Regulation of Phosphatidylinositol 3′-Kinase by Tyrosyl Phosphoproteins

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Oncogenes and signal transduction

1991 • 2,816 citations

Phosphatidylinositol-3-OH kinase direct target of Ras

1994 • 1,923 citations

Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein

1991 • 1,590 citations

Phosphatidylinositol 3′-kinase is activated by association with IRS-1 during insulin stimulation.

1992 • 1,030 citations

Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate

1988 • 1,027 citations

PDGF-dependent tyrosine phosphorylation stimulates production of novel polyphosphoinositides in intact cells

1989 • 933 citations

SH2 and SH3 domains

1993 • 697 citations

Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases

1991 • 641 citations

Activation of phosphatidylinositol 3-kinase by insulin.

1990 • 531 citations

Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins

1989 • 524 citations

IRS-1 activates phosphatidylinositol 3'-kinase by associating with src homology 2 domains of p85.

1992 • 471 citations

Pleiotropic insulin signals are engaged by multisite phosphorylation of IRS-1.

1993 • 452 citations

PI 3-kinase is a dual specificity enzyme: autoregulation by an intrinsic protein-serine kinase activity.

1994 • 450 citations

Activation of Phosphatidylinositol-3′ Kinase by Src-Family Kinase SH3 Binding to the p85 Subunit

1994 • 437 citations

Phosphoinositide 3-kinase is activated by phosphopeptides that bind to the SH2 domains of the 85-kDa subunit

1993 • 395 citations

PI 3-kinase: structural and functional analysis of intersubunit interactions.

1994 • 329 citations

Interaction of phosphatidylinositol 3-kinase-associated p85 with epidermal growth factor and platelet-derived growth factor receptors.

1992 • 319 citations

Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo.

1992 • 317 citations

Point mutations in the abl SH2 domain coordinately impair phosphotyrosine binding in vitro and transforming activity in vivo.

1992 • 295 citations

Phosphorylation of the PDGF receptor beta subunit creates a tight binding site for phosphatidylinositol 3 kinase.

1990 • 244 citations

Tyr721 regulates specific binding of the CSF-1 receptor kinase insert to PI 3′-kinase SH2 domains: a model for SH2-mediated receptor-target interactions.

1992 • 241 citations

A Tightly Associated Serine/Threonine Protein Kinase Regulates Phosphoinositide 3-Kinase Activity

1993 • 199 citations

A novel recognition motif for phosphatidylinositol 3-kinase binding mediates its association with the hepatocyte growth factor/scatter factor receptor.

1993 • 173 citations

Association of IRS-1 with the insulin receptor and the phosphatidylinositol 3'-kinase. Formation of binary and ternary signaling complexes in intact cells.

1993 • 160 citations

Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes.

1992 • 132 citations

Direct activation of the phosphatidylinositol 3'-kinase by the insulin receptor.

1994 • 126 citations

Synthesis, purification, and characterization of the cytoplasmic domain of the human insulin receptor using a baculovirus expression system.

1988 • 120 citations

Tyrosine mutations within the alpha platelet-derived growth factor receptor kinase insert domain abrogate receptor-associated phosphatidylinositol-3 kinase activity without affecting mitogenic or chemotactic signal transduction.

1991 • 109 citations

Phosphatidylinositol 3-kinase: a novel effector.

1992 • 99 citations

A region of the 85-kilodalton (kDa) subunit of phosphatidylinositol 3-kinase binds the 110-kDa catalytic subunit in vivo.

1993 • 98 citations

Phosphatidylinositol 3-kinase activation is mediated by high-affinity interactions between distinct domains within the p110 and p85 subunits

1994 • 90 citations

Transformation-defective mutants of polyomavirus middle T antigen associate with phosphatidylinositol 3-kinase (PI 3-kinase) but are unable to maintain wild-type levels of PI 3-kinase products in intact cells

1992 • 67 citations

Direct association of p110 beta phosphatidylinositol 3-kinase with p85 is mediated by an N-terminal fragment of p110 beta.

1994 • 61 citations

Potent Activation of Phosphatidylinositol 3'-Kinase by Simple Phosphotyrosine Peptides Derived from Insulin Receptor Substrate 1 Containing Two YMXM Motifs for binding SH2 Domains

1994 • 48 citations