Prevalence of a common point mutation in the dihydropyrimidine dehydrogenase (DPD) gene within the 5'-splice donor site of intron 14 in patients with severe 5-fluorouracil (5-FU)- related toxicity compared with controls.

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Clinical pharmacokinetics of 5-fluorouracil and its metabolites in plasma, urine, and bile.

1987 • 720 citations

Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples

1999 • 664 citations

Familial deficiency of dihydropyrimidine dehydrogenase. Biochemical basis for familial pyrimidinemia and severe 5-fluorouracil-induced toxicity.

1988 • 500 citations

Molecular basis of the human dihydropyrimidine dehydrogenase deficiency and 5-fluorouracil toxicity.

1996 • 365 citations

Clinical implications of dihydropyrimidine dehydrogenase (DPD) deficiency in patients with severe 5-fluorouracil-associated toxicity: identification of new mutations in the DPD gene.

2000 • 353 citations

Quantitation of Dihydropyrimidine Dehydrogenase Expression by Real-Time Reverse Transcription Polymerase Chain Reaction

2000 • 345 citations

Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species.

1993 • 319 citations

Genotype and phenotype in patients with dihydropyrimidine dehydrogenase deficiency

1999 • 272 citations

Optimisation of DNA and RNA extraction from archival formalin-fixed tissue

1999 • 257 citations

Familial Pyrimidinemia and Pyrimidinuria Associated with Severe Fluorouracil Toxicity

1985 • 254 citations

Correlation between dihydropyrimidine dehydrogenase activity in peripheral mononuclear cells and systemic clearance of fluorouracil in cancer patients.

1992 • 233 citations

Severe 5-fluorouracil toxicity secondary to dihydropyrimidine dehydrogenase deficiency. A potentially more common pharmacogenetic syndrome

1991 • 225 citations

Life-threatening toxicity in a dihydropyrimidine dehydrogenase-deficient patient after treatment with topical 5-fluorouracil.

1999 • 223 citations

Known variant DPYD alleles do not explain DPD deficiency in cancer patients

2000 • 184 citations

Characterization of the Human Dihydropyrimidine Dehydrogenase Gene

1998 • 167 citations

Severe neurotoxicity following 5-fluorouracil-based chemotherapy in a patient with dihydropyrimidine dehydrogenase deficiency.

1996 • 164 citations

Human Polymorphism in Drug Metabolism: Mutation in the Dihydropyrimidine Dehydrogenase Gene Results in Exon Skipping and Thymine Uracilurea

1995 • 135 citations

Dihydropyrimidine dehydrogenase pharmacogenetics in Caucasian subjects

1998 • 126 citations

cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.

1994 • 121 citations

Heterozygosity for a point mutation in an invariant splice donor site of dihydropyrimidine dehydrogenase and severe 5-fluorouracil related toxicity

1997 • 110 citations

A point mutation in an invariant splice donor site leads to exon skipping in two unrelated Dutch patients with dihydropyrimidine dehydrogenase deficiency

1996 • 101 citations

Dihydropyrimidine dehydrogenase pharmacogenetics in patients with colorectal cancer

1998 • 92 citations

Identification of novel mutations in the dihydropyrimidine dehydrogenase gene in a Japanese patient with 5-fluorouracil toxicity.

1998 • 73 citations

Structural organization of the human dihydropyrimidine dehydrogenase gene.

1997 • 67 citations

Putative Role of Dihydropyrimidine Dehydrogenase in the Toxic Side Effect of 5-Fluorouracil in Colorectal Cancer Patients

1998 • 52 citations

5-Fluorouracil Pharmacokinetics: Causes for Variability and Strategies for Modulation in Cancer Chemotherapy

1999 • 44 citations

Mutations at codon 974 of the DPYD gene are a rare event

1997 • 9 citations