Cysteine 148 in the Lactose Permease of Escherichia coli Is a Component of a Substrate Binding Site. 2. Site-Directed Fluorescence Studies

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli.

1965 • 374 citations

Lactose Carrier Protein of Escherichia coli Structure and Expression of Plasmids Carrying the Y Gene of the lac Operon

1980 • 262 citations

[32] Purification, reconstitution, and characterization of the lac permease of Escherichia coli

1986 • 174 citations

Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli

1993 • 113 citations

Construction of a functional lactose permease devoid of cysteine residues

1991 • 111 citations

Direct Measurement of the Binding of Labeled Sugars to the Lactose Permease M Protein

1974 • 96 citations

Properties and purification of an active biotinylated lactose permease from Escherichia coli.

1993 • 89 citations

Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli

1993 • 87 citations

Lac permease of Escherichia coli: arginine-302 as a component of the postulated proton relay

1987 • 86 citations

Site-directed mutagenesis of cys148 in the lac carrier protein of Escherichia coli

1984 • 85 citations

Frequency-domain fluorescence spectroscopy resolves the location of maleimide-directed spectroscopic probes within the tertiary structure of the calcium ATPase of sarcoplasmic reticulum

1991 • 63 citations

cys154 Is important for lac permease activity in Escherichia coli

1985 • 62 citations

Dynamics of Lactose Permease of Escherichia coli Determined by Site-Directed Fluorescence Labeling

1994 • 61 citations

Reaction of purified (Na,K)-ATPase with the fluorescent sulfhydryl probe 2-(4'-maleimidylanilino)naphthalene 6-sulfonic acid. Characterization and the effects of ligands.

1979 • 51 citations

Site-directed mutagenesis of cysteine-148 in the Lac permease of Escherichia coli: effect on transport, binding, and sulfhydryl inactivation

1985 • 47 citations

Kinetic analysis of lactose exchange in proteoliposomes reconstituted with purified lac permease

1991 • 41 citations

Properties of a mutant lactose carrier of Escherichia coli with a Cys¹⁴⁸ → Ser¹⁴⁸ substitution

1985 • 38 citations

Site-specific alteration of cysteine 176 and cysteine 234 in the lactose carrier of Escherichia coli.

1986 • 32 citations

Measurement of Fluorescence Lifetimes

1983 • 31 citations

Exchange, efflux and substrate binding by cysteine mutants of the lactose permease of Escherichia coli

1993 • 30 citations

Movement of Cys-697 in myosin ATPase associated with ATP hydrolysis.

1992 • 24 citations

Labeling of the beta gamma subunit complex of transducin with an environmentally sensitive cysteine reagent. Use of fluorescence spectroscopy to monitor transducin subunit interactions

1991 • 23 citations

A conformational change in the lactose permease of Escherichia coli is induced by ligand binding or membrane potential

1994 • 23 citations

Monitoring conformational change in the human erythrocyte glucose carrier: Use of a fluorescent probe attached to an exofacial carrier sulfhydryl

1993 • 20 citations

Reaction of (Na,K)-ATPase with fluorescent maleimide derivatives. Probes for studying ATP site(s) function.

1983 • 19 citations

Binding of p-nitrophenyl-.alpha.-D-galactopyranoside to lac permease of Escherichia coli

1990 • 13 citations

Reaction of the ArsA adenosinetriphosphatase with 2-(4'-maleimidoanilino)naphthalene-6-sulfonic acid

1993 • 11 citations

Substitution of glutamine-60 with glutamic acid causes the lac permease of Escherichia coli to become temperature sensitive

1986 • 8 citations