The Polymerase Chain Reaction

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Avoiding false positives with PCR

1989 • 3,564 citations

A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1988 • 2,410 citations

Mitochondrial DNA Mutation Associated with Leber's Hereditary Optic Neuropathy

1988 • 2,368 citations

Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes

1988 • 2,121 citations

HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitus

1987 • 2,045 citations

Genetic applications of an inverse polymerase chain reaction.

1988 • 1,867 citations

Prevalence of ras gene mutations in human colorectal cancers

1987 • 1,866 citations

Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes

1986 • 1,863 citations

Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

1988 • 1,582 citations

Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification

1988 • 1,260 citations

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

1989 • 991 citations

DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear Cells

1988 • 979 citations

Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction

1989 • 895 citations

A procedure forin vitroamplification of DNA segments that lie outside the boundaries of known sequences

1988 • 814 citations

An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA Sequences

1987 • 811 citations

DNA typing from single hairs

1988 • 809 citations

Targetted correction of a mutant HPRT gene in mouse embryonic stem cells

1987 • 786 citations

Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences

1986 • 766 citations

Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.

1988 • 748 citations

Amplification and analysis of DNA sequences in single human sperm and diploid cells

1988 • 685 citations

Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA

1987 • 514 citations

Mitochondrial DNA sequences from a 7000-year old brain

1988 • 492 citations

Detection of Cytomegalovirus in Urine from Newborns by Using Polymerase Chain Reaction DNA Amplification

1988 • 490 citations

BIOPSY OF HUMAN PREIMPLANTATION EMBRYOS AND SEXING BY DNA AMPLIFICATION

1989 • 489 citations

CONTROLLED TRIAL OF SOYA-BEAN OIL IN MYOCARDIAL INFARCTION

1968 • 473 citations

Human Immunodeficiency Virus Type 1 Infection in Homosexual Men Who Remain Seronegative for Prolonged Periods

1989 • 460 citations

High Rate of HTLV-II Infection in Seropositive IV Drug Abusers in New Orleans

1989 • 426 citations

Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction

1989 • 407 citations

Allelic sequence variation of the HLA-DQ loci: relationship to serology and to insulin-dependent diabetes susceptibility.

1988 • 378 citations

AVIRULENT CORYNEBACTERIA

1975 • 371 citations

Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes.

1988 • 367 citations

Use of the Polymerase Chain Reaction for Early Detection of the Proviral Sequences of Human Immunodeficiency Virus in Infants Born to Seropositive Mothers

1989 • 352 citations

Thermostable DNA polymerase chain amplification of t(14;18) chromosome breakpoints and detection of minimal residual disease.

1988 • 341 citations

Genomic Amplification with Transcript Sequencing

1988 • 324 citations

SIMPLE NON-INVASIVE METHOD TO OBTAIN DNA FOR GENE ANALYSIS

1988 • 321 citations

Detection of BK virus and JC virus in urine and brain tissue by the polymerase chain reaction

1989 • 314 citations

Diagnosis of Sickle Cell Anemia and β-Thalassemia with Enzymatically Amplified DNA and Nonradioactive Allele-Specific Oligonucleotide Probes

1988 • 297 citations

Detection of Cytomegalovirus DNA in Peripheral Blood of Patients Infected with Human Immunodeficiency Virus

1988 • 296 citations

DNA Diagnostics—Molecular Techniques and Automation

1988 • 286 citations

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA.

1989 • 286 citations

Detection of Human T-Cell Lymphoma/Leukemia Virus Type I DNA and Antigen in Spinal Fluid and Blood of Patients with Chronic Progressive Myelopathy

1988 • 279 citations

The polymerase chain reaction: a new epidemiological tool for investigating cervical human papillomavirus infection.

1989 • 277 citations

Specific DNA amplification

1988 • 262 citations

Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides

1987 • 260 citations

Enzymatic Gene Amplification: Qualitative and Quantitative Methods for Detecting Proviral DNA Amplified in Vitro

1988 • 242 citations

A Newly Characterized HLA DQ _β Allele Associated with Pemphigus Vulgaris

1988 • 230 citations

Construction of mutant and chimeric genes using the polymerase chain reaction

1989 • 224 citations

HLA class II allelic variation and susceptibility to pemphigus vulgaris.

1988 • 211 citations

Detection of Human Papillomavirus in Formalin-Fixed, Invasive Squamous Carcinomas Using the Polymerase Chain Reaction

1989 • 209 citations

Polymerase Chain Reaction

1988 • 207 citations

Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase

1989 • 206 citations

The Polymerase Chain Reaction

1995 • 200 citations

IDENTIFICATION OF HIV-INFECTED SERONEGATIVE INDIVIDUALS BY A DIRECT DIAGNOSTIC TEST BASED ON HYBRIDISATION TO AMPLIFIED VIRAL DNA

1988 • 195 citations

Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular biology and classical histology.

