Generation of a single-chain Fv fragment for the monitoring of deoxycholic acid residues anchored on endogenous proteins

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

1988 • 4,669 citations

Sequences of proteins of immunological interest

1984 • 3,427 citations

Methods and applications

1953 • 1,648 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

Escherichia coli Secretion of an Active Chimeric Antibody Fragment

1988 • 575 citations

Protein modelling for all

1999 • 479 citations

The Bile Acids: Chemistry, Physiology, and Metabolism

1988 • 299 citations

Acyl Glucuronide Drug Metabolites: Toxicological and Analytical Implications

2003 • 255 citations

High level production of soluble single chain antibodies in small-scale Escherichia coli cultures

1997 • 198 citations

An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments.

1994 • 157 citations

Early induction of rat colonic epithelial ornithine and S-adenosyl-L-methionine decarboxylase activities by N-methyl-N'-nitro-N-nitrosoguanidine or bile salts.

1981 • 128 citations

Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain

2004 • 116 citations

Hepatic Disposition of Electrophilic Acyl Glucuronide Conjugates

2000 • 95 citations

Green fluorescent antibodies: novel in vitro tools

2000 • 91 citations

The use of the RACE method to clone hybridoma cDNA when V region primers fail

1994 • 59 citations

A simple method for the determination of dissociation constants by displacement ELISA

2002 • 53 citations

Nature of tissue-bound lithocholic acid and its implications in the role of bile acids in carcinogenesis.

1981 • 48 citations

Separation and Detection of Bile Acid 24-Glucuronides in Human Urine by Liquid Chromatography Combined with Electrospray Ionization Mass Spectrometry

1999 • 47 citations

Construction, Bacterial Expression, and Characterization of Hapten-Specific Single-Chain Fv and Alkaline Phosphatase Fusion Protein

1997 • 42 citations

Effect of Deoxycholic Acid on the Tumour Incidence, Distribution, and Receptor Status of Colorectal Cancer in the Rat Model

1985 • 35 citations

Introduction to molecular immunology

1982 • 34 citations

Separation and Characterization of Carboxyl-linked Glucuronides of Bile Acids in Incubation Mixture of Rat Liver Microsomes

1998 • 33 citations

Introduction to molecular immunology

1983 • 32 citations

Selective PCR Amplification of Functional Immunoglobulin Light Chain from Hybridoma Containing the Aberrant MOPC 21-Derived Vκ by PNA-Mediated PCR Clamping

1999 • 32 citations

Biosynthesis and chemical synthesis of carboxyl-linked glucuronide of lithocholic acid

1992 • 31 citations

Lithocholic acid in human liver: Identification of ∈‐lithocholyl lysine in tissue protein

1977 • 30 citations

Characterization of Cholyl-Adenylate in Rat Liver Microsomes by Liquid Chromatography/Electrospray Ionization–Mass Spectrometry

1999 • 30 citations

Bile acids in tissues: Binding of lithocholic acid to protein

1978 • 29 citations

Stimulation of ornithine decarboxylase activity and DNA synthesis by phorbol esters or bile acids in rat colon.

1984 • 24 citations

Single-chain Fv fragments derived from an anti-11-deoxycortisol antibody

2002 • 24 citations

A monoclonal antibody-based enzyme-linked immunosorbent assay of ursodeoxycholic acid 3-sulfates in human urine

2000 • 23 citations

Bile acid acyl adenylate: a possible intermediate to produce a protein-bound bile acid

2001 • 22 citations

Tissue bile acids in patients with colon cancer and colonic polyps.

1982 • 22 citations

A general method allowing the design of oligonucleotide primers to amplify the variable regions from immunoglobulin cDNA

2003 • 22 citations

A Nonenzymatic Modification of the Amino-terminal Domain of Histone H3 by Bile Acid Acyl Adenylate

2004 • 20 citations

Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR

1995 • 17 citations

Isolation of tissue-bound lithocholic acid from livers of rats treated with methylazoxymethanol

1981 • 16 citations

Production of a Monoclonal Antibody for Sensitive Monitoring of Deoxycholic Acid Residues Anchored on Endogenous Proteins

2000 • 11 citations

Tissue-Bound Bile Acids

1988 • 10 citations

Effects of oral administration of sulfolithocholic acid disodium salt and lithocholic acid sodium salt on N-methyl-N-nitrosourea-induced colonic tumorigenesis in conventional rats.

1989 • 10 citations

Analysis of the Antigen Binding Site of Anti-Deoxycholate Monoclonal Antibody Using a Novel Affinity Labeling Reagent, Acyl Adenylate

2004 • 8 citations