Role of nucleotide hydrolysis in the dynamics of actin filaments and microtubules

Dynamic instability of microtubule growth

1984 • 3,187 citations

ACTIN AND ACTIN-BINDING PROTEINS. A CRITICAL EVALUATION OF MECHANISMS AND FUNCTIONS

1986 • 1,493 citations

Beyond self-assembly: From microtubules to morphogenesis

1986 • 1,334 citations

Colchicine-binding protein of mammalian brain and its relation to microtubules

1968 • 1,140 citations

Enzyme-Catalyzed Phosphoryl Transfer Reactions

1980 • 1,120 citations

Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.

1987 • 833 citations

Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments.

1986 • 828 citations

Microtubule assembly nucleated by isolated centrosomes

1984 • 775 citations

Actin polymerization and its regulation by proteins from nonmuscle cells.

1982 • 739 citations

Head to tail polymerization of actin

1976 • 692 citations

Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling.

1985 • 637 citations

Thermodynamics of the polymerization of protein

1975 • 596 citations

Opposite end assembly and disassembly of microtubules at steady state in vitro

1978 • 567 citations

Visualization of the dynamic instability of individual microtubules by dark-field microscopy

1986 • 547 citations

Tubulin dynamics in cultured mammalian cells.

1984 • 489 citations

Actin Polymerization and ATP Hydrolysis

1987 • 439 citations

Posttranslational modification and microtubule stability.

1987 • 365 citations

Microtubule dynamics in interphase cells.

1986 • 331 citations

Phosphate Release and Force Generation in Skeletal Muscle Fibers

1985 • 324 citations

Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.

1987 • 314 citations

Subunits in biological systems

1971 • 299 citations

Direct measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores.

1981 • 298 citations

Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules.

1987 • 297 citations

Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules

1976 • 281 citations

Adenosinetriphosphate the functional group of actin

1950 • 280 citations

Head-to-tail polymerization of microtubules in vitro. Electron microscope analysis of seeded assembly.

1980 • 279 citations

Dynamic and stable populations of microtubules in cells.

1987 • 273 citations

Kinetic analysis of guanosine 5'-triphosphate hydrolysis associated with tubulin polymerization

1981 • 270 citations

Kinetic analysis of microtubule self-assembly in vitro

1977 • 260 citations

The acetylation of alpha-tubulin and its relationship to the assembly and disassembly of microtubules.

1986 • 252 citations

The reversibility of adenosine triphosphate cleavage by myosin

1973 • 249 citations

Bioenergetics and Kinetics of Microtubule and Actin Filament Assembly–Disassembly

1982 • 242 citations

RELATIONSHIPS BETWEEN CHEMICAL AND MECHANICAL EVENTS DURING MUSCULAR CONTRACTION

1986 • 241 citations

Implications of treadmilling for the stability and polarity of actin and tubulin polymers in vivo.

1980 • 232 citations

Mitotic mechanism based on intrinsic microtubule behaviour

1978 • 232 citations

Pathway of the Microtubule-Dynein ATPase and the Structure of Dynein: A Comparison with Actomyosin

1985 • 215 citations

In Vitro Assembly of Cytoplasmic Microtubules

1980 • 189 citations

Kinetic analysis of cooperativity in tubulin polymerization in the presence of guanosine di- or triphosphate nucleotides

1978 • 177 citations

Guanosinetriphosphatase activity of tubulin associated with microtubule assembly.

1977 • 174 citations

Elementary processes of the magnesium ion-dependent adenosine triphosphatase activity of heavy meromysin. A transient kinetic approach to the study of kinases and adenosine triphosphatases and a colorimetric inorganic phosphate assay in situ

1972 • 165 citations

Nucleotide binding and phosphorylation in microtubule assembly in vitro

1977 • 163 citations

Microfilaments: dynamic arrays in higher plant cells

1987 • 162 citations

Interference of GTP hydrolysis in the mechanism of microtubule assembly: an experimental study.

1984 • 160 citations

Direct evidence for ADP-inorganic phosphate-F-actin as the major intermediate in ATP-actin polymerization. Rate of dissociation of inorganic phosphate from actin filaments

1986 • 160 citations

Evidence for an ATP cap at the ends of actin filaments and its regulation of the F-actin steady state.

1984 • 149 citations

Binding of phosphate to F-ADP-actin and role of F-ADP-Pi-actin in ATP-actin polymerization.

