Prokaryotic secretion

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.

1975 • 1,175 citations

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle

1989 • 474 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.

1989 • 317 citations

Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export

1989 • 222 citations

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

1988 • 143 citations

In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides

1985 • 130 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Idealization of the hydrophobic segment of the alkaline phosphatase signal peptide

1986 • 98 citations

Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane

1989 • 90 citations

Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion

1990 • 77 citations

A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli

1989 • 69 citations

Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process.

1988 • 60 citations

Secretion and membrane assembly

1989 • 43 citations

The signal sequence of an Escherichia coli outer membrane protein can mediate translocation of a not normally secreted protein across the plasma membrane.

1987 • 36 citations

Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles

1986 • 33 citations

Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process

1989 • 22 citations