Hybridization histochemistry

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

A membrane-filter technique for the detection of complementary DNA

1966 • 3,996 citations

Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

1979 • 3,525 citations

Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing

1983 • 2,298 citations

A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane

1965 • 1,981 citations

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis

1981 • 1,881 citations

Detection of AIDS Virus in Macrophages in Brain Tissue from AIDS Patients with Encephalopathy

1986 • 1,694 citations

Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes

1984 • 1,638 citations

FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS

1969 • 1,214 citations

Techniques of Autoradiography

1967 • 1,151 citations

A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes

1984 • 1,066 citations

Immunological method for mapping genes on Drosophila polytene chromosomes.

1982 • 772 citations

Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.

1986 • 740 citations

Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes

1983 • 707 citations

Efficient transcription of RNA into DNA by avian sarcoma virus polymerase

1976 • 688 citations

Intracellular localization of messenger RNAs for cytoskeletal proteins

1986 • 639 citations

Expression in Brain of a Messenger RNA Encoding a Novel Neuropeptide Homologous to Calcitonin Gene-Related Peptide

1985 • 611 citations

RNA-DNA Hybrids at the Cytological Level

1969 • 592 citations

Autoradiographic localization of angiotensin II receptors in rat brain.

1984 • 569 citations

Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues

1985 • 568 citations

Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin

1985 • 526 citations

Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata

1986 • 492 citations

Detection of viral sequences of low reiteration frequency by in situ hybridization.

1978 • 458 citations

The engrailed locus of drosophila: In situ localization of transcripts reveals compartment-specific expression

1985 • 456 citations

An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex

1983 • 381 citations

Quantitative analysis ofin situhybridization methods for the detection of actin gene expression

1985 • 370 citations

Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use hybridization probes

1986 • 357 citations

SEQUENCE COMPLEMENTARITY OF T2-DNA AND T2-SPECIFIC RNA

1961 • 352 citations

Computerized densitometry and color coding of [¹⁴C] deoxyglucose autoradiographs

1980 • 345 citations

Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

1978 • 336 citations

The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis

1985 • 334 citations

A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochrome-labelled RNA

1980 • 325 citations

The Human Growth Hormone Gene Family: Nucleotide Sequences Show Recent Divergence and Predict a New Polypeptide Hormone

1982 • 312 citations

Transforming DNA integrates into the host chromosome

1981 • 311 citations

High resolution detection of DNA–RNA hybrids in situ by indirect immunofluorescence

1977 • 305 citations

Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.

1982 • 295 citations

Identification of proopiomelanocortin neurones in rat hypothalamus by in situ cDNA-mRNA hybridization

1983 • 279 citations

Analysis by immunocytochemistry and in situ hybridization of renin and its mRNA in kidney, testis, adrenal, and pituitary of the rat.

1986 • 250 citations

Detection of poly A⁺RNA in sea urchin eggs and embryos by quantitative in situ hybridization

1981 • 238 citations

Cloning and sequence analysis of cDNA for rat angiotensinogen.

1983 • 237 citations

Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers

1983 • 227 citations

Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligodeoxyribonucleotides

1986 • 220 citations

3′-End labeling of DNA with [α-32P] cordycepin-5′-triphosphate

1980 • 219 citations

Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: Implications for embryonic cell proliferation

1984 • 206 citations

Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor

1982 • 190 citations

Characterization and Localization of Proopiomelanocortin Messenger RNA in the Adult Rat Testis

1984 • 185 citations

Simultaneous in situ detection of viral RNA and antigens.

1984 • 179 citations

Localization of a unique gene by direct hybridization in situ.

1981 • 178 citations

In Situ Hybridization to Study the Origin and Fate of Identified Neurons

1983 • 169 citations

Autoradiographic Detection of Molecular Hybrids between rRNA and DNA in Tissue Sections

1970 • 165 citations

Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization.

1983 • 165 citations

In SituHybridization Histochemistry: A Technique for the Study of Gene Expression in Single Cells

1983 • 135 citations

In situ hybridization at the electron microscope level: hybrid detection by autoradiography and colloidal gold

1982 • 134 citations

Partial nucleotide sequence of human calcitonin precursor mRNA identifies flanking cryptic peptides

1982 • 130 citations

HYBRIDIZATION HISTOCHEMISTRY : USE OF RECOMBINANT DNA AS A “HOMING PROBE” FOR TISSUE LOCALIZATION OF SPECIFIC mRNA POPULATIONS.

1981 • 128 citations

Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.

1986 • 126 citations

Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization

1986 • 124 citations

Ross river virus 26 S RNA: Complete nucleotide sequence and deduced sequence of the encoded structural proteins

1983 • 123 citations

Detection of herpes simplex RNA in human sensory ganglia

1979 • 114 citations

Efficiency of in situ hybridization as a function of probe size and fixation technique

1985 • 111 citations

Hybridization histochemistry

1985 • 107 citations

Mouse glandular kallikrein genes. Identification, structure, and expression of the renal kallikrein gene.