1988 • 190 citations

Whole genome PCR: application to the identification of sequences bound by gene regulatory protein

1989 • 187 citations

DNA of Human Papillomavirus Type 16 in Dysplastic and Malignant Lesions of the Conjunctiva and Cornea

1989 • 174 citations

Mixed Human Immunodeficiency Virus (HIV) Infection in an Individual: Demonstration of Both HIV Type 1 and Type 2 Proviral Sequences by Using Polymerase Chain Reaction

1988 • 160 citations

T-cell receptor V alpha and C alpha alleles associated with multiple and myasthenia gravis.

1989 • 158 citations

A Mitochondrial DNA Mutation as a Cause of Leber's Hereditary Optic Neuropathy

1989 • 158 citations

HTLV-I-induced lymphoma mimicking Hodgkin's disease. Diagnosis by polymerase chain reaction amplification of specific HTLV-I sequences in tumor DNA

1988 • 154 citations

Species-specific detection of Legionella pneumophila in water by DNA amplification and hybridization

1989 • 152 citations

POLYMERASE CHAIN REACTION FOR DETECTION OF RESIDUAL LEUKAEMIA

1989 • 152 citations

DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

1988 • 149 citations

Colorimetric detection of specific DNA segments amplified by polymerase chain reactions.

1989 • 143 citations

ECZEMA HERPETICUM

1959 • 142 citations

Direct sequencing of enzymatically amplified human genomic DNA.

1988 • 141 citations

SCREENING FOR PHENYLKETONURIA MUTATIONS BY DNA AMPLIFICATION WITH THE POLYMERASE CHAIN REACTION

1988 • 137 citations

Rapid Prenatal Diagnosis of Sickle Cell Anemia by a New Method of DNA Analysis

1987 • 132 citations

Enzymatic Amplification of Hepatitis B Virus DNA in Serum Compared with Infectivity Testing in Chimpanzees

1989 • 121 citations

Rapid Detection of Cutaneous Herpes Simplex Virus Infection with the Polymerase Chain Reaction

1989 • 119 citations

Rapid detection of human parvovirus B19 DNA by dot-hybridization and the polymerase chain reaction

1989 • 112 citations

Polymerase chain reaction: replication errors and reliability of gene diagnosis

1989 • 112 citations

Primer-mediated enzymatic amplification of cytomegalovirus (CMV) DNA. Application to the early diagnosis of CMV infection in marrow transplant recipients.

1989 • 105 citations

Truncated variants of apolipoprotein B cause hypobetalipoproteinaemia

1988 • 102 citations

Diagnosis of Genetic Disorders at the DNA Level

1989 • 102 citations

A sensitive method for the identification of uncharacterized viruses related to known virus groups: hepadnavirus model system.

1988 • 95 citations

Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction

1989 • 94 citations

Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection

1989 • 81 citations

Polymerase chain reaction for fast, nonradioactive detection of high‐ and low‐risk papillomavirus types in routine cervical specimens and in biopsies

1989 • 80 citations

Direct characterization of factor VIII in plasma: detection of a mutation altering a thrombin cleavage site (arginine-372----histidine).

1989 • 79 citations

DETECTION OF POLYMORPHISMS AT CYTOSINE PHOSPHOGUANADINE DINUCLEOTIDES AND DIAGNOSIS OF HAEMOPHILIA B CARRIERS

1989 • 75 citations

Enzymatic amplification of exogenous and endogenous retroviral sequences from DNA of patients with tropical spastic paraparesis.

1988 • 65 citations

POLYMERASE CHAIN REACTION FOR DETECTION OF DISSEMINATION IN GASTRIC LYMPHOMA

1989 • 45 citations

HIV viral sequences in seronegative people at risk detected by in situ hybridisation and polymerase chain reaction.

1989 • 42 citations

Rapid prenatal diagnosis of beta thalassemia using DNA amplification and nonradioactive probes

1989 • 41 citations

MOLECULAR SCREENING FOR HAEMOGLOBIN CONSTANT SPRING

1989 • 37 citations

Hemophilia B Durham: a mutation in the first EGF-like domain of factor IX that is characterized by polymerase chain reaction

1988 • 37 citations

Rapid prenatal diagnosis of beta thalassemia using DNA amplification and nonradioactive probes

1989 • 31 citations

Prenatal exclusion testing for Huntington disease using the polymerase chain reaction

1989 • 26 citations

Hemophilia B Durham: a mutation in the first EGF-like domain of factor IX that is characterized by polymerase chain reaction

1988 • 23 citations

Characterization of single base substitutions In edited apolipoprotein B transcripts

1989 • 17 citations

Viral Diagnosis Using DNA‐Based Probes

1988 • 12 citations

Use of In Vitro DNA Amplification to Screen Family Members for an Insulin Gene Mutation

1988 • 11 citations

Use of in vitro DNA amplification to screen family members for an insulin gene mutation

1988 • 8 citations

Deleted Work

1955 • 0 citations