Optimization of retroviral-mediated gene transfer to human NOD/SCID mouse repopulating cord blood cells through a systematic analysis of protocol variables

Production of high-titer helper-free retroviruses by transient transfection.

1993 • 2,280 citations

Improved retroviral vectors for gene transfer and expression.

1989 • 1,997 citations

Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice

1997 • 879 citations

Versatile retroviral vectors for potential use in gene therapy.

1994 • 690 citations

Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/W mice

1985 • 663 citations

Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus

1991 • 647 citations

A newly discovered class of human hematopoietic cells with SCID-repopulating activity

1998 • 640 citations

Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo.

1995 • 606 citations

Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors

1985 • 598 citations

Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.

1994 • 586 citations

A bioactive designer cytokine for human hematopoietic progenitor cell expansion

1997 • 531 citations

Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic– scid/scid mice

1997 • 495 citations

Primitive Human Hematopoietic Cells Are Enriched in Cord Blood Compared With Adult Bone Marrow or Mobilized Peripheral Blood as Measured by the Quantitative In Vivo SCID-Repopulating Cell Assay

1997 • 488 citations

STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.

1994 • 459 citations

Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium.

1996 • 393 citations

Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.

1996 • 360 citations

Human bone marrow CD34- cells engraft in vivo and undergo multilineage expression that includes giving rise to CD34+ cells.

1998 • 346 citations

Cytokine manipulation of primitive human hematopoietic cell self-renewal

1997 • 306 citations

Improved Gene Transfer Into Baboon Marrow Repopulating Cells Using Recombinant Human Fibronectin Fragment CH-296 in Combination With Interleukin-6, Stem Cell Factor, FLT-3 Ligand, and Megakaryocyte Growth and Development Factor

1998 • 262 citations

Expression of FLT3 receptor and response to FLT3 ligand by leukemic cells.

1996 • 259 citations

Human FLT3/FLK2 receptor tyrosine kinase is expressed at the surface of normal and malignant hematopoietic cells.

1996 • 250 citations

Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro

1996 • 240 citations

Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor

1996 • 234 citations

Optimization of Fibronectin-Assisted Retroviral Gene Transfer into Human CD34⁺Hematopoietic Cells

1997 • 215 citations

Overexpression of HOXB3 in Hematopoietic Cells Causes Defective Lymphoid Development and Progressive Myeloproliferation

1997 • 187 citations

Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice.

1996 • 186 citations

Efficient Retroviral-Mediated Gene Transfer to Human Cord Blood Stem Cells With In Vivo Repopulating Potential

1998 • 161 citations

Stem cell factor, interleukin-3, and interleukin-6 promote retroviral- mediated gene transfer into murine hematopoietic stem cells

1992 • 147 citations

Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures

1995 • 131 citations

Differential growth factor requirement of primitive cord blood hematopoietic stem cell for self-renewal and amplification vs proliferation and differentiation

1998 • 130 citations

Ontogeny‐associated changes in the cytokine responses of primitive human haemopoietic cells

1998 • 105 citations

Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability.

1989 • 98 citations

Retrovirus-Mediated Gene Transfer into Human CD34⁺38^lowPrimitive Cells Capable of Reconstituting Long-Term CulturesIn Vitroand Nonobese Diabetic–Severe Combined Immunodeficiency MiceIn Vivo

1998 • 83 citations

Rapid and efficient selection of human hematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer

1996 • 78 citations

Report to the NIH Recombinant DNA Advisory Committee on Murine Replication-Competent Retrovirus (RCR) Assays (February 17, 1993)

1993 • 70 citations

High Efficiency Gene Transfer to Human Hematopoietic SCID-Repopulating Cells Under Serum-Free Conditions

1998 • 69 citations

Improved Gene Transfer Into Baboon Marrow Repopulating Cells Using Recombinant Human Fibronectin Fragment CH-296 in Combination With Interleukin-6, Stem Cell Factor, FLT-3 Ligand, and Megakaryocyte Growth and Development Factor

1998 • 61 citations

Expression of CD34 on human B cell precursors

1991 • 49 citations

High-Level Gene Transfer to Cord Blood Progenitors Using Gibbon Ape Leukemia Virus Pseudotype Retroviral Vectors and an Improved Clinically Applicable Protocol

1998 • 49 citations

Sustained retroviral gene marking and expression in lymphoid and myeloid cells derived from transduced hematopoietic progenitor cells.

1996 • 29 citations