A truncated recombinant alpha subunit of Gi3 with a reduced affinity for beta gamma dimers and altered guanosine 5'-3-O-(thio)triphosphate binding.
Data up to Jan 2025
Total Citations Per Year
Abstract
References (31)
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
1951 • 319,299 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
1970 • 253,849 citations
Molecular Cloning. A Laboratory Manual
1983 • 27,328 citations
G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS
1987 • 6,374 citations
[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis
1983 • 1,115 citations
Trends in the Development of Baculovirus Expression Vectors
1988 • 1,022 citations
Mechanism of cholera toxin action: Covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system
1978 • 902 citations
Roles of G protein subunits in transmembrane signalling
1988 • 794 citations
Purification and properties of the inhibitory guanine nucleotide regulatory unit of brain adenylate cyclase.
1984 • 585 citations
G Proteins in Signal Transduction
1990 • 479 citations
The α Subunit of the GTP Binding Protein G k Opens Atrial Potassium Channels
1987 • 317 citations
Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins
1983 • 309 citations
Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.
1985 • 286 citations
Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclases. Purification of the human erythrocyte proteins without the use of activating regulatory ligands.
1984 • 242 citations
Catecholamine-stimulated GTPase cycle. Multiple sites of regulation by beta-adrenergic receptor and Mg2+ studied in reconstituted receptor-Gs vesicles.
1986 • 225 citations
Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system
1987 • 151 citations
G protein .beta..gamma. subunits from bovine brain and retina: equivalent catalytic support of ADP-ribosylation of .alpha. subunits by pertussis toxin but differential interactions with Gs.alpha.
1989 • 139 citations
Characterization of transducin from bovine retinal rod outer segments. Participation of the amino-terminal region of T alpha in subunit interaction.
1987 • 131 citations
Subunit interactions of native and ADP-ribosylated alpha 39 and alpha 41, two guanine nucleotide-binding proteins from bovine cerebral cortex.
1986 • 116 citations
Alpha i-3 cDNA encodes the alpha subunit of Gk, the stimulatory G protein of receptor-regulated K+ channels.
1988 • 104 citations
The amino terminus of G protein alpha subunits is required for interaction with beta gamma.
1988 • 104 citations
Gamma-subunits of G proteins, but not their alpha- or beta-subunits, are polyisoprenylated. Studies on post-translational modifications using in vitro translation with rabbit reticulocyte lysates
1991 • 103 citations
GTP hydrolysis by pure Ni, the inhibitory regulatory component of adenylyl cyclases.
1984 • 89 citations
Expression and purification of functional G protein alpha subunits using a baculovirus expression system.
1992 • 85 citations
Recombinant αi-3 Subunit of G Protein Activates Gk-gated K+ Channels
1989 • 79 citations
Distinct guanine nucleotide binding and release properties of the three Gi proteins.
1990 • 75 citations
Deletion within the amino-terminal region of Gs alpha impairs its ability to interact with beta gamma subunits and to activate adenylate cyclase
1991 • 50 citations
Reticulocyte lysates synthesize an active alpha subunit of the stimulatory G protein Gs.
1988 • 48 citations
G protein subunit interactions
1989 • 39 citations
The interaction of nucleotides with pertussis toxin. Direct evidence for a nucleotide binding site on the toxin regulating the rate of ADP-ribosylation of Ni, the inhibitory regulatory component of adenylyl cyclase.
1986 • 39 citations
Urea Gradient/SDS-Page; A Useful Tool in the Investigation of Signal Transducing G Proteins
1991 • 28 citations