1988 • 142 citations

Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes.

1985 • 142 citations

Interpretation of the light scattering from long rods

1974 • 141 citations

Mg2+ dependence of guanine nucleotide binding to tubulin.

1987 • 140 citations

Direct measurement of critical concentrations and assembly rate constants at the two ends of an actin filament

1983 • 139 citations

Direct observation of steady-state microtubule dynamics.

1986 • 139 citations

Mechanism of K+-induced actin assembly.

1982 • 132 citations

The rate constant for ATP hydrolysis by polymerized actin

1984 • 127 citations

Assembly and turnover of detyrosinated tubulin in vivo.

1987 • 123 citations

The synthesis of enzyme-bound ATP by soluble chloroplast coupling factor 1.

1982 • 122 citations

Microtubule dynamics in vivo: a test of mechanisms of turnover.

1987 • 120 citations

Actin and Tubulin Polymerization: The Use of Kinetic Methods to Determine Mechanism

1985 • 119 citations

The structure of F-actin

1985 • 118 citations

GTP hydrolysis during microtubule assembly

1987 • 117 citations

Head-to-tail polymerization of microtubules in vitro

1981 • 115 citations

Monte Carlo study of the GTP cap in a five-start helix model of a microtubule.

1985 • 114 citations

On the mechanism of actin monomer-polymer subunit exchange at steady state.

1983 • 112 citations

Dynamics of microtubule depolymerization in monocytes.

1986 • 109 citations

Interaction of plasma gelsolin with G-actin and F-actin in the presence and absence of calcium ions.

1985 • 105 citations

Synchronous oscillations in microtubule polymerization.

1987 • 104 citations

12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.

1983 • 104 citations

The effects of Mg2+ at the high-affinity and low-affinity sites on the polymerization of actin and associated ATP hydrolysis.

1986 • 101 citations

Microtubule Assembly: A Review of Progress, Principles, and Perspectives

1984 • 101 citations

Phase changes at the end of a microtubule with a GTP cap.

1984 • 96 citations

Guanosine-5?-triphosphate hydrolysis and tubulin polymerization

1982 • 96 citations

Stabilization of microtubules by inorganic phosphate and its structural analogs, the fluoride complexes of aluminum and beryllium

1988 • 96 citations

Effect of villin on the kinetics of actin polymerization

1984 • 95 citations

Characterization and dynamics of cytoplasmic F-actin in higher plant endosperm cells during interphase, mitosis, and cytokinesis.

1987 • 94 citations

Stoichiometry and role of GTP hydrolysis in bovine neurotubule assembly

1978 • 89 citations

Microtubule elongation and guanosine 5'-triphosphate hydrolysis. Role of guanine nucleotides in microtubule dynamics

1987 • 88 citations

Mechanisms of Assembly and Disassembly of Microtubules

1983 • 84 citations

The mechanisms of ATP hydrolysis accompanying the polymerization of Mg-actin and Ca-actin.

1987 • 81 citations

Generation of microtubule stability subclasses by microtubule-associated proteins: implications for the microtubule "dynamic instability" model.

1985 • 81 citations

The critical concentration of actin in the presence of ATP increases with the number concentration of filaments and approaches the critical concentration of actin.ADP.

1984 • 81 citations

Interaction of cytochalasin D with actin filaments in the presence of ADP and ATP.

1986 • 80 citations

A model for actin polymerization and the kinetic effects of ATP hydrolysis.

1985 • 79 citations

Mobility of filamentous actin in living cytoplasm.

1987 • 79 citations

Steady-state theory of the interference of GTP hydrolysis in the mechanism of microtubule assembly.

1983 • 78 citations

Introductory analysis of the GTP-cap phase-change kinetics at the end of a microtubule.

1984 • 76 citations

Actin polymerization. The effect of brevin on filament size and rate of polymerization.

1984 • 74 citations

Exchange of ADP, ATP and 1:N⁶‐Ethenoadenosine 5′‐Triphosphate at G‐Actin

1979 • 73 citations

Interaction of actin with analogs of adenosine triphosphate

1973 • 69 citations

Rate of treadmilling of actin filaments in vitro

1986 • 68 citations

In vivo coassembly of a divergent beta-tubulin subunit (c beta 6) into microtubules of different function.

1987 • 65 citations

Polymerization of ADP-actin and ATP-actin under sonication and characteristics of the ATP-actin equilibrium polymer.

