Linker insertion-deletion mutagenesis of the v-src gene: isolation of host- and temperature-dependent mutants

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Measurement of protein using bicinchoninic acid

1985 • 17,516 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

A novel viral oncogene with structural similarity to phospholipase C

1988 • 789 citations

Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors

1987 • 651 citations

Sequence similarity of phospholipase C with the non-catalytic region of src

1988 • 541 citations

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

1984 • 481 citations

A Noncatalytic Domain Conserved among Cytoplasmic Protein-Tyrosine Kinases Modifies the Kinase Function and Transforming Activity of Fujinami Sarcoma Virus P130^gag-fps

1986 • 478 citations

A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.

1984 • 447 citations

Cell Transformation by the Viral src Oncogene

1987 • 405 citations

Aligning amino acid sequences: Comparison of commonly used methods

1985 • 400 citations

Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.

1985 • 386 citations

Molecular cloning and characterization of avian sarcoma virus circular DNA molecules

1980 • 361 citations

Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.

1980 • 334 citations

Normal rat cell lines deficient in nuclear thymidine kinase

1981 • 307 citations

Interaction of the Rous Sarcoma Virus Protein pp60 src with the Cellular Proteins pp50 and pp90

1986 • 223 citations

Rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation

1986 • 223 citations

New Procedure for DNA Transfection with Polycation and Dimethyl Sulfoxide

1984 • 217 citations

Local mutagenesis of Rous sarcoma virus: The major sites of tyrosine and serine phosphorylation of p60src are dispensable for transformation

1983 • 189 citations

Regulation of Cell Growth and Transformation by Tyrosine-Specific Protein Kinases: The Search for Important Cellular Substrate Proteins

1983 • 164 citations

A Short Sequence in the p60^src N Terminus Is Required for p60^src Myristylation and Membrane Association and for Cell Transformation

1984 • 149 citations

Phosphorylation of tyrosine-416 is not required for the transforming properties and kinase activity of pp60v-src

1983 • 138 citations

The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: lysine 7 is a critical determinant.

1988 • 136 citations

Mutagenesis of the region between env and src of the SR-A strain of rous sarcoma virus for the purpose of constructing helper-independent vectors

1984 • 134 citations

Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis

1984 • 113 citations

A conserved domain regulates interactions of the v-fps protein-tyrosine kinase with the host cell.

1987 • 89 citations

N-terminal deletions in Rous sarcoma virus p60src: effects on tyrosine kinase and biological activities and on recombination in tissue culture with the cellular src gene.

1985 • 88 citations

Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation

1982 • 83 citations

Amino Acid Alterations Within a Highly Conserved Region of the Rous Sarcoma Virus src Gene Product pp60^src Inactivate Tyrosine Protein Kinase Activity

1984 • 75 citations

Mutation of a termination codon affects src initiation.

1984 • 68 citations

Phosphotyrosine-containing proteins and expression of transformation parameters in cells infected with partial transformation mutants of Rous sarcoma virus

1983 • 62 citations

Genetic analysis of p60v-src domains involved in the induction of different cell transformation parameters

1986 • 58 citations

The First Seven Amino Acids Encoded by the v-src Oncogene Act as a Myristylation Signal: Lysine 7 is a Critical Determinant

1988 • 48 citations

Features of the pp60^v-src Carboxyl Terminus That Are Required for Transformation

1986 • 47 citations

Genetic analysis of the form and function of the viral src oncogene product

1987 • 47 citations

N-Terminal Deletions in Rous Sarcoma Virus p60^src: Effects on Tyrosine Kinase and Biological Activities and on Recombination in Tissue Culture with the Cellular src Gene

1985 • 44 citations

Identification of an amino terminal domain required for the transforming activity of the Rous sarcoma virus src protein

1987 • 41 citations

Mutation of a Termination Codon Affects src Initiation

1984 • 40 citations

Structural domains of the avian erythroblastosis virus erbB protein required for fibroblast transformation: dissection by in-frame insertional mutagenesis

1986 • 33 citations

Homology Among Oncogenes

1986 • 26 citations

Molecular cloning of the intronless EJ ras oncogene using a murine retrovirus shuttle vector

1986 • 9 citations

A host-dependent temperature-sensitive mutant of rous sarcoma virus: Evidence for host factors affecting transformation

1988 • 6 citations

Isolation of a new plasmid pIRL19: its use in construction of small vectors and detection of specific sequence in a foreign DNA

1983 • 2 citations