[17] Approaches to high-volume screening assays of viral polymerases and related proteins

A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system

1981 • 1,991 citations

Applications of Combinatorial Technologies to Drug Discovery. 1. Background and Peptide Combinatorial Libraries

1994 • 1,130 citations

Applications of Combinatorial Technologies to Drug Discovery. 2. Combinatorial Organic Synthesis, Library Screening Strategies, and Future Directions

1994 • 1,126 citations

Principles of fluorescence spectroscopy

1984 • 1,031 citations

Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

1988 • 847 citations

lac repressor-operator interaction

1970 • 791 citations

A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription

1981 • 703 citations

Thermodynamic Parameters To Predict Stability of RNA/DNA Hybrid Duplexes

1995 • 672 citations

Novel Fluorogenic Substrates for Assaying Retroviral Proteases by Resonance Energy Transfer

1990 • 589 citations

HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: Implications for HIV-1 latency

1991 • 422 citations

Sequence-specific RNA binding by the HIV-1 Rev protein

1989 • 384 citations

Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro

1989 • 377 citations

Reversal of Integration and DNA Splicing Mediated by Integrase of Human Immunodeficiency Virus

1992 • 376 citations

Time-Resolved Fluorescence of Lanthanide Probes and Applications in Biotechnology

1987 • 312 citations

Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis

1989 • 258 citations

Scintillation proximity assay

1989 • 221 citations

Fluorescence polarization — a new tool for cell and molecular biology

1995 • 185 citations

Inhibition of cap (m7GpppXm)-dependent endonuclease of influenza virus by 4-substituted 2,4-dioxobutanoic acid compounds

1994 • 182 citations

Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end

1991 • 176 citations

[12] Fluorescence anisotropy applied to biomolecular interactions

1995 • 162 citations

Recognition of the high affinity binding site in rev-response element RNA by the Human Immunodeficiency Virus type-1 rev protein

1992 • 119 citations

A novel assay for the DNA strand-transfer reaction of HIV-1 integrase

1994 • 117 citations

Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA

1977 • 114 citations

Scintillation proximity assay: A sensitive and continuous isotopic method for monitoring ligand/receptor and antigen/antibody interactions

1987 • 94 citations

Biochemical characterization of binding of multiple HIV-1 Rev monomeric proteins to the Rev responsive element

1993 • 76 citations

DNA substrate requirements for different activities of the human immunodeficiency virus type 1 integrase protein

1994 • 52 citations

A new protease activity assay using fluorescence polarization.

1994 • 46 citations

Solution oligomerization of the rev protein of HIV-1: Implications for function

1993 • 45 citations

A high-throughput, non-radioactive microtiter plate assay for activity of the human immunodeficiency virus integrase protein

1994 • 34 citations

Characterization of Endonucleolytic Activity of HIV-1 Integrase Using a Fluorogenic Substrate

1995 • 29 citations

Elucidation of Basic Mechanistic and Kinetic Properties of Influenza Endonuclease Using Chemically Synthesized RNAs

1996 • 21 citations

Time-resolved europium fluorescence in enzyme activity measurements: a sensitive protease assay.

1983 • 15 citations

Assay for Influenza Virus Endonuclease Using DNA Polymerase Extension of a Specific Cleavage Product

1995 • 3 citations

Fluorometric Measurement of Reverse Transcriptase Activity with 4′,6-Diamidino-2-phenylindole

1995 • 3 citations

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