Distinct biochemical properties of the native members of the G12 G-protein subfamily. Characterization of Gα12 purified from rat brain

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

A simplification of the protein assay method of Lowry et al. which is more generally applicable

1977 • 8,485 citations

Diversity of G Proteins in Signal Transduction

1991 • 1,990 citations

Heterotrimeric C proteins: Organizers of transmembrane signals

1995 • 1,388 citations

The 2.0 Å crystal structure of a heterotrimeric G protein

1996 • 1,118 citations

The structure of the G protein heterotrimer Giα1β1γ2

1995 • 1,091 citations

Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis

1994 • 823 citations

The 2.2 Å crystal structure of transducin-α complexed with GTPγS

1993 • 788 citations

Gα12 and Gα13 Stimulate Rho-dependent Stress Fiber Formation and Focal Adhesion Assembly

1995 • 443 citations

G proteins of the G12 family are activated via thromboxane A2 and thrombin receptors in human platelets.

1994 • 441 citations

Alternative splicing produces a divergent cytoplasmic tail in the human endothelial thromboxane A2 receptor.

1994 • 357 citations

Purification of Recombinant G Proteins from Sf9 Cells by Hexahistidine Tagging of Associated Subunits

1995 • 332 citations

Tertiary and Quaternary Structural Changes in G _iα1 Induced by GTP Hydrolysis

1995 • 294 citations

Purification from Sf9 cells and characterization of recombinant Gq alpha and G11 alpha. Activation of purified phospholipase C isozymes by G alpha subunits

1993 • 262 citations

G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits.

1991 • 250 citations

Activation of Jun Kinase/Stress-activated Protein Kinase by GTPase-deficient Mutants of Gα12 and Gα13

1995 • 178 citations

A mutant alpha subunit of G12 potentiates the eicosanoid pathway and is highly oncogenic in NIH 3T3 cells.

1993 • 176 citations

G alpha 13 stimulates Na-H exchange.

1994 • 175 citations

Characterization of transducin from bovine retinal rod outer segments. Participation of the amino-terminal region of T alpha in subunit interaction.

1987 • 131 citations

Receptors and G proteins as primary components of transmembrane signal transduction

1995 • 123 citations

Protein kinase C-dependent and -independent activation of Na+/H+ exchanger by G alpha 12 class of G proteins.

1994 • 109 citations

Mannose 6-Phosphate/Insulin-like Growth Factor II Receptor Fails to Interact with G-proteins

1995 • 106 citations

The amino terminus of G protein alpha subunits is required for interaction with beta gamma.

1988 • 104 citations

The transforming activity of activated Gα12

1993 • 100 citations

Characterization of [5,6-3H]SQ 29,548 as a high affinity radioligand, binding to thromboxane A2/prostaglandin H2-receptors in human platelets.

1988 • 87 citations

The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G‐protein‐coupled receptors

1995 • 77 citations

Purification and characterization of the alpha subunit of G13.

1994 • 75 citations

Cyclic GMP-dependent Protein Kinase Blocks Pertussis Toxin-sensitive Hormone Receptor Signaling Pathways in Chinese Hamster Ovary Cells

1995 • 67 citations

Isolation of the alpha subunits of GTP-binding regulatory proteins by affinity chromatography with immobilized beta gamma subunits.

1989 • 65 citations

G12 and G13 α-Subunits Are Immunochemically Detectable in Most Membranes of Various Mammalian Cells and Tissues

1994 • 64 citations

The α‐subunits of G‐proteins G₁₂ and G₁₃ are palmitoylated, but not amidically myristoylated

1994 • 63 citations

The disaggregation theory of signal transduction revisited: further evidence that G proteins are multimeric and disaggregate to monomers when activated.

1993 • 59 citations

Purification of a novel G-protein α0-subtype from mammalian brain

1994 • 50 citations

Deletion within the amino-terminal region of Gs alpha impairs its ability to interact with beta gamma subunits and to activate adenylate cyclase

1991 • 50 citations

Receptor-effector coupling, A practical approach

1991 • 46 citations

Octyl glucoside extracts GTP-binding regulatory proteins from rat brain "synaptoneurosomes" as large, polydisperse structures devoid of beta gamma complexes and sensitive to disaggregation by guanine nucleotides.

1990 • 45 citations

Glucagon receptor-mediated activation of Gs is accompanied by subunit dissociation.

1988 • 37 citations

Heterogeneity of three electrophoretically distinct G_o α‐subunits in mammalian brain

1992 • 34 citations

A truncated recombinant alpha subunit of Gi3 with a reduced affinity for beta gamma dimers and altered guanosine 5'-3-O-(thio)triphosphate binding.

1992 • 33 citations

Interactions of pure βγ‐subunits of G‐proteins with purified β₁‐adrenoceptor

1988 • 26 citations

Purification of the G-protein G13 from rat brain membranes

1994 • 15 citations

Rapid Isolation of Gα13 from Bovine Brain Membranes: Supportive Effect of Ethylene Glycol

1994 • 3 citations