Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1988 • 662 citations

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

1988 • 539 citations

Cloned human phenylalanine hydroxylase gene allows prenatal diagnosis and carrier detection of classical phenylketonuria

1983 • 334 citations

Nucleotide sequence of a full-length complementary DNA clone and amino acid sequence of human phenylalanine hydroxylase

1985 • 325 citations

Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12.

1984 • 279 citations

Molecular Basis of Phenotypic Heterogeneity in Phenylketonuria

1991 • 239 citations

A simple purification of phenylalanine hydroxylase by substrate-induced hydrophobic chromatography.

1979 • 191 citations

In vitro activation of rat liver phenylalanine hydroxylase by phosphorylation.

1976 • 149 citations

Gene Transfer and Expression of Human Phenylalanine Hydroxylase

1985 • 131 citations

The Phenylalanine Hydroxylating System

1993 • 125 citations

Homology between phenylalanine and tyrosine hydroxylases reveals common structural and functional domains

1985 • 121 citations

Molecular basis of phenylketonuria and related hyperphenylalaninemias: Mutations and polymorphisms in the human phenylalanine hydroxylase gene

1992 • 94 citations

Sequencing of peptides and proteins from the carboxy terminus

1992 • 85 citations

The effect of ligands of phenylalanine 4-monooxygenase on the cAMP-dependent phosphorylation of the enzyme.

1984 • 75 citations

The isolation and properties of phenylalanine hydroxylase from rat liver

1974 • 69 citations

Relationship between the substrate activation site and catalytic site of phenylalanine hydroxylase.

1980 • 61 citations

Amino acid sequence at the phosphorylated site of rat liver phenylalanine hydroxylase and phosphorylation of a corresponding synthetic peptide

1980 • 60 citations

Biochemical characterization of recombinant human phenylalanine hydroxylase produced in Escherichia coli.

1987 • 56 citations

A monoclonal antibody to aromatic amino acid hydroxylases. Identification of the epitope

1988 • 48 citations

Two apparent molecular weight forms of human and monkey phenylalanine hydroxylase are due to phosphorylation.

1984 • 42 citations

Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria

1979 • 42 citations

Phenylalanine 4-monooxygenase from bovine and rat liver: Some physical and chemical properties

1982 • 36 citations

Some characteristics of partially purified human liver phenylalanine hydroxylase

1973 • 28 citations

BPTI and N-terminal extended analogues generated by factor Xa cleavage and cathepsin C trimming of a fusion protein expressed in Escherichia coli

1991 • 28 citations

Expression of Wild Type and Mutant Forms of Human Phenylalanine Hydroxylase in E. Coli

1993 • 23 citations

The isolation, properties, and assay of phenylalanine hydroxylase from human and rat liver

1976 • 8 citations

Purification of phenylalanine hydroxylase from human adult and foetal livers with a monoclonal antibody

1985 • 8 citations

The immunological evidence for a phenylalanine hydroxylase like immunoreactive protein in different human cells and tissues

1990 • 6 citations

Phenylalanine hydroxylase. Evidence that the enzyme from human liver might not be a phosphoprotein.

1983 • 4 citations

Membrane-bound phenylalanine hydroxylase of human liver

1992 • 1 citations