An internally quenched fluorogenic substrate of prohormone convertase 1 and furin leads to a potent prohormone convertase inhibitor

Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen.

1992 • 688 citations

The new enzymology of precursor processing endoproteases.

1992 • 608 citations

Novel Fluorogenic Substrates for Assaying Retroviral Proteases by Resonance Energy Transfer

1990 • 589 citations

Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3).

1994 • 237 citations

A new fluorogenic substrate for chymotrypsin

1976 • 187 citations

Portion-mixing peptide libraries of quenched fluorogenic substrates for complete subsite mapping of endoprotease specificity.

1994 • 179 citations

Structure and function of eukaryotic proprotein processing enzymes of the subtilisin family of serine proteases.

1993 • 149 citations

Anthranilamide and nitrotyrosine as a donor-acceptor pair in internally quenched fluorescent substrates for endopeptidases: Multicolumn peptide synthesis of enzyme substrates for subtilisin carlsberg and pepsin

1991 • 145 citations

The convertases furin and PC1 can both cleave the human immunodeficiency virus (HIV)-1 envelope glycoprotein gp160 into gp120 (HIV-1 SU) and gp41 (HIV-I TM).

1994 • 135 citations

Intramolecularly quenched fluorogenic substrates for hydrolytic enzymes

1979 • 127 citations

Intramolecularly quenched fluorogenic tetrapeptide substrates for tissue and plasma kallikreins

1991 • 121 citations

A survey of furin substrate specificity using substrate phage display

1994 • 101 citations

Enzymic characterization of murine and human prohormone convertase-1 (mPC1 and hPC1) expressed in mammalian GH4C1 cells

1993 • 98 citations

Mammalian Neural and Endocrine Pro-Protein and Pro-Hormone Convertases Belonging to the Subtilisin Family of Serine Proteinases

1991 • 96 citations

Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease

1990 • 69 citations

Synthesis of a homologous series of ketomethylene arginyl pseudodipeptides and application to low molecular weight hirudin-like thrombin inhibitors

1992 • 62 citations

A new class of potent thrombin inhibitors that incorporates a scissile pseudopeptide bond

1991 • 47 citations

A fluorometric assay for HIV-protease activity using high-performance liquid chromatography

1990 • 39 citations

A quenched fluorescent substrate for thimet peptidase containing a new fluorescent amino acid, DL-2-amino-3-(7-methoxy-4-coumaryl)propionic acid

1991 • 38 citations

Design and synthesis of novel inhibitors of prohormone convertases

1994 • 33 citations

A continuous fluorimetric assay for clostridial collagenase and Pz-peptidase activity

1989 • 33 citations

Cleavage of proalbumin peptides by furin reveals unexpected restrictions at the P₂ and P′₁ sites

1994 • 25 citations

A Kex2-related endopeptidase activity present in rat liver specifically processes the insulin proreceptor

1994 • 25 citations

Kinetic analysis of the type-1 proinsulin endopeptidase by a monoclonal antibody-based immunoadsorbent assay

1992 • 17 citations

Biotinylated and Cysteine-Modified Peptides as Useful Reagents for Studying the Inhibition of Cathepsin G

1994 • 17 citations

Detection of femtomole quantities of proteases by high-performance liquid chromatography

1986 • 12 citations

A radiolabel-release microwell assay for proteolytic enzymes present in cell culture media

1990 • 10 citations

Biotinylation of an Enkephalin-Containing Heptapeptide via Various Spacer Arms. Synthesis, Comparative Binding Studies Toward Avidin, and Application as Substrates in Enzymic Reactions

1994 • 8 citations

Radiolabeled Biotinyl Peptides as Useful Reagents for the Study of Proteolytic Enzymes

1993 • 8 citations

Detection of endopeptidase activity and analysis of cleavage specificity using a radiometric solid-phase enzymatic assay

1991 • 8 citations

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