binding

1993 • 162 citations

The Cys(X)5Arg Catalytic Motif in Phosphoester Hydrolysis

1994 • 159 citations

Dissecting the energetics of a protein-protein interaction: the binding of ovomucoid third domain to elastase 1 1Edited by P. E. Wright

1997 • 157 citations

Purification and characterization of a low-molecular-weight acid phosphatase—A phosphotyrosyl-protein phosphatase from bovine heart

1990 • 128 citations

A Continuous Spectrophotometric and Fluorometric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides

1993 • 117 citations

Why Is Phosphonodifluoromethyl Phenylalanine a More Potent Inhibitory Moiety Than Phosphonomethyl Phenylalanine Toward Protein-Tyrosine Phosphatases

1995 • 106 citations

Nature of the rate-determining steps of the reaction catalyzed by the Yersinia protein-tyrosine phosphatase.

1994 • 94 citations

Kinetic and Mechanistic Characterization of a Mammalian Protein-tyrosine Phosphatase, PTP1

1995 • 89 citations

Stromelysin Inhibitors Designed from Weakly Bound Fragments: Effects of Linking and Cooperativity

1997 • 85 citations

Thermodynamics of Carbohydrate Binding to Galectin-1 from Chinese Hamster Ovary Cells and Two Mutants. A Comparison with Four Galactose-Specific Plant Lectins

1996 • 68 citations

Conformationally Constrained Phosphotyrosyl Mimetics Designed as Monomeric src Homology 2 Domain Inhibitors

1995 • 68 citations

Thermodynamics of Inhibitor Binding to the Catalytic Site of Glucoamylase from Aspergillus niger Determined by Displacement Titration Calorimetry

1994 • 57 citations

Specificity of T‐cell protein tyrosine phosphatase toward phosphorylated synthetic peptides

1993 • 55 citations

Thermodynamic Studies of Tyrosyl-Phosphopeptide Binding to the SH2 Domain of p56lck

1994 • 53 citations

Counting the calories to stay in the groove

1995 • 44 citations

Thermodynamics of binding of the carbon dioxide-competitive inhibitor imidazole and related compounds to human carbonic anhydrase I: an isothermal titration calorimetry approach to studying weak binding by displacement with strong inhibitors

1993 • 23 citations

Isothermal Titration Microcalorimetric Studies for the Binding of Octenoyl-CoA to Medium Chain Acyl-CoA Dehydrogenase

1997 • 19 citations