Characterization of the Cephalosporium acremonium pcbAB gene encoding alpha-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Sequences important for gene expression in filamentous fungi

1986 • 405 citations

ORGANIZATION AND EXPRESSION OF GENES INVOLVED IN THE BIOSYNTHESIS OF ANTIBIOTICS AND OTHER SECONDARY METABOLITES

1989 • 327 citations

Attempts to Map a Process Evolution of Peptide Biosynthesis

1971 • 262 citations

The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes.

1990 • 240 citations

Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium

1985 • 225 citations

Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum

1986 • 177 citations

Biosynthesis of Peptide Antibiotics

1987 • 172 citations

[5] Kilo-sequencing: Creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13

1983 • 169 citations

δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine Synthetase from Aspergillus nidulans

1989 • 168 citations

Cloning and Expression of the Fungal Expandase/hydroxylase Gene Involved in Cephalosporin Biosynthesis

1987 • 164 citations

High–Frequency Transformation of Penicillium Chrysogenum

1987 • 143 citations

The Aspergillus nidulans npeA locus consists of three contiguous genes required for penicillin biosynthesis.

1990 • 140 citations

δ -(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase): a multifunctional enzyme with broad substrate specificity for the synthesis of penicillin and cephalosporin precursors

1987 • 114 citations

An electrophoretic molecular karyotype for an industrial strain of Cephalosporium acremonium

1989 • 94 citations

Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78

1989 • 89 citations

Enzymes involved in Penicillin, cephalosporin and cephamycin biosynthesis

1989 • 87 citations

Synthesis of δ-(α-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis

1976 • 85 citations

BIOSYNTHESIS OF POLYENE MACROLIDE ANTIBIOTICS

1977 • 81 citations

Fatty acid biosynthesis in yeast

1978 • 79 citations

Gene cluster containing the genes for tyrocidine synthetases 1 and 2 from Bacillus brevis: evidence for an operon

1989 • 70 citations

Cell-free synthesis of δ-(L-α-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis

1986 • 68 citations

Complete nucleotide sequence of theE. coliglutathione synthetase gsh-II

1984 • 67 citations

The nudeotide sequence of the gene for γ-glutamylcysteine synthetase ofEscherichia coli

1986 • 65 citations

Cloning, characterization of the acyl-CoA : 6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage to the isopenicillin N synthase gene

1990 • 63 citations

Molecular cloning and sequencing of a cDNA encoding the thioesterase domain of the rat fatty acid synthetase.

1988 • 58 citations

Organization of the biosynthesis genes for the peptide antibiotic gramicidin S

1988 • 52 citations

Molecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA.

1988 • 51 citations

Two genes involved in penicillin biosynthesis are linked in a 5.1 kb SalI fragment in the genome of Penicillium chrysogenum

1989 • 48 citations

Production of the penicillin precursor δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) by cell-free extracts fromStreptomyces clavuligerus

1988 • 47 citations

Coordinate increase of isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase in a high cephalosporin-producing mutant ofAcremonium chrysogenumand simultaneous loss of the three enzymes in a non-producing mutant

1986 • 38 citations

Primary structure of a chymotryptic peptide containing the “active serine” of the thioesterase domain of fatty acid synthase

1981 • 36 citations

Characterization of a loss-of-function mutation in the isopenicillin N synthetase gene of Acremonium chrysogenum

1989 • 31 citations

Amino acid sequence of the serine active‐site region of the medium‐chain S‐acyl fatty acid synthetase thioester hydrolase from rat mammary gland

1987 • 31 citations

Accumulation of tripeptide derivatives by mutants of Cephalosporium acremonium.

1979 • 30 citations

Accumulation of Tripeptide Derivatives by Mutants ofCephalosporium acremonium

1979 • 22 citations

Amino acid sequence around the reactive serine residue of the thioesterase domain of rabbit fatty acid synthase

1985 • 18 citations

Isolation and nature of intracellular alpha-aminoadipic acid-containing peptides from Paecilomyces persicinus P-10

1979 • 16 citations