An in vivo library-versus-library selection of optimized protein–protein interactions

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

1985 • 3,886 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

X-Ray Structure of the GCN4 Leucine Zipper, a Two-Stranded, Parallel Coiled Coil

1991 • 1,433 citations

Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens

1997 • 886 citations

Erythropoietin Receptor Activation by a Ligand-Induced Conformation Change

1999 • 624 citations

Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDa FKBP12-rapamycin-associated protein and characterization of a critical serine residue.

1995 • 547 citations

A three-hybrid system to detect RNA-protein interactions in vivo.

1996 • 499 citations

Peptide ‘Velcro’: Design of a heterodimeric coiled coil

1993 • 437 citations

Sequence Requirements for Coiled-Coils: Analysis with λ Repressor-GCN4 Leucine Zipper Fusions

1990 • 379 citations

Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments

1998 • 366 citations

A protein linkage map of Escherichia coli bacteriophage T7

1996 • 338 citations

Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays

1999 • 228 citations

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis

1994 • 226 citations

The Role of Interhelical Ionic Interactions in Controlling Protein Folding and Stability

1994 • 189 citations

Electrostatic Interactions Control the Parallel and Antiparallel Orientation of .alpha.-Helical Chains in Two-Stranded .alpha.-Helical Coiled-Coils

1994 • 169 citations

Measurement of Interhelical Electrostatic Interactions in the GCN4 Leucine Zipper

1995 • 157 citations

Transient Kinetic Studies on the Interaction of Ras and the Ras-Binding Domain of c-Raf-1 Reveal Rapid Equilibration of the Complex

1998 • 129 citations

The net energetic contribution of interhelical electrostatic attractions to coiled-coil stability

1994 • 122 citations

Buried asparagines determine the dimerization specificities of leucine zipper mutants

1997 • 90 citations

The first constant domain (C_H1 and C_L) of an antibody used as heterodimerization domain for bispecific miniantibodies

1998 • 78 citations

A fully active variant of dihydrofolate reductase with a circularly permuted sequence

1992 • 75 citations

Thermodynamic Characterization of the Coupled Folding and Association of Heterodimeric Coiled Coils (Leucine Zippers)

1996 • 51 citations

Oligomerization properties of GCN4 leucine zipper e and g position mutants

1997 • 51 citations

Two pairs of oppositely charged amino acids from Jun and Fos confer heterodimerization to GCN4 leucine zipper.

1994 • 38 citations

Selectively infective phage (SIP) technology: A novel method for in vivo selection of interacting protein–ligand pairs

1997 • 35 citations

A phage‐based system to select multiple protein‐protein interactions simultaneously from combinatorial libraries

1998 • 11 citations