Probing the Determinants of Protein Folding and Stability with Amino Acid Substitutions

Some Factors in the Interpretation of Protein Denaturation

1959 • 4,817 citations

Stability of Proteins Small Globular Proteins

1979 • 2,139 citations

Protein Denaturation

1968 • 2,108 citations

Enthalpy–entropy compensation phenomena in water solutions of proteins and small molecules: A ubiquitous properly of water

1970 • 1,173 citations

Protein stability curves

1987 • 990 citations

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

1987 • 738 citations

The Stability of Globular Protein

1975 • 648 citations

Contribution of hydrophobic interactions to protein stability

1988 • 413 citations

Solvent denaturation

1978 • 383 citations

Hydrophobic stabilization in T4 lysozyme determined directly by multiple substitutions of Ile 3

1988 • 375 citations

Validity of the “two‐state” hypothesis for conformational transitions of proteins

1966 • 363 citations

Combinatorial Cassette Mutagenesis as a Probe of the Informational Content of Protein Sequences

1988 • 326 citations

Disulfide Bond Engineered into T4 Lysozyme: Stabilization of the Protein Toward Thermal Inactivation

1984 • 311 citations

Contributions of hydrogen bonds of Thr 157 to the thermodynamic stability of phage T4 lysozyme

1987 • 300 citations

Why does ribonuclease irreversibly inactivate at high temperatures?

1986 • 277 citations

Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation

1986 • 275 citations

Temperature-sensitive mutations of bacteriophage T4 lysozyme occur at sites with low mobility and low solvent accessibility in the folded protein

1987 • 253 citations

Residual structure in large fragments of staphylococcal nuclease: effects of amino acid substitutions

1989 • 234 citations

Genetic and structural analysis of the protein stability problem

1987 • 227 citations

Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond

1987 • 224 citations

Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor.

1984 • 153 citations

Thermodynamic stability and point mutations of bacteriophage T4 lysozyme

1984 • 149 citations

An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of .lambda. repressor

1986 • 149 citations

Stability mutants of staphylococcal nuclease: large compensating enthalpy-entropy changes for the reversible denaturation reaction

1988 • 149 citations

An engineered disulfide bond in dihydrofolate reductase

1987 • 133 citations

Replacements of Pro ⁸⁶ in Phage T4 Lysozyme Extend an α-Helix But Do Not Alter Protein Stability

1988 • 131 citations

Statistical mechanics of noncovalent bonds in polyamino acids. VIII. Covalent loops in proteins

1965 • 130 citations

Stabilization of λ repressor against thermal denaturation by site‐directed Gly→Ala changes in α‐helix 3

1986 • 115 citations

Reversible Denaturation of Sperm Whale Myoglobin. II. Thermodynamic Analysis

1967 • 90 citations

Guanidine hydrochloride denaturation studies of mutant forms of staphylococcal nuclease

1986 • 85 citations

Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli

1987 • 67 citations

[1] Scanning calorimetry

1978 • 62 citations

Mutant sequences as probes of protein folding mechanisms

1987 • 29 citations