Cultivation of Animal Cells in Chemically Defined Media, A Review
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Abstract
References (141)
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1950 • 1,066 citations
Some Aspects of the Biological Role of Adenosine 3',5'-monophosphate (Cyclic AMP)
1968 • 976 citations
An improved nutrient solution for diploid Chinese hamster and human cell lines
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Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells
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CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM
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Introduction to the polyamines
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The Growth in Vitro of Single Isolated Tissue Cells
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"Contact inhibition" of cell division in 3T3 cells.
1968 • 547 citations
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1966 • 484 citations
THE GROWTH RESPONSE OF MAMMALIAN CELLS IN TISSUE CULTURE TO l-GLUTAMINE AND l-GLUTAMIC ACID
1956 • 349 citations
Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2
1959 • 327 citations
THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN H<scp>E</scp>L<scp>A</scp>) IN TISSUE CULTURE
1955 • 320 citations
THE MINIMUM VITAMIN REQUIREMENTS OF THE L AND H<scp>E</scp>L<scp>A</scp> CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE
1955 • 318 citations
THE SPECIFIC AMINO ACID REQUIREMENTS OF A MAMMALIAN CELL (STRAIN L) IN TISSUE CULTURE
1955 • 279 citations
myo-INOSITOL AS AN ESSENTIAL GROWTH FACTOR FOR NORMAL AND MALIGNANT HUMAN CELLS IN TISSUE CULTURE
1957 • 268 citations
Modulation of Adenylate Cyclase Activity in Liver and Fat Cell Membranes by Insulin
1972 • 243 citations
The partial purification from calf serum of a fraction with multiplication‐stimulating activity for chicken fibroblasts in cell culture and with non‐suppressible insulin‐like activity
1972 • 209 citations
The Utilization of Carbohydrates by Human Cell Cultures
1958 • 206 citations
MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS: THE ACTION OF FETUIN IN PROMOTING CELL ATTACHMENT TO GLASS
1958 • 181 citations
Chemically defined media for cultivation of long-term cell strains from four mammalian species
1964 • 174 citations
PREPARATION FROM HUMAN SERUM OF AN ALPHA-ONE PROTEIN WHICH INDUCES THE IMMEDIATE GROWTH OF UNADAPTED CELLS IN VITRO
1967 • 173 citations
Serum Factor Requirements of Normal and Simian Virus 40-Transformed 3T3 Mouse Fibroblasts
1971 • 151 citations
EFFECT OF ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE ON CELL PROLIFERATION
1972 • 151 citations
An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions
1970 • 148 citations
Studies of nutrient media for tissue cells in vitro. I. A protein-free chemically defined medium for cultivation of strain L cells.
1956 • 146 citations
Stimulation of cell growth in vitro by serum with and without growth factor Relation to contact inhibition and viral transformation
1970 • 145 citations
Effect of Steroids on Growth of Mouse Fibroblastsin Vitro
1965 • 137 citations
The Ability of Purine and Thymine Derivatives and of Glycine to Support the Growth of Mammalian Cells in Culture
1959 • 136 citations
Putrescine and related amines as growth factors for a mammalian cell line
1963 • 134 citations
Requirements for Growth of Single Human Cells
1959 • 133 citations
Biochemistry of Cultured Mammalian Cells
1961 • 130 citations
Biochemistry of Differentiation
1968 • 130 citations
Dual Requirement of Walker Carcinosarcoma 256 in vitro for Asparagine and Glutamine
1956 • 121 citations
The requirement for L-asparagine of mouse leukemia cells L5178Y in culture.
1961 • 119 citations
A Protein Growth Factor for Mammalian Cells in Culture
1958 • 116 citations
Nutrition of Animal Cells in Tissue Culture. X. Synthetic Medium No. 858,
1955 • 97 citations
Cultivation of animal tissues in vitro in nutrients of precisely known constitution.
1946 • 96 citations
Hormonal Induction of Alkaline Phosphatase in Human Cells in Tissue Culture
1961 • 95 citations
Tyrosine Transaminase Induction by Dexamethasone in a New Rat Liver Cell Line
1970 • 94 citations
Molecular Growth Requirements of Single Mammalian Cells
1957 • 89 citations
The salt requirements of mammalian cells in tissue culture
1956 • 85 citations
Studies of nutrient media for tissue cells in vitro. II. An improved protein-free chemically defined medium for long-term cultivation of strain L-929 cells.
