Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

Preparation of transforming deoxyribonucleic acid by phenol treatment

1963 • 1,813 citations

Preparative and analytical purification of DNA from agarose.

1979 • 1,585 citations

The Release of Enzymes from Escherichia coli by Osmotic Shock and during the Formation of Spheroplasts

1965 • 1,490 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

High recovery of tryptophan from acid hydrolysates of proteins

1969 • 678 citations

An Extracellular Poly(3‐Hydroxybutyrate) Depolymerase from Alcaligenes faecalis

1982 • 324 citations

Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

1983 • 307 citations

A Study of the Substrate Specificity and Other Properties of the Alkaline Phosphatase of Escherichia coli

1962 • 272 citations

Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites

1978 • 246 citations

Decomposition of Poly-β-Hydroxybutyrate by Pseudomonads

1965 • 246 citations

Biotechnology. An industrial view

1982 • 220 citations

[79] Alcohol dehydrogenase from baker's yeast

1955 • 198 citations

SOME ASPECTS OF THE ENDOGENOUS METABOLISM OF BACTERIA

1964 • 189 citations

Degradation of poly(3-hydroxybutyrate) by poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1

1986 • 188 citations

Poly-?-hydroxybutters�ure abbauende Bakterien und Exoenzym

1963 • 145 citations

Effect of limited tryptic modification of a bacterial poly(3-hydroxybutyrate) depolymerase on its catalytic activity

1988 • 96 citations

Poly-beta-hydroxybutyrate depolymerases of Pseudomonas lemoignei.

1966 • 95 citations

A new isolation method of plasmid deoxyribonucleic acid from Staphylococcus aureus using a lytic enzyme of Achromobacter lyticus.

1977 • 16 citations