Shotgun Cloning as the Strategy of Choice to Generate Templates for High-throughput Dideoxynucleotide Sequencing

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

Fluorescence detection in automated DNA sequence analysis

1986 • 1,694 citations

Genomic mapping by fingerprinting random clones: A mathematical analysis

1988 • 955 citations

Gene Synthesis Machines: DNA Chemistry and Its Uses

1985 • 761 citations

Random subcloning of sonicated DNA: Application to shotgun DNA sequence analysis

1983 • 605 citations

Shotgun DNA sequencing using cloned DNase I-generated fragments

1981 • 425 citations

Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis

1987 • 328 citations

A sequence assembly and editing program for efficient management of large projects

1991 • 322 citations

Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver.

1989 • 300 citations

[7] Random cloning and sequencing by the M13/dideoxynucleotide chain termination method

1987 • 292 citations

Transposon-facilitated DNA sequencing.

1991 • 199 citations

Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

1988 • 117 citations

DNA Sequencing by Primer Walking with Strings of Contiguous Hexamers

1992 • 110 citations

[10] Enzymes for modifying and labeling DNA and RNA

1987 • 102 citations

Linear amplification sequencing, a powerful method for sequencing DNA

1991 • 97 citations

Closure strategies for random DNA sequencing

1991 • 54 citations

Nucleic acids sequencing : a practical approach

1989 • 46 citations

Optimization of asymmetric polymerase chain reaction for rapid fluorescent DNA sequencing.

1990 • 40 citations

Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.

1989 • 38 citations

Rapid preparation of bacteriophage DNA for sequence analysis in sets of 96 clones, using filtration

1986 • 33 citations

Strategies for rapid and accurate DNA sequencing

1991 • 31 citations

Rapid shotgeun cloning utillizing the two base recongition endonucleaseCviJI

1992 • 29 citations

Low pressure DNA shearing: a method for random DNA sequence analysis

1990 • 25 citations