Chapter 12 Microinjection of Mitotic Cells

The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio)

1995 • 6,935 citations

Drosophila — A Practical Approach

1987 • 1,888 citations

Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo

1995 • 940 citations

Analysis of P transposable element functions in drosophila

1984 • 733 citations

Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes.

1996 • 685 citations

Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.

1996 • 634 citations

Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.

1989 • 603 citations

Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.

1993 • 335 citations

Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.

1987 • 314 citations

Kinetochore microtubule dynamics and the metaphase-anaphase transition.

1995 • 292 citations

Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.

1992 • 271 citations

A Cellophane-Strip Technique for Culturing Tissue in Multipurpose Culture Chambers

1958 • 219 citations

CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase.

1994 • 200 citations

The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work.

1996 • 188 citations

Microinjection of Ca++-calmodulin causes a localized depolymerization of microtubules.

1983 • 181 citations

[74] Microinjection of early SV40 DNA fragments and T antigen

1980 • 176 citations

Chapter 2 Microinjection of Echinoderm Eggs: Apparatus and Procedures

1982 • 174 citations

CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo

1997 • 164 citations

Chromosome micromanipulation

1967 • 164 citations

Microtubule dynamics and chromosome motion visualized in living anaphase cells.

1988 • 147 citations

Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase.

1995 • 127 citations

Chapter 10 Incorporation of Macromolecules into Living Cells

1988 • 111 citations

A monoclonal antibody to a mitotic microtubule-associated protein blocks mitotic progression.

1990 • 107 citations

Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching.

1986 • 106 citations

Microinjection of Fluorescently Labeled Proteins into Living Cells with Emphasis on Cytoskeletal Proteins

1982 • 102 citations

Cell cycle-dependent, in vitro assembly of microtubules onto pericentriolar material of HeLa cells.

1979 • 93 citations

The distribution of calmodulin in living mitotic cells

1983 • 90 citations

Newt Lung Epithelial Cells: Cultivation, Use, and Advantages for Biomedical Research

1990 • 88 citations

NuMA, a nuclear protein involved in mitosis and nuclear reformation

1994 • 86 citations

Microinjection studies of duck globin messenger RNA translation in human and avian cells

1976 • 86 citations

Improved system for capillary microinjection into living cells

1982 • 85 citations

Chapter 12 Culturing Cells on the Microscope Stage

1988 • 61 citations

Actin filament translocations in sea urchin eggs

1996 • 55 citations

Dynamic aspects of microtubule biology.

1986 • 52 citations

Measurement of volume injected into individual cells by quantitative fluorescence microscopy

1989 • 49 citations

Microinjection of antibodies to a 62 kd mitotic apparatus protein arrests mitosis in dividing sea urchin embryos

1989 • 47 citations

Kinetochore microtubules shorten by loss of subunits at the kinetochores of prometaphase chromosomes

1991 • 44 citations

Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion.

1992 • 37 citations

Biotin-tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase.

1989 • 36 citations

Interzonal microtubules are dynamic during spindle elongation

1990 • 36 citations

Microtubule Dynamics in Mitotic Spindles of Living Cellsa

1986 • 28 citations

Nuclear concentration and mitotic dispersion of the essential cell cycle protein, p13suc1, examined in living cells.

1994 • 27 citations

Characterization of mitotic motors by their relative sensitivity to amp-pnp

1989 • 22 citations

Activation-dependent and activation-independent localisation of calmodulin to the mitotic apparatus during the first cell cycle of theLytechinus piçtusembryo.

1995 • 16 citations

[29] Microinjection into Xenopus oocytes

1995 • 8 citations

Expression of desmin cDNA in PtK2 cells results in assembly of desmin filaments from multiple sites throughout the cytoplasm

1992 • 6 citations

Micro injection of somatic cells

1980 • 3 citations

Mitotic arrest in PtK2 cells induced by microinjection of a rabbit antiserum and affinity-purified antibodies against a 66-kDa PtK2 cell polypeptide

1996 • 1 citations