Four homologous domains in the primary structure of GrsB are related to domains in a superfamily of adenylate‐forming enzymes

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

1982 • 5,284 citations

Multiple sequence alignment with hierarchical clustering

1988 • 5,250 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

Construction of Phylogenetic Trees

1967 • 3,637 citations

Prediction of protein conformation

1974 • 3,327 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

1985 • 2,505 citations

The P-loop — a common motif in ATP- and GTP-binding proteins

1990 • 2,029 citations

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs

1990 • 1,345 citations

Molecules as documents of evolutionary history

1965 • 1,305 citations

[12] Unidirectional digestion with exonuclease III in DNA sequence analysis

1987 • 898 citations

Structure of E. coli Glutaminyl-tRNA Synthetase Complexed with tRNA ^Gln and ATP at 2.8 Å Resolution

1989 • 888 citations

Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering

1986 • 816 citations

Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases

1986 • 801 citations

A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 Å

1990 • 620 citations

Structure and regulation of rat long-chain acyl-CoA synthetase.

1990 • 358 citations

[23] Progressive alignment and phylogenetic tree construction of protein sequences

1990 • 350 citations

Nonribosomal biosynthesis of peptide antibiotics

1990 • 322 citations

A computer algorithm for testing potential prokaryotic terminators

1984 • 317 citations

Attempts to Map a Process Evolution of Peptide Biosynthesis

1971 • 262 citations

Gramicidin S biosynthesis operon containing the structural genes grsA and grsB has an open reading frame encoding a protein homologous to fatty acid thioesterases

1989 • 244 citations

The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes.

1990 • 240 citations

The multifunctional peptide synthetase performing the first step of penicillin biosynthesis in Penicillium chrysogenum is a 421,073 dalton protein similar to Bacillus brevis peptide antibiotic synthetases.

1990 • 184 citations

Characterization of the Cephalosporium acremonium pcbAB gene encoding alpha-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains

1991 • 182 citations

Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.

1990 • 179 citations

δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine Synthetase from Aspergillus nidulans

1989 • 168 citations

Novel Enzymic Hydrolytic Dehalogenation of a Chlorinated Aromatic

1991 • 168 citations

Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. Molecular characterization of the acvA gene encoding the first enzyme of the penicillin biosynthetic pathway

1991 • 164 citations

The predicted secondary structures of the nucleotide‐binding sites of six cation‐transporting ATPases lead to a probable tertiary fold

1989 • 163 citations

NMR studies of the magnesium-ATP binding site of adenylate kinase and of a 45-residue peptide fragment of the enzyme

1985 • 160 citations

Primary structures and catalytic properties of isoenzymes encoded by the two 4‐coumarate: CoA ligase genes in parsley

1988 • 159 citations

Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine

1991 • 147 citations

Cloning and expression in Escherichia coli of isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans

1988 • 141 citations

An active serine is involved in covalent substrate amino acid binding at each reaction center of gramicidin S synthetase.

1991 • 139 citations

PEPTIDYL TRANSFERS IN GRAMICIDIN S BIOSYNTHESIS FROM ENZYME-BOUND THIOESTER INTERMEDIATES

1969 • 136 citations

Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase.

1986 • 124 citations

The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum

1991 • 116 citations

Complete nucleotide sequence of the tycA gene coding the tyrocidine synthetase 1 fromBacillus brevis

1988 • 108 citations

Comparison and cross‐species expression of the acetyl‐CoA synthetase genes of the ascomycete fungi, Aspergillus nidulans and Neurospora crassa

1990 • 100 citations

Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus

1991 • 93 citations

Nucleotide sequence of the Escherichia coli entE gene

1989 • 89 citations

Molecular Cloning and Nucleotide Sequence of the Gramicidin S Synthetase 1 Gene1

1989 • 70 citations

A regulatory gene, angR, of the iron uptake system of Vibrio anguillarum: similarity with phage P22 cro and regulation by iron

1990 • 67 citations

The presence and possible role of phosphopantothenic acid in gramicidin S synthetase

1970 • 66 citations

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase.

1991 • 66 citations

Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1

1991 • 53 citations

Organization of the biosynthesis genes for the peptide antibiotic gramicidin S

1988 • 52 citations

Microcomputer programs for DNA sequence analysis

1982 • 52 citations

Two genes, atpC1 and atpC2, for the γ subunit of Arabidopsis thaliana chloroplast ATP synthase

1991 • 51 citations

Molecular cloning of an ornithine-activating fragment of the gramicidin S synthetase 2 gene from Bacillus brevis and its expression in Escherichia coli

1985 • 50 citations

Biochemical Studies on Gramicidin S Non-producing Mutants of Bacillus brevis ATCC 9999*

1974 • 46 citations

Enzyme-bound phosphopantetheine in tyrocidine biosynthesis

1970 • 41 citations

The Nucleotide Sequence for a Proline-Activating Domain of Gramicidin S Synthetase 2 Gene from Bacillus brevis1

1991 • 37 citations

The proline‐activating activity of the multienzyme gramicidin S synthetase 2 can be recovered on a 115‐kDa tryptic fragment

1990 • 29 citations

Characterization and Location of the L-Proline Activating Fragment from the Multifunctional Gramicidin S Synthetase 2

1991 • 27 citations

On the domain construction of the multienzyme gramicidin S synthetase 2

1990 • 23 citations

Proteinchemical and Kinetic Features of Gramicidin S Synthetase

1989 • 15 citations

Aminoacyl Transfer RNA Synthetases: General Scheme Of Structure Function Relationships In The Polypeptides And Recognition Of Transfer-RNAs

1987 • 3 citations