High Level Escherichia coli Expression and Production of a Bivalent Humanized Antibody Fragment

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Tissue sulfhydryl groups

1959 • 25,130 citations

Human Breast Cancer: Correlation of Relapse and Survival with Amplification of the HER-2/ neu Oncogene

1987 • 11,429 citations

Studies of the HER-2/ neu Proto-Oncogene in Human Breast and Ovarian Cancer

1989 • 6,734 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Sequences of proteins of immunological interest

1984 • 3,427 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Humanization of an anti-p185HER2 antibody for human cancer therapy.

1992 • 1,867 citations

Reshaping human antibodies for therapy

1988 • 1,555 citations

A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study

1974 • 1,384 citations

Canonical structures for the hypervariable regions of immunoglobulins

1987 • 1,357 citations

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1986 • 1,356 citations

Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9

1977 • 1,055 citations

Assembly of a Functional Immunoglobulin F _v Fragment in Escherichia coli

1988 • 973 citations

REMISSION INDUCTION IN NON-HODGKIN LYMPHOMA WITH RESHAPED HUMAN MONOCLONAL ANTIBODY CAMPATH-1H

1988 • 703 citations

p185^HER2 Monoclonal Antibody Has Antiproliferative Effects In Vitro and Sensitizes Human Breast Tumor Cells to Tumor Necrosis Factor

1989 • 672 citations

Escherichia coli Secretion of an Active Chimeric Antibody Fragment

1988 • 575 citations

Mouse/human chimeric monoclonal antibody in man: kinetics and immune response.

1989 • 526 citations

p185HER2 monoclonal antibody has antiproliferative effects in vitro and sensitizes human breast tumor cells to tumor necrosis factor.

1989 • 479 citations

Characterization of murine monoclonal antibodies reactive to either the human epidermal growth factor receptor or HER2/neu gene product.

1990 • 401 citations

The influence of polyvalency on the binding properties of antibodies

1972 • 396 citations

Reshaping Human Antibodies: Grafting an Antilysozyme Activity

1988 • 348 citations

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1985 • 270 citations

Preparation and performance of bispecific F(ab' gamma)2 antibody containing thioether-linked Fab' gamma fragments.

1987 • 267 citations

Preparation of Bispecific Antibodies by Chemical Recombination of Monoclonal Immunoglobulin G ₁ Fragments

1985 • 249 citations

Gene regulation at the right operator (OR) of bacteriophage λ

1980 • 243 citations

Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12

1986 • 240 citations

Engineering Enzyme Specificity by "Substrate-Assisted Catalysis"

1987 • 219 citations

Immunospecific targeting of liposomes to cells: a novel and efficient method for covalent attachment of Fab' fragments via disulfide bonds

1981 • 214 citations

The nucleotide sequence of a human immnnoglobulin C_γlgene

1982 • 158 citations

Nucleotide sequence of the gene for heat-stable enterotoxin II of Escherichia coli

1983 • 141 citations

Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli

1986 • 140 citations

Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems

1991 • 137 citations

Nucleotide Sequence of a Human Immunoglobulin Cγ4 Gene

1981 • 91 citations

Bispecific F(ab' gamma)2 antibody for the delivery of saporin in the treatment of lymphoma.

1988 • 68 citations

Circular dichroism studies on the acid denaturation of γ-immunoglobulin G and its fragments

1970 • 67 citations

Analysis of protein digests by capillary high-performance liquid chromatography and on-line fast atom bombardment mass spectrometry

1990 • 65 citations

[62] Preparation of F(ab′)2 fragments from mouse IgG of various subclasses

1986 • 50 citations

A cloning cartridge of λ t₀terminator

1987 • 34 citations

Die Primärstruktur einer kristallinen monoklonalen Immunglobulin-L-Kette vom κ-Typ, Subgruppe I (Bence-Jones-Protein Rei.), Isolierung und Charakterisierung der tryptischen Peptide; die vollständige Aminosäuresequenz des Proteins. Ein Beitrag zur Aufklärung der räumlichen Struktur der Antikörper, insbesondere der Haftstelle

1975 • 22 citations

Current perspectives and challenges in the use of monoclonal antibodies as imaging and therapeutic agents

1990 • 22 citations

Recovery of Recombinant Proteins by Immunoaffinity Chromatography

1990 • 15 citations

Mutagenesis facilitated by the removal or introduction of unique restriction sites

1991 • 2 citations