1985 • 65 citations

The hydrolysis of ATP that accompanies actin polymerization is essentially irreversible

1988 • 64 citations

Cytoplasmic concentrations of inorganic phosphate affect the critical concentration for assembly of actin in the presence of cytochalasin D or ADP

1986 • 59 citations

Sequence of chicken cβ7 tubulin

1988 • 56 citations

Role of GTP in the Assembly of Microtubules

1977 • 56 citations

Stress fiber and cleavage furrow formation in living cells microinjected with fluorescently labeled α‐actinin

1987 • 56 citations

Role of co-operative interactions, microtubule-associated proteins and guanosine triphosphate in microtubule assembly: a model.

1980 • 55 citations

Measurement of Pi dissociation from actin filaments following ATP hydrolysis using a linked enzyme assay

1987 • 51 citations

Incorporation of radioactive tubulin into microtubules at steady state. Experimental and theoretical analyses of diffusional and directional flux.

1980 • 51 citations

Dephosphorylation of adenosine triphosphate in actin solutions at low concentrations of magnesium

1960 • 50 citations

Oscillations in microtubule polymerization: the rate of GTP regeneration on tubulin controls the period.

1988 • 50 citations

Theoretical treatment of microtubules disappearing in solution.

1985 • 49 citations

Conformation and assembly characteristics of tubulin and microtubule protein from bovine brain

1981 • 44 citations

Sequential phosphorylation of chartin microtubule-associated proteins is regulated by the presence of microtubules.

1987 • 44 citations

GTPase activity at ends of microtubules

1978 • 43 citations

Thermodynamic analysis of microtubule self-assembly in vitro

1979 • 40 citations

Deoxyguanosine nucleotide analogs: potent stimulators of microtubule nucleation with reduced affinity for the exchangeable nucleotide site of tubulin

1984 • 38 citations

Effect of Phosphates on the Structure of the Actin Filament1

1975 • 37 citations

Effect of actin concentration on the intermediate oxygen exchange of myosin; relation to the refractory state and the mechanism of exchange

1978 • 37 citations

Release of exchangeably bound guanine nucleotides from tubulin in a magnesium-free buffer

1985 • 37 citations

[154] Isolation and properties of the Escherichia coli amino acid polymerizing enzymes

1968 • 35 citations

Theoretical studies on oscillations in microtubule polymerization.

1987 • 34 citations

Differential effects of magnesium on tubulin-nucleotide interactions

1985 • 30 citations

ATP hydrolysis by the gelsolin-actin complex and at the pointed ends of gelsolin-capped filaments.

1986 • 30 citations

Involvement of Guanosine Triphosphate (GTP) Hydrolysis in the Mechanism of Tubulin Polymerization: Regulation of Microtubule Dynamics at Steady State by a GTP Cap

1986 • 29 citations

Role of nucleotide hydrolysis in the polymerization of actin and tubulin

1988 • 29 citations

Steady-state head-to-tail polymerization of actin or microtubules. II. Two-state and three-state kinetic cycles

1981 • 29 citations

Nonlinear increase of elongation rate of actin filaments with actin monomer concentration

1986 • 29 citations

Effects of pH on tubulin-nucleotide interactions

1986 • 27 citations

Theoretical study of a model for the ATP cap at the end of an actin filament

1986 • 26 citations

On the nonlinear relationship between the initial rates of dilution-induced microtubule disassembly and the initial free subunit concentration.

1983 • 26 citations

Binding of phosphate ions to actin

1987 • 26 citations

Direct incorporation of guanosine 5-diphosphate into microtubules without guanosine 5'-triphosphate hydrolysis

1986 • 24 citations

On the relationship between nucleotide hydrolysis and microtubule asssembly : Studies with a GTP-regenerating system

1987 • 24 citations

The rate of ATP synthesis by dynein

1986 • 23 citations

Interaction of plasma gelsolin with ADP-actin.

1986 • 21 citations

Kinetics of actin elongation and depolymerization at the pointed end

1987 • 21 citations

Phase-change kinetics for a microtubule with two free ends.

1985 • 21 citations

Mean lifetime of microtubules attached to nucleating sites.

1988 • 15 citations

Kinetic and Steady State Analysis of Microtubule Assembly

1986 • 13 citations

Effects of GDP on microtubules at steady state

1987 • 6 citations

Actin: High-resolution electron microscopy on its subunit and filamentous structure, and its molecular interactions

1987 • 2 citations