Harnessing Syk Family Tyrosine Kinases as Signaling Domains for Chimeric Single Chain of the Variable Domain Receptors: Optimal Design for T Cell Activation

Specific activation and targeting of cytotoxic lymphocytes through chimeric single chains consisting of antibody-binding domains and the gamma or zeta subunits of the immunoglobulin and T-cell receptors.

1993 • 1,433 citations

Expression of immunoglobulin-T-cell receptor chimeric molecules as functional receptors with antibody-type specificity.

1989 • 1,350 citations

Alterations in Signal Transduction Molecules in T Lymphocytes from Tumor-Bearing Mice

1992 • 650 citations

Partial T cell signaling: Altered phospho-? and lack of zap70 recruitment in APL-induced T cell anergy

1994 • 608 citations

ζ Phosphorylation Without ZAP-70 Activation Induced by TCR Antagonists or Partial Agonists

1995 • 510 citations

Molecular cloning of a porcine gene syk that encodes a 72-kDa protein-tyrosine kinase showing high susceptibility to proteolysis

1991 • 449 citations

Blocked Signal Transduction to the ERK and JNK Protein Kinases in Anergic CD4 ⁺ T Cells

1996 • 438 citations

The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein.

1991 • 415 citations

Loss of T-cell receptor zeta chain and p56lck in T-cells infiltrating human renal cell carcinoma.

1993 • 385 citations

Blocked Ras Activation in Anergic CD4 ⁺ T Cells

1996 • 380 citations

T cell activation by clustered tyrosine kinases

1993 • 379 citations

Decreased expression of the signal-transducing zeta chains in tumor-infiltrating T-cells and NK cells of patients with colorectal carcinoma.

1993 • 344 citations

Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 Å resolution

1992 • 268 citations

In vivo antitumor activity of T cells redirected with chimeric antibody/T-cell receptor genes.

1995 • 261 citations

Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling.

1994 • 248 citations

Lysis of ovarian cancer cells by human lymphocytes redirected with a chimeric gene composed of an antibody variable region and the Fc receptor gamma chain.

1993 • 242 citations

Fc epsilon RI-mediated tyrosine phosphorylation and activation of the 72-kDa protein-tyrosine kinase, PTK72, in RBL-2H3 rat tumor mast cells.

1992 • 231 citations

Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family.

1990 • 225 citations

B lymphocyte activation is accompanied by phosphorylation of a 72-kDa protein-tyrosine kinase

1991 • 204 citations

Targeting of T lymphocytes to Neu/HER2-expressing cells using chimeric single chain Fv receptors.

1993 • 203 citations

Cross-linking of Fc gamma receptor I (Fc gamma RI) and receptor II (Fc gamma RII) on monocytic cells activates a signal transduction pathway common to both Fc receptors that involves the stimulation of p72 Syk protein tyrosine kinase.

1993 • 198 citations

Accessory cell independent proliferation of human T4 cells stimulated by immobilized monoclonal antibodies to CD3.

1987 • 188 citations

Molecular cloning and chromosomal localization of the human T-cell receptor zeta chain: distinction from the molecular CD3 complex.

1988 • 180 citations

Differential Intrinsic Enzymatic Activity of Syk and Zap-70 Protein-tyrosine Kinases

1996 • 179 citations

Two-step TCRζ/CD3-CD4 and CD28 signaling in T cells: SH2/SH3 domains, protein-tyrosine and lipid kinases

1994 • 176 citations

Generation of effector T cells expressing chimeric T cell receptor with antibody type-specificity.

1989 • 147 citations

A tyrosine-phosphorylated 70-kDa protein binds a photoaffinity analogue of ATP and associates with both the zeta chain and CD3 components of the activated T cell antigen receptor.

1992 • 146 citations

Involvement of p72syk, a protein-tyrosine kinase, in Fc gamma receptor signaling

1993 • 137 citations

Association of tyrosine protein kinase Zap-70 with the protooncogene product p120c-cbl in T lymphocytes.

1996 • 130 citations

Sos, Vav, and C3G Participate in B Cell Receptor-induced Signaling Pathways and Differentially Associate with Shc-Grb2, Crk, and Crk-L Adaptors

1996 • 118 citations

Stimulation of macrophage Fc gamma RIIIA activates the receptor-associated protein tyrosine kinase Syk and induces phosphorylation of multiple proteins including p95Vav and p62/GAP-associated protein.

1994 • 117 citations

p56lck-independent activation and tyrosine phosphorylation of p72syk by T-cell antigen receptor/CD3 stimulation.

1994 • 114 citations

Clustering of the platelet Fc gamma receptor induces noncovalent association with the tyrosine kinase p72syk.

1994 • 105 citations

A spacer region between the single chain antibody- and the CD3 zeta-chain domain of chimeric T cell receptor components is required for efficient ligand binding and signaling activity.

1995 • 99 citations

The CD8 coreceptor revisited: One chain good, two chains better

1994 • 99 citations

T cell antigen receptor signaling: Three families of tyrosine kinases and a phosphatase

1994 • 92 citations

Differential association of protein tyrosine kinases with the T cell receptor is linked to the induction of anergy and its prevention by B7 family-mediated costimulation.

1996 • 85 citations

Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.

1994 • 82 citations

Activation-dependent phosphorylation of the T-lymphocyte surface receptor CD28 and associated proteins.

1994 • 81 citations

Preventing abnormalities in signal transduction of T cells in cancer: the promise of cytokine gene therapy

1996 • 77 citations

T cells infiltrating non-Hodgkin's B cell lymphomas show altered tyrosine phosphorylation pattern even though T cell receptor/CD3-associated kinases are present.

1995 • 76 citations

New simplified molecular design for functional T cell receptor

1993 • 76 citations

Crk Interacts with Tyrosine-phosphorylated p116 upon T Cell Activation

1995 • 72 citations

A structural view of CD4 and CD8

1995 • 67 citations

The hinge region of the CD8α chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains

1992 • 61 citations

Growth retardation of tumors by adoptive transfer of cytotoxic T lymphocytes reprogrammed by CD44V6-specific SCFV: ζ-chimera

1996 • 57 citations

Physical and functional interactions of protein tyrosine kinases, p59fyn and ZAP-70, in T cell signaling.

1996 • 46 citations

Direct T Cell Activation by Chimeric Single Chain Fv-Syk Promotes Syk-Cbl Association and Cbl Phosphorylation

1997 • 34 citations

The alpha-chain of murine CD8 lacks an invariant Ig-like disulfide bond but contains a unique intrachain loop instead.

1989 • 22 citations

Specific elimination of IgE production using T cell lines expressing chimeric T cell receptor genes

1995 • 17 citations

Redirected Cytotoxic Effector Function

1996 • 14 citations

T-Bodies: Chimeric T-Cell Receptors with Antibody-Type Specificity

1995 • 11 citations

Use of bispecific heteroconjugated antibodies (anti-T cell antigen receptor x anti-MHC class II) to study activation of T cells with a full length or truncated antigen receptor zeta-chain.

1993 • 10 citations