1986 • 100 citations

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 • 97 citations

Accumulation of histone repeat transcripts in the sea urchin egg pronucleus

1981 • 85 citations

A non-radioactivein situhybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands

1986 • 85 citations

Neuropeptides: Mediators of Behavior in Aplysia

1984 • 84 citations

Evidence for and Localization of Vegetative Viral DNA Replication by Autoradiographic Detection of RNA·DNA Hybrids in Sections of Tumors Induced by Shope Papilloma Virus

1971 • 83 citations

Histone RNA in amphibian oocytes visualized by in situ hybridization to methacrylate-embedded tissue sections.

1984 • 81 citations

[34] In Situ hybridization for the study of gene expression in the brain

1986 • 80 citations

Correlation of grain counts with radioactivity (125I and tritium) in autoradiography

1966 • 74 citations

Detection of Two Viral Genomes in Single Cells by Double-Label Hybridization in Situ and Color Microradioautography

1985 • 72 citations

Biotin-Labeled Oligonucleotides: Enzymatic Synthesis and Use as Hybridization Probes

1984 • 68 citations

Sensitive detection of hybridocytochemical results by means of reflection-contrast microscopy.

1985 • 65 citations

Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis.

1985 • 65 citations

LOCALIZATION OF CELLS CONTAINING LHRH-LIKE mRNA IN RAT FOREBRAIN USINGIN SITUHYBRIDIZATION

1986 • 63 citations

Mouse glandular kallikrein genes. Nucleotide sequence of cloned cDNA coding for a member of the kallikrein arginyl esteropeptidase group of serine proteases.

1982 • 63 citations

Identification of individual interferon-producing cells by in situ hybridization.

1985 • 62 citations

The Secondary Structure and Poly(A) Content of Globin Messenger RNA as a Pure RNA and in Polyribosome-Derived Ribonucleoprotein Complexes

1975 • 59 citations

Tyrosine 3-hydroxylase in rat brain and adrenal medulla: hybridization histochemistry and immunohistochemistry combined with retrograde tracing.

1986 • 58 citations

In situ hybridization of putative somatostatin mRNA within hypothalamus of the rat using synthetic oligonucleotide probes

1985 • 56 citations

Detection of viral DNA and RNA by in situ hybridization.

1986 • 56 citations

Cytological Localization of Placental Lactogen Messenger Ribonviicleic Acid in Syncytiotrophoblast Layers of Human Placenta

1980 • 54 citations

Identification of Calcitonin and Calcitonin Gene-Related Peptide Messenger Ribonucleic Acid in Medullary Thyroid Carcinomas by Hybridization Histochemistry*

1986 • 54 citations

Molecular hybridization of 3H-labelled ribosomal RNA with DNA in ultrathin sections prepared for electron microscopy

1971 • 53 citations

Visualisation of messenger RNA directing peptide synthesis by in situ hybridisation using a novel single-stranded cDNA probe potential for the investigation of gene expression and endocrine cell activity

1984 • 51 citations

[35] In Situ cDNA;mRNA hybridization: Development of technique to measure mRNA levels in individual cells

1986 • 51 citations

Cytochemical hybridization with fluorochrome-labeled RNA. II. Applications.

1981 • 51 citations

The structure and biosynthesis of epidermal growth factor precursor

1985 • 47 citations

Detection of hepatitis b virus dna sequences in infected hepatocytes by in situ cytohybridisation

1981 • 47 citations

Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridization

1983 • 44 citations

Hemoglobin switching in sheep. Isolation of the fetal gamma-globin gene and demonstration that the fetal gamma- and adult beta A-globin genes lie within eight kilobase segments of homologous DNA.

1981 • 41 citations

[36] Location of gene expression in CNS using hybridization histochemistry

1986 • 39 citations

Complex formation between Poly-r (U) and various chromosomal loci in Rhynchosciara

1973 • 39 citations

Hybridization Histochemistry: Use of Recombinant DNA for Tissue Localizations of Specific mRNA Populations

1984 • 38 citations

Kallikrein gene expression in the rat anterior pituitary

1985 • 37 citations

Detection of the messenger RNA coding for preproenkephalin A in bovine adrenal by in situ hybridization

1984 • 36 citations

Detection of the messenger RNAs coding for the opioid peptide precursors in pituitary and adrenal by ‘in situ’ hybridization: Study in several mammal species

1985 • 36 citations

Characterization and Localization of Calcitonin Messenger Ribonucleic Acid in Rat Thyroid

1983 • 36 citations

Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization

1981 • 32 citations

The role of fetal adrenal hormones in the switch from fetal to adult globin synthesis in the sheep

1985 • 28 citations

Cytochemical hybridisation with fluorochrome-labelled RNA

1981 • 25 citations

An improved in situ hybridization method

1978 • 23 citations

Renin Gene Expression in Vessels of the Ovine Renal Cortex

1985 • 22 citations

A model for the organization of the poly(A) · protein complex in messenger ribonucleoprotein

1980 • 13 citations

In situ detection of vitamin D-induced calcium-binding protein (9-kDa CaBP) messenger RNA in rat duodenum.

1986 • 12 citations

Improved method for detection of cellular transcripts by in situ hybridization: Detection of poly (A) sequences in individual cells

1980 • 12 citations

USE OF SYNTHETIC OLIGONUCLEOTIDE AND RECOMBINANT DNA PROBES TO STUDY RENIN GENE EXPRESSION

1985 • 4 citations

Hybridization Histochemistry — Locating Gene Expression

1986 • 4 citations