1956 • 83 citations
AN IMPROVED CHEMICALLY DEFINED BASAL MEDIUM (CMRL-1415) FOR NEWLY EXPLANTED MOUSE EMBRYO CELLS
1966 • 81 citations
STUDIES ON THE MECHANISM OF HORMONAL INDUCTION OF ALKALINE PHOSPHATASE IN HUMAN CELL CULTURES
1966 • 81 citations
Growth Factors for Mammalian Cells in Culture
1959 • 81 citations
Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian Cells
1963 • 75 citations
Protamine enhancement of RNA uptake by cultured chick cells
1961 • 73 citations
Growth-Promoting Properties of Pyruvate, Oxalacetate, and -Ketoglutarate For Isolated Walker Carcinosarcoma 256 Cells
1958 • 72 citations
MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS
1959 • 70 citations
Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media
1971 • 69 citations
Isolation of acrolein from incubated mixtures of spermine with calf serum and its effects on mammalian cells
1964 • 68 citations
Comparison of the Growth of Fibroblasts Under the Influence of 11β-Hydroxy and 11-Keto Corticosteroids
1966 • 66 citations
CELL CYCLE EVENTS IN THE HYDROCORTISONE REGULATION OF ALKALINE PHOSPHATASE IN HELA S3 CELLS
1969 • 66 citations
Action of Hydrocortisone on Cells in Tissue Culture.
1954 • 64 citations
CULTIVATION OF MAMMALIAN CELLS IN DEFINED MEDIA WITH PROTEIN AND NONPROTEIN SUPPLEMENTS
1966 • 61 citations
Nonidentity of Fetuin and Protein Growth (Flattening) Factor
1959 • 58 citations
THE SUSTAINED GROWTH OF HUMAN AND ANIMAL CELLS IN A PROTEIN-FREE ENVIRONMENT
1960 • 56 citations
Effects of spermine on mammalian cells
1961 • 54 citations
Growth of L-M Strain Mouse Cells in a Chemically Defined Medium.
1962 • 53 citations
Inhibitory Effects of Cortisone Acetate and Hydrocortisone on Growth of Fibroblasts.
1957 • 51 citations
NUTRITION OF ANIMAL CELLS IN TISSUE CULTURE: IX. SYNTHETIC MEDIUM No. 703
1954 • 49 citations
Bicarbonate as an essential for human cells in vitro
1958 • 46 citations
The mechanism of cell binding and cell-sheet formation in L strain fibroblasts
1960 • 46 citations
Purification of a serum protein required by a mammalian cell in tissue culture
1957 • 46 citations
Biochemical Aspects of Genetics
1965 • 43 citations
Prolonged survival of excised animal tissues in vitro in nutrients of known constitution
1949 • 43 citations
THE PURINE REQUIREMENT OF CELLS CULTURED FROM HUMANS AFFECTED WITH THE LESCH-NYHAN SYNDROME (HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE DEFICIENCY)
1969 • 43 citations
Clonal growth of diploid Chinese hamster cells in a synthetic medium supplemented with purified protein fractions
1962 • 43 citations
Effect of Citrovorum Factor and Peptones on Mouse Leukemia Cells L-5178 in Tissue Culture
1957 • 43 citations
The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts
1958 • 42 citations
A Chemically Defined Medium for Growth of Animal Cells in Suspension
1963 • 42 citations
Ascorbic Acid: A Culture Requirement for Colony Formation by Mouse Plasmacytoma Cells
1971 • 41 citations
Biotin, B12, and other vitamin requirements of a strain of mammalian cells grown in chemically defined medium
1963 • 40 citations
Studies on the nutrition and metabolism of animal cells in serum-free media: I. Serum-free monolayer cultures
1963 • 40 citations
The amino acid requirements of the Jensen sarcoma in vitro.
1959 • 40 citations
Growth and macromolecular synthesis in the 3T6 mouse fibroblast: I. general description and the role of ascorbic acid
1970 • 40 citations
Studies on the cultivation of mammalian cell lines in a serum-free, chemically defined medium
1973 • 40 citations
Actions of Thyroid Hormones on Cultured Human Cells
1966 • 40 citations
THE ACTION OF INSULIN ON THE METABOLISM OF CELL CULTURES
1960 • 39 citations
Improvements in a chemically defined medium for the growth of mouse cells (strain LS) in suspension
1970 • 37 citations
Factors affecting the production of glutamine in cultured mouse cells
1971 • 36 citations
The requirement of mammalian cell cultures for serum proteins. Growth-promoting activity of pepsin-digested serum albumin in different media
1967 • 35 citations
Suspension Culture of Mammalian Cells and Macromolecular Growth-Promoting Fractions of Calf Serum
1964 • 34 citations
Long-Term Cultivation of Human Cells (Chang) in Chemically Defined Medium and Effect of Added Peptone Fractions
1959 • 34 citations
Studies on the growth-promoting glycoprotein fraction of foetal calf serum
1962 • 33 citations
Some observations on the response of four established human cell strains to hydrocortisone in tissue culture.
1957 • 33 citations
CHOLESTEROL REQUIREMENT OF PRIMARY DIPLOID HUMAN FIBROBLASTS
1969 • 33 citations
An improved heat‐stable glutamine‐free chemically defined medium for growth of mammalian cells
1971 • 32 citations
Trace-Metal Requirements of NCTC Clone 929 Strain L Cells<xref ref-type="fn" rid="FN1">2</xref>
1967 • 32 citations
SERUM FRACTIONATION AND THE EFFECTS OF BOVINE SERUM FRACTIONS ON HUMAN CELLS GROWN IN A CHEMICALLY DEFINED MEDIUM
1961 • 32 citations
Effect of Chemically Defined Medium NCTC 109 Supplemented With Methocel and of Silicone Coating the Flasks on Strain 2071 Cells in Suspension Cultures<xref ref-type="fn" rid="FN1">2</xref>
1961 • 30 citations
Vitamin B12 dependent methionine biosynthesis in HEp-2 cells
1968 • 30 citations
The glucose, insulin and glutamine requirements of suspension cultures of hela cells in a defined culture medium
1971 • 30 citations
MAMMALIAN CELLS IN CHEMICALLY DEFINED MEDIA IN SUSPENSION CULTURES*
1966 • 29 citations
The Effect of Serum Fractions on the Proliferation of Strain L Mouse Cells <italic>in Vitro</italic>
1955 • 28 citations
The effect of oleic acid on the growth of monkey kidney cells (LLC-MK2)
1970 • 26 citations
Studies on the nature of serum stimulation of proliferation in cell culture
1970 • 25 citations
Summary of proceedings of a workshop on serum for tissue culture purposes
1971 • 25 citations
Binary Stars as Astronomical Accelerators
1972 • 25 citations
Carbon Dioxide Requirement and Nucleic Acid Metabolism of HeLa and Conjunctival Cells.
1961 • 24 citations
Vitamin requirements of uterine fibroblasts, strain U12-79; their replacement by related compounds
1958 • 23 citations
The stimulation of growth of mammalian cells in vitro by a peptide fraction from enzymatic digests of serum
1966 • 23 citations
Relative Growth-Promoting Activity in Tissue Culture of Go-Factors and the Parent Vitamins
1956 • 22 citations
Effect of Steroids on Human Cell Cultures; Sustaining Effect of Hydrocortisone.
1964 • 22 citations
The requirement for biotin in mouse fibroblast L-cells cultured on serumless medium
1969 • 21 citations
Stability of Vitamins in a Chemically Defined Medium for 929-L Fibroblasts<xref ref-type="fn" rid="FN2">2</xref>
1963 • 20 citations
THE AMINO ACID REQUIREMENTS OF RABBIT FIBROBLASTS, STRAIN RM3-56
1957 • 20 citations
Induction of Invertase Activity by Hydrocortisone in Chick Embryo Duodenum Cultures.
1966 • 20 citations
Effects of Albumin Fraction and Linoleic Acid On Growth of Macrophages in Tissue Culture2
1965 • 19 citations
Multiplication of Single Mammalian Cells in a Nonbicarbonate Medium
1960 • 19 citations
Growth and Metabolism of L Cells in a Chemically Defined Medium in a Controlled Environment Culture System
1971 • 17 citations
CATALASE REQUIREMENT FOR MAMMALIAN CELLS IN CULTURE
1958 • 16 citations
The Amino Acid Content of the Ultrafiltrate From Horse Serum<xref ref-type="fn" rid="fn1">2</xref>
1954 • 16 citations
An inhibitor present in calf serum which prevents growth of BHK21 cells in suspension culture
1972 • 16 citations
Cation requirements of HeLa cells in tissue culture
1961 • 15 citations
The effect of isomeric cis-octadecenoic acids on the growth of monkey kidney cells (LLC-MK2)
1970 • 15 citations
Iron Replacement of Lactalysate and Embryo Extract in Growth of Cell Cultures.
1961 • 15 citations
The quantitative glucose and mineral nutrient requirements of mouse LS (suspension) cells in chemically defined medium
1971 • 15 citations
Morphologic and functional effects of hydrocortisone in tissue culture.
1961 • 15 citations
Studies on the nutrition and metabolism of animal cells in serum-free media: II. Cultivation of cells in suspension
1963 • 14 citations
Growth and Metabolism of L Cells in a Chemically Defined Medium in a Controlled Environment Culture System
1971 • 14 citations
Minimal Vitamin Requirements of Rabbit Fibroblasts, Strain RM3-73
1957 • 12 citations
Adenosine 3′,5′-Monophosphate-Dependent Protein Kinase of Cultured Mammalian Cells
1971 • 11 citations
Extracts of plasma and serum toxic for mammalian cells cultured in vitro
1966 • 11 citations
THE AMINO ACID REQUIREMENTS OF A PERMANENT STRAIN OF ALTERED UTERINE FIBROBLASTS (U12-705)
1958 • 11 citations
Response of CO2-Deficient Human Cells in vitro to Normal Cell Extracts.
1958 • 10 citations
Probable Function of Macromolecules in Tissue Culture
1964 • 9 citations
Adaptation, Growth, and Chromosomal Analysis of HEp-2 Cells in Chemically Defined Medium<xref ref-type="fn" rid="FN1">2</xref>
1966 • 9 citations
The Free and Combined Amino Acids in the Protein-Free Ultrafiltrates from Whole Chick-Embryo Extracts<xref ref-type="fn" rid="fn1">2</xref>
1954 • 9 citations
Purine stimulation of Walker carcinosarcoma 256 in cell culture
1958 • 8 citations
Partial Replacement of Serum and Embryo Extract Requirements for Growth of Avian Cell Cultures.
1961 • 7 citations
Antioxidant activity of thyroxine and related substances
1968 • 7 citations
<italic>α</italic>-Keto Acids in Tissue Culture. I. The Concentration of Pyruvic and <italic>α</italic>-Ketoglutaric Acids in the Ultrafiltrates from Horse Serum and Chick-Embryo Extract
1955 • 7 citations
ACTIVITY OF VITAMIN B12 IN MAMMALIAN CELLS IN TISSUE CULTURE
1964 • 6 citations
Effect of glucocorticoid steroids on cells in tissue culture
1965 • 6 citations
The influence of the growth-promoting α-globulin on ion entrance into HeLa cells
1965 • 6 citations
Some Aspects of the Role of Vitamin B<sub>12</sub> in Nucleic Acid Synthesis in a Neoplastic C3H Mouse Cell Strain. I. Growth Studies
1970 • 5 citations
Pyrimidine Nucleoside Requirements of a Neoplastic C3H Mouse Cell Strain
1967 • 4 citations
Stimulatory Effects of Glycine, L-Serine, Folic Acid and Related Compounds on Growth of Cell Cultures.
1960 • 4 citations
The influence of the growth-promoting α-globulin on the CO2 and lactic acid production in cell culture
1966 • 2 citations
Some Aspects of the Role of Vitamin B<sub>12</sub> in Nucleic Acid Synthesis in a Neoplastic C3H Mouse Cell Strain. II. Incorporation of Precursors
1970 • 2 citations