Site-directed mutagenesis of putative active-site amino acid residues of 3,2-trans-enoyl-CoA isomerase, conserved within the low-homology isomerase/hydratase enzyme family

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Basic local alignment search tool

1990 • 87,358 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250

1977 • 2,970 citations

A Protein Sequenator

1967 • 2,826 citations

The Specificity of Triglyceride Synthesis from Diglycerides in Chicken Adipose Tissue

1961 • 402 citations

Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases

1992 • 194 citations

Peroxisomal bifunctional protein from rat liver is a trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities.

1990 • 180 citations

Molecular cloning and nucleotide sequence of the cDNA for rat peroxisomal enoyl-CoA: hydratase-3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme.

1985 • 167 citations

Enzymic isomerization of Δ5-3-ketosteroids

1955 • 139 citations

Degradation of Unsaturated Fatty Acids. Identification of Intermediates in the Degradation of cis-4-Decenoyl-CoA by Extracts of Beef-Liver Mitochondria

1978 • 135 citations

Kinetic and ultraviolet spectroscopic studies of active-site mutants of .DELTA.5-3-ketosteroid isomerase

1989 • 122 citations

Superoxide dismutase: “Positive” spectrophotometric assays

1977 • 110 citations

A re-examination of some properties of fatty acyl-CoA micelles.

1981 • 93 citations

Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes

1990 • 91 citations

[Metabolism of unsaturated fatty acids. V. On the beta-oxidation of mono- and polyene-fatty acids. Mechanism of enzymatic reactions of delta-2-cis-enoyl-CoA compounds].

1965 • 91 citations

Molecular cloning and sequence analysis of the cDNA for rat mitochondrial enoyl‐CoA hydratase

1989 • 76 citations

Crotonase-catalyzed .beta.-elimination is concerted: a double isotope effect study

1991 • 72 citations

Amino acid sequence similarities of the mitochondrial short chain delta 3, delta 2-enoyl-CoA isomerase and peroxisomal multifunctional delta 3, delta 2-enoyl-CoA isomerase, 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase enzyme in rat liver. The proposed occurrence of isomerization and hydration in the same catalytic domain of the multifunctional enzyme

1991 • 63 citations

Evidence that the fadB gene of the fadAB operon of Escherichia coli encodes 3-hydroxyacyl-coenzyme A (CoA) epimerase, delta 3-cis-delta 2-trans-enoyl-CoA isomerase, and enoyl-CoA hydratase in addition to 3-hydroxyacyl-CoA dehydrogenase

1988 • 53 citations

[93] Crystalline crotonase1 from ox liver

1955 • 49 citations

Purification of the Multienzyme Complex for Fatty Acid Oxidation from Pseudomonas fragi and Reconstitution of the Fatty Acid Oxidastion System1

1990 • 47 citations

Sequence organization and regulation of the Bacillus subtilis menBE operon

1992 • 37 citations

Mitochondrial 3-2trans-Enoyl-CoA Isomerase. Purification, Cloning, Expression, and Mitochondrial Import of the Key Enzyme of Unsaturated Fatty acidβ-Oxidation

1991 • 35 citations

[17] Δ3-cis,Δ2-trans-Enoyl-CoA isomerase from rat liver mitochondria

1969 • 32 citations

Purification and Properties of 3-cis-2-trans-Enoyl-CoA Isomerase (Dodecenoyl-CoA Δ-Isomerase) from Rat Liver Mitochondria

1978 • 29 citations

Der Stoffwechsel der ungesättigten Fettsäuren, IV. Zur β-Oxydation der Mono- und Polyenfettsäuren Chemische Synthesen von Intermediärprodukten

1964 • 28 citations

Primary Structures of the Genes, faoA and faoB, from Pseudomonas fragi B-0771 Which Encode the Two Subunits of the HDT Multienzyme Complex Involved in Fatty Acid β-Oxidation1

1992 • 27 citations

Purification and Characterization of Bovine Liver.3-cis-2-trans-Enoyl-CoAIsomerase

1990 • 14 citations