Signal transduction by tumor necrosis factor alpha is mediated through a guanine nucleotide-binding protein in osteoblast-like cell line, MC3T3-E1.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Phosphorus Assay in Column Chromatography

1959 • 12,895 citations

An endotoxin-induced serum factor that causes necrosis of tumors.

1975 • 4,522 citations

In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.

1983 • 1,661 citations

Recombinant Human Tumor Necrosis Factor-α: Effects on Proliferation of Normal and Transformed Cells in Vitro

1985 • 1,596 citations

Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors

1986 • 1,433 citations

Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin

1986 • 1,264 citations

Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts.

1985 • 1,240 citations

Molecular cloning and expression of a receptor for human tumor necrosis factor

1990 • 973 citations

Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor

1990 • 918 citations

Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

1986 • 917 citations

Roles of G protein subunits in transmembrane signalling

1988 • 794 citations

Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors.

1986 • 674 citations

Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells.

1990 • 484 citations

Human tumor necrosis factor produced by human B-cell lines: synergistic cytotoxic interaction with human interferon.

1983 • 458 citations

Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells.

1985 • 430 citations

Inositol trisphosphate-induced calcium release and contraction in vascular smooth muscle.

1985 • 417 citations

Loss of the inhibitory function of the guanine nucleotide regulatory component of adenylate cyclase due to its ADP ribosylation by islet-activating protein, pertussis toxin, in adipocyte membranes.

1983 • 406 citations

Signal transmission by the insulin-like growth factors

1989 • 380 citations

Cytosolic heparin inhibits muscarinic and α-adrenergic Ca2+ release in smooth muscle

1989 • 344 citations

Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.

1982 • 323 citations

Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity.

1985 • 287 citations

Receptor-mediated inhibition of adenylate cyclase and stimulation of arachidonic acid release in 3T3 fibroblasts. Selective susceptibility to islet-activating protein, pertussis toxin.

1985 • 245 citations

Guanine nucleotides modulate the binding affinity of the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes.

1983 • 223 citations

Binding of human tumor necrosis factor to high affinity receptors on HeLa and lymphoblastoid cells sensitive to growth inhibition.

1985 • 183 citations

Development of a novel, Ins(1,4,5)P3-specific binding assay: Its use to determine the intracellular concentration of Ins(1,4,5)P3 in unstimulated and vasopressin-stimulated rat hepatocytes

1989 • 182 citations

Stimulation of prostaglandin E2 synthesis in cloned osteoblastic cells of mouse (MC3T3-E1) by epidermal growth factor.

1986 • 167 citations

Differences and similarities in the noradrenaline‐ and caffeine‐induced mechanical responses in the rabbit mesenteric artery

1983 • 146 citations

Cloning of human tumor necrosis factor (TNF) receptor cDNA and expression of recombinant soluble TNF-binding protein.

1990 • 141 citations

Epidermal growth factor stimulation of prostaglandin E2 biosynthesis in amnion cells. Induction of prostaglandin H2 synthase.

1988 • 135 citations

Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes.

1988 • 107 citations

Effect of tumor necrosis factor on GTP binding and GTPase activity in HL-60 and L929 cells.

1988 • 101 citations

A Permissive Role of Pertussis Toxin Substrate G-protein in P2-purinergic Stimulation of Phosphoinositide Turnover and Arachidonate Release in FRTL-5 Thyroid Cells

1989 • 99 citations

Evidence of GTP-binding protein regulation of phospholipase A2 activity in isolated human platelet membranes

1989 • 64 citations

Possible induction of fatty acid cyclooxygenase in mouse osteoblastic cells (MC3T3-E1) by cAMP

1988 • 48 citations

Pertussis toxin inhibits the action of insulin-like growth factor-I

1987 • 46 citations

Evidence for coupling of bradykinin receptors to a guanine-nucleotide binding protein to stimulate arachidonate liberation in the osteoblast-like cell line, MC3T3-E1

1991 • 46 citations

Effects of human recombinant tumor necrosis factor-alpha and interleukin 1 on the synthesis of glycosaminoglycan and DNA in cultured rat costal chondrocytes.

1988 • 45 citations

Mitogenic signalling pathway of tumour necrosis factor involves the rapid tyrosine phosphorylation of 41,000-Mr and 43,000-Mr cytosol proteins

1990 • 44 citations

Fatty acid cyclooxygenase activity stimulated by transforming growth factor-β in mouse osteoblastic cells (MC3T3-E1)

1989 • 43 citations

Insulin receptor function is inhibited by guanosine 5′-[γ-thio]triphosphate (GTP[S])

1990 • 24 citations

Origin of intracellular calcium and quantitation of mobilizable calcium in neutrophils stimulated with chemotactic peptide

1986 • 22 citations

Changes of Intracellular Free Ca2+ in Macrophages Following N-Formyl Chemotactic Peptide Stimulation. Direct Measurement by the Loading of Quin 21

1984 • 19 citations