Monoclonal Antibodies Mimic Insulin Activation of Ribosomal Protein S6 Kinase without Activation of Insulin Receptor Tyrosine Kinase

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes

1985 • 2,257 citations

The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling

1985 • 1,485 citations

[42] Detection and quantification of phosphotyrosine in proteins

1983 • 1,184 citations

Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose

1986 • 1,100 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II

1988 • 1,032 citations

Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin.

1987 • 648 citations

Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.

1987 • 604 citations

A cascade of tyrosine autophosphorylation in the beta-subunit activates the phosphotransferase of the insulin receptor.

1988 • 467 citations

The Molecular Mechanism of Insulin Action

1985 • 424 citations

Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.

1988 • 404 citations

A mutant insulin receptor with defective tyrosine kinase displays no biologic activity and does not undergo endocytosis.

1987 • 403 citations

Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity.

1987 • 399 citations

The effect of serum, EGF, PGF2α and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis

1982 • 362 citations

The Nature and Regulation of the Insulin Receptor: Structure and Function

1985 • 318 citations

Expression and characterization of a functional human insulin-like growth factor I receptor.

1988 • 313 citations

The Insulin Receptor: Molecular Biology and Transmembrane Signaling*

1987 • 285 citations

Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.

1988 • 257 citations

Role of Insulin in the Regulation of Protein Synthesis

1980 • 234 citations

Identification and characterization of a mitogen-activated S6 kinase.

1988 • 223 citations

Acute insulin action requires insulin receptor kinase activity: introduction of an inhibitory monoclonal antibody into mammalian cells blocks the rapid effects of insulin.

1987 • 221 citations

Molecular basis of insulin action

1986 • 201 citations

Insulin receptor signaling. Activation of multiple serine kinases.

1988 • 197 citations

Autophosphorylation of the insulin receptor in vitro. Designation of phosphorylation sites and correlation with receptor kinase activation.

1986 • 158 citations

Activation of S6 kinase activity in 3T3-L1 cells by insulin and phorbol ester.

1985 • 155 citations

Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.

1982 • 151 citations

High-level expression of human insulin receptor cDNA in mouse NIH 3T3 cells.

1987 • 151 citations

Predominance of tyrosine phosphorylation of insulin receptors during the initial response of intact cells to insulin.

1985 • 146 citations

Identification of the insulin receptor tyrosine residues undergoing insulin-stimulated phosphorylation in intact rat hepatoma cells.

1988 • 145 citations

Preferential Utilization of Phosphorylated 40‐S Ribosomal Subunits during Initiation Complex Formation

1982 • 139 citations

Characterization of an endogenous substrate of the insulin receptor in cultured cells.

1987 • 136 citations

Regulation of 40S ribosomal protein S6 phosphorylation in Swiss mouse 3T3 cells

1980 • 125 citations

Epidermal growth factor-mediated activation of an S6 kinase in Swiss mouse 3T3 cells.

1985 • 119 citations

Effect of monoclonal antibodies on human insulin receptor autophosphorylation, negative cooperativity, and down-regulation.

1987 • 110 citations

Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6.

1983 • 106 citations

Phosphorylation of ribosomal protein S6 by cAMP-dependent protein kinase and mitogen-stimulated S6 kinase differentially alters translation of globin mRNA.

1987 • 102 citations

Fibroblast growth factor treatment of Swiss 3T3 cells activates a subunit S6 kinase that phosphorylates a synthetic peptide substrate.

1986 • 96 citations

Monoclonal antibodies to the human insulin receptor that activate glucose transport but not insulin receptor kinase activity.

1987 • 89 citations

Regulation of Protein Synthesis by Phosphorylation of Ribosomal Protein S6 and Aminoacyl-†RNA Synthetases1

1986 • 82 citations

Mapping surface structures of the human insulin receptor with monoclonal antibodies: localization of main immunogenic regions to the receptor kinase domain

1986 • 81 citations

Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase

1989 • 76 citations

Conformational Changes in the α- and β-Subunits of the Insulin Receptor Identified by Anti-peptide Antibodies

1989 • 74 citations

Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.

1987 • 74 citations

Purification of a bovine liver S6 kinase

1987 • 71 citations

Differential regulation of S6 phosphorylation by insulin and epidermal growth factor in Swiss mouse 3T3 cells: insulin activation of type 1 phosphatase.

1988 • 67 citations

Insulin Action in Cultured HTC and H35 Rat Hepatoma Cells: Receptor Binding and Hormone Sensitivity*

1981 • 57 citations

Insulin-induced tyrosine-phosphorylation in intact rat adipocytes

1988 • 57 citations

Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins SI, S3/3a, S6 and S11 within the 48S pre-initiation complex

1984 • 55 citations

Mechanism of insulin action

1987 • 52 citations

Ribosomal Proteins Cross-Linked to Natural mRNA by UV Irradiation of Rat Liver Polysomes1

1981 • 49 citations

Human insulin receptor radioimmunoassay: applicability to insulin-resistant states

1989 • 40 citations

The insulin receptor and tyrosine protein kinase activity

1984 • 32 citations

Reversal of insulin-induced negative cooperativity by monoclonal antibodies that stabilize the slowly dissociating (“Ksuper”) state of the insulin receptor

1988 • 29 citations

Insulin‐stimulated protein synthesis in adipocytes

1985 • 27 citations

Does insulin exert certain of its actions through a distinct species of guanine nucleotide regulatory protein?

1984 • 11 citations

Insulin and Ribosomal Protein S6 Kinase in Rat Pancreatic Acini

1989 • 